SCREENING OF THYMUS VULGARIS ESSENTIAL OIL AGAINST FUNGI CAUSING DERMATOPHYTOSIS IN HUMAN BEINGS
Keywords:Thymus vulgaris, Essential oil, Dermatophytes, Trichophyton, Microsporum
Objective: The study was designed to determined antidermatophytic activity of Thymus vulgaris essential oil against fungi causing superficial skin infections in human and animal.
Methods: Minimum inhibitory concentration (MIC) of essential oil was screened against selected pathogenic fungi namely Trichophyton rubrum (MTCC 296), T. mentagrophytes (MTCC 7687), Microsporum gypseum (MTCC 4524), M. fulvum(MTCC2837), T. soudanense and T. interdigitale through semi solid agar antifungal susceptibility testing method(SAAS). Minimum fungicidal concentration (MFC) was also determined by modified semi solid agar antifungal susceptibility method. T. soudanense and T. interdigitalis were isolated through TO. KA. VA hair bating technique from animal habitats soil of Jaipur district.
Results: T. vulgaris essential oil exhibited excellent antideramtophytic activity against all selected dermatophytes. Minimum inhibitory concentration was ranged from 0.020Â±0.000Î¼l/ml to 0.1Â±0.033 Î¼l/ml. MFC were found little higher than MIC (0.02Â±0.000Î¼l/ml to.2Â±0.000Î¼l/ml). M. gypseum was found to be most susceptible fungus as compared to other test fungi.
Conclusion: T. vulgaris was found to be most effective fungicidal agent against human pathogenic fungi. Present findings provide for a rationale basis of a possible utilization of this oil in fields requiring safe and cheap compounds with antiseptic and preservative properties, such as cosmetic, pharmaceutical and food industries.
Weitzman I, Summerbell R. The dermatophytes. Clin Microbiol 1995;8:240-59.
Maraki S, Tselentis Y. Dermatophytoses in crete, greece between 1992 and 1996. Mycoses 1998;41:175-8.
Ameen M. Epidemiology of superficial fungal infections. Clin Dermatol 2010;28:197-201.
Newze EI. Dermatophytoses in Western Africa: a reivew. Pak J Biol Sci 2010;13:649-56.
Jain N, Sharma M, Sharma M, Saxena VN. Spectrum of dermatophytoses in Jaipur, India. Afr J Microbiol Res 2014;8:237-43.
Bhatia VK, Sharma CP. Epidemiological studies on dermatophytosis in human patients in Himachal Pradesh, India. Springler Plus 2014;3:134.
Petmy LJ, Lando AJ, Kaptue L, Tchinda V, Folefack M. Superficial mycoses and HIV infection in Yaounda. J Eur Acad Dermatol Venerol 2004;8:301-4.
Watanabe S. Present state and future direction of topical antifungals. Japaneses J Med Mycol 1990;40:151-5.
Mandeel QA, Al-Laith AA. Ethnomycological aspects of the desert truffle among native Bahraini and non-Bahraini peoples of the Kingdom of Bahrain. J Ethnopharmacol 2007;110:118-29.
Jain N, Sharma M. Broad spectrum antimycotic drug for the treatment of ring worm infection in human beings. Curr Sci 2003;85:30-4.
Shahi SK, Shahi MP, Choudhary S, Kumar M. Broad spectrum herbal antifungal compound for the cure of human dermatophytoses. Plant Arch 2007;7:771â€“3.
Kalita L, Dash B, Borah U, Deka J, Dash S. Preliminary phytochemical analysis and antimicrobial activity ethanolic extracts of dried fruits of Solanum torvum (family-Solanaceae). Int J Curr Pharm Res 2017;9:123-6.
Jalila El Amri, Khalid El Badaoui, Zoubida Halouia. The chemical composition and the antimicrobial properties of the essential oil extracted from the leaves of Teucrium capitatum L. Asian J Pharm Clin Res 2017;10:112-5.
Jain N, Sharma M. Screening of Lawsonia inermis essential oil against fungi causing dermatophytic infection in human. Asian J Pharm Clin Res 2016;9:67-9.
Van Vuuren SF, Suliman S, Viljoen AM. The antimicrobial activity of four commercial essential oils in combination with conventional antimicrobials. Lett Appl Microbiol 2009;48:440-6.
Ali Ahmad Al Maqtari M, Alghalibi SM, Alhamzy EH. Chemical composition and antimicrobial activity of essential oil of Thymus vulgaris from Yemen. Turk J Biochem 2011;36:342-9.
Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol 1999;86:985-90.
Duke OS, Dayan EF, Romagani JG, Rimando MA. Natural products as sources of herbicides current status and future trends. Weed Res 2000;40:99-111.
Bremnes L. Herbs. Eyewitness-Handbooks; DK Publishing: New York, USA; 2002.
Rota MC, Herrera A, MartÃnez RM, Sotomayor JA, JordÃ¡n MJ. Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. Food Control 2008;19:681-7.
Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, et al. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chem 2005;91:621-32.
Lira Mota KS, Oliveira Pereia F, Oliveira WA. Antifungal activity of Thymus vulgaris L. essential oil and its constituent phytochemicals against Rhizopus oryzae: interaction with ergosterol. Molecules 2012;17:14418-33.
Rus C, Sumalan RM, Alexa E, Copolovici DM, Pop G, Botau D. Study on chemical composition and antifungal a ctivityof essential oils obtained from representative species belonging to the Lamiaceae family. Plant Soil Environ 2015;61:297â€“302.
Fani MM, Jamshid Kohanteb. In vitro antimicrobial activity of Thymus vulgaris essential oil against major oral pathogens. J Evidence-Based Complementary Altern Med 2017;1-7. Doi:10.1177/2156587217700772
Vanbreuseghem R. Technique biologique pour l'isolment des dermatophytes du sol (Biological technique for the isolation of dermatophytes from the soil). Ann Soc Belge Med Trop 1952;32:173Â¬-8.
Conant Norman F, Smith David T, Baker Roger D, Callaway Jasper L, Martin Donald S. Mycology of dermatomycoses. In: Manual of Clinical Mycology, Philadelphia: W. A. Saunders company; 1959. p. 329-52.
Forbes Betty A, Sahm Daniel F, Weissfeld Alice S. Laboratory methods in basic mycology. Bailey and Scottâ€™s Diagnostic Microbiology (Eleventh edition). Mosby, Inc; 2002. p. 711-97.
Provine H, Hadley S. Preliminary evaluation of a semisolid agar antifungal susceptibility test for yeast and molds. J Clin Microbiol 2002;38:537-41.
SokoviÄ‡ MD, Jelena VukojeviÄ‡, Petar D Marin, Dejan D BrkiÄ‡, Vlatka Vajs, Leo J L D van Griensven. Chemical composition of essential oils of Thymus and Mentha species and their antifungal activities. Molecules 2009;14:238-49.
Boruga O, Jianu C, Misca C, GoleÅ£ I, Gruia AT, Horhat FG. Thymus vulgaris essential oil: chemical composition and antimicrobial activity. J Med Life 2014;7:56-60.
Shigeharu Inouye, Katsuhisa Uchida, Shigeru Abe. Vapor activity of 72 essential oils against a Trichophyton mentagrophytes. J Infect Chemother 2006;12:210â€“6.
Pina-Vaz C, Goncalves Rodrigues A, Pinto E, S Costa-de-Oliveira, C Tavares, L Salgueiro, et al. Antifungal activity of Thymus oil and their major compounds. J Eur Acad Dermatol Venereol 2004;18:73-8.
Adams S, Kunz B, WeidenbÃ¶rner M. Mycelial deformations of Cladosporium herbarum due to the application of Eugenol and Carvacrol. J Essent Oil Res 1996;8:535-40.
Adams K, Sivropoulou A, Kokkini S, Lanaras T, Arsenakis M. Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia and Salvia fruticosa essential oils against human pathogenic fungi. J Agric Food Chem 1998;46:1738-45.
Shapiro S, Guggenheim B. The action of thymol on oral bacteria. Oral Microbiol Immunol 1995;10:241-6.
Dorman HJD, Deans SG. Antimicrobial agents from plants: antimicrobial activity of plant volatile oils. J Appl Microbiol 2000;88:308-16.
Xu J, Zhou F, Ji BP. The antibacterial mechanism of carvacrol and thymol against Escherichia coli. Lett Appl Microbiol 2008;47:174-9.
Ahmad A, Vuuren SV, Viljoen A. Unraveling the complex antimicrobial interactions of essential oilsâ€”the case of Thymus vulgaris (thyme). Molecules 2014;19:2896-910.