INFLUENCE OF PERMANGANATE INDEX IN THE PARAMETERS AS TOTAL PHENOL CONTENT AND TOTAL ANTIOXIDANT ACTIVITY OF EXTRACTS OF CAMELLIA SINENSIS


Lucilene Dornelles Mello, Tiele Garcia Tunes

Abstract


Objective: This study estimated the Permangante Index (IKMnO4) and evaluated its correlation with parameters as total phenols (FT) and total antioxidant activity (TAA) of commercial teas of Camellia sinensis, fermented, unfermented and blended with other plants.

Methods: The aqueous extracts were prepared from commercial samples of green and black teas, green tea blended with lemon (Camellia sinensis with Citrus limonium), green tea blended with mint (Camellia sinensis with Mentha piperita), green tea blended with peach (Camellia sinensis with Prunus persica) and green tea blended with orange (Camellia sinensis with Citrus sinensis). The Permangante index was determined by titrating the tea extract with potassium permanganate solution according to AOAC method (AOAC, 1980) with modifications. The concentration of phenols in the extracts was determined by spectrophotometry according to the standard procedure of Folin-Ciocalteau. The antioxidant activity of the extracts was evaluated using the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. All the determinations were performed in triplicate, and the results presented as mean ± standard deviation (SD). The data were evaluated by univariate statistical analysis (ANOVA), F-test and mean test (Tukey´s test) for comparative results. Pearson’s correlation was used to measure the degree of linear correlation and the quantitative variables were from a normal population.

Results: The results for Permanganate Index show a variation ranged from 0.40 to 0.80 mEq/l. The ANOVA analysis to IKMnO4 shown there is significant statistically difference at the 5% probability level (p < 0.05) between the types of tea as well as the amount of herb used to prepare the infusion. Therefore, it was observed that there significant contrast between tea extracts, the mixture of different plants in blended extracts as well as the tea manufacture process affect the polyphenols tannins content influencing the permanganate index parameter. Studies correlation shown strong correlations (r > 0.7) among the parameters. For the Camellia sinensis extracts the high correlations between IKMnO4 and TAA; FT and TAA demonstrates the suitability of the permanganate index in the evaluation of antioxidant activity.

Conclusion: The results suggest that the Permanganate Index could be used for quality control of tea as an additional parameter.


Keywords


Permanganate Index, Camellia sinensis, Tea analysis, Bioactive constituents

| PDF | HTML |

References


Graham HN. Green tea composition, consumption and polyphenol chemistry. Prev Med 1992;12:334-50.

Chaturvedula VSP, Prakash I. The aroma, taste, color and bioactive constituents of tea. J Med Plants Res 2011;5:2110-24.

Sur P, Chaudhuri T, Vedasiromoni JR, Gomes A, Ganguly DK. Antiinflammatory and antioxidant property of saponins of tea (Camellia sinensis L. O. Kuntze) root extract. Phytother Res 2001;15:174-6.

Anita P, Sivasamy S, Kumar PDM, Balan IN, Ethiraj S. In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms. J Basic Clin Pharm 2015;6:35-9.

Zaverin N. Green tea and its polyphenolic catechins: medicinal uses in cancer and non-cancer applications. Life Sci 2006;78:2073-80.

Vijayd M, Anu Y. Anticancer activity of Camellia sinensis mediated copper nanoparticles against HT-29, MCF-7 and MOLT-4 human cancer cell lines. Asian J Pharm Clin Res 2017;10:71-7.

Saka S, Anshika NS, Sharma N. Potential anti-cancer superfoods: a mini-review. Int J Curr Pharm Res 2016;8:19-21.

Nakachi K, Matsuyamas MS, Suganuma M, Imai K. Preventive effects of drinking green tea on cancer and cardiovascular disease: epidemiological evidence for multiple targeting prevention. Life Sci 2000;13:49-54.

Pan T, Joseph J, Weidong LE. Beneficial therapeutic properties of green tea polyphenols in Parkins´s disease. Drugs Aging 2003;20:711-21.

Weinreb O, Mandel S, Amit T, Youdin MBH. Neurological mechanisms of green tea polyphenols in Alzheimer´s and Parkinson´s disease. J Nutr Biochem 2004;15:506-16.

Lambert JD, Elias RJ. The antioxidant and pro-oxidant activities of green tea polyphenols: a role in cancer prevention. Arch Biochem Biophys 2010;501:65-72.

Kale M, Suparna B, Pratima K. Role of antioxidant and nutrition in oxidation stress: a review. Int J Appl Pharm 2015;7:1-14.

AOAC. Official methods of analysis. Association of Analytical Chemists. Arlington, USA; 1980.

Rajkovic M, Sredovic ID. The determination of titratable acidity and total tannins in red wine. J Agric Sci 2009;54:223-46.

Ahmad TSS, Akhlaq N, Rahman K. Estimation of tannins in different food products. Int J Agric Biol 2001;3-4:529-30.

Pereira VP, Knor FJ, Vellosa JCR, Beltrame FL. Determination of phenolic compounds and antioxidant activity of green, black and white teas of Camellia sinensis (L.) Kuntze, Theaceae. Rev Bras Plant Med 2014;16:490-8.

Chan EWC, Soh EY, Tie PP, Law YP. Antioxidant and antibacterial properties of green, black and herbal teas of Camellia sinensis. Pharmacognosy Res 2011;3:266-72.

Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods Enzymol 1999;299:152-78.

Sunitha D. A review of antioxidant methods. Asian J Pharm Clin Res 2016;9:14-32.

Molyneux P. The use of the stable radical diphenylpicril-hydrazil (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26:211-9.

Al-Anbari AKH, Hasan MA. Antioxidant activity in some citrus leaves and seeds ethanolic extracts. Int Conf Adv Agric Biol Environ Sci 2015;93-7. http://dx.doi.org/10.15242/IICBE.C0715026.

Atanassova M, Christova-Bagdasssarian V. Determination of tannins content by titrimetric method for comparison of different plant species. J Univ Chem Technol Metall 2009;44:413-5.

Khasnabis J, Rai C, Roy A. Determination of tannin content by titrimetric method from different types of tea. J Chem Pharm Res 2015;7:238-41.

Kulisic T, Dragovic-Uzeloc V, Milos M. Antioxidant activity of aqueous tea infusions prepared from oregano, thyme and will thyme. Food Technol Biotechnol 2006;44:485-92.

Dasanayake P, Soysa P. Distribution of phenolic content and screening for antioxidant capacity of different parts of tea plant (Camellia sinensis L.). J Med Plants Res 2016;10:570-4.

Astill C, Birch MR, Dacome C, Humphrey PG, Martin PT. Factors affecting the caffeine and polyphenols contents of black and green tea infusions. J Agric Food Chem 2001;49:5340-7.

Rusak G, Komes D, Likic S, Horzic D, Kovac M. Phenolic content and antioxidant capacity of green and white tea extract depending on extraction conditions and the solvent used. Food Chem 2008;110:852-8.

Senanayake SPJN. Green tea extract: chemistry, antioxidant properties and food applications–a review. J Funct Foods 2013;5:1529-41.

Stodt U, Engelhardt UH. Progress in the analysis of selected tea constituents over the past 20 y. Food Res Int 2013;53:636-48.

Kaur L, Jayasekera S, Moughan PJ. Antioxidant quality of tea (Camellia sinensis) as affected by environmental factors. Chapter 13. In: Preedy V. editor. Processing and Impact on antioxidants in beverages. Oxford: Academic Press; 2014. p. 121-9.




About this article

Title

INFLUENCE OF PERMANGANATE INDEX IN THE PARAMETERS AS TOTAL PHENOL CONTENT AND TOTAL ANTIOXIDANT ACTIVITY OF EXTRACTS OF CAMELLIA SINENSIS

Keywords

Permanganate Index, Camellia sinensis, Tea analysis, Bioactive constituents

DOI

10.22159/ijpps.2017v9i8.19270

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 8, 2017 Page: 110-113

Online ISSN

0975-1491

Statistics

54 Views | 35 Downloads

Authors & Affiliations

Lucilene Dornelles Mello
Area of Chemistry, Federal University of Pampa (UNIPAMPA), Campus Bagé, Bagé, Rio Grande do Sul, Brasil
Brazil

Tiele Garcia Tunes
Area of Chemistry, Federal University of Pampa (UNIPAMPA), Campus Bagé, Bagé, Rio Grande do Sul, Brasil


Article Tools



Refbacks

  • There are currently no refbacks.