SCREENING OF THYMUS VULGARIS ESSENTIAL OIL AGAINST FUNGI CAUSING DERMATOPHYTOSIS IN HUMAN BEINGS
Objective: The study was designed to determined antidermatophytic activity of Thymus vulgaris essential oil against fungi causing superficial skin infections in human and animal.
Methods: Minimum inhibitory concentration (MIC) of essential oil was screened against selected pathogenic fungi namely Trichophyton rubrum (MTCC 296), T. mentagrophytes (MTCC 7687), Microsporum gypseum (MTCC 4524), M. fulvum(MTCC2837), T. soudanense and T. interdigitale through semi solid agar antifungal susceptibility testing method(SAAS). Minimum fungicidal concentration (MFC) was also determined by modified semi solid agar antifungal susceptibility method. T. soudanense and T. interdigitalis were isolated through TO. KA. VA hair bating technique from animal habitats soil of Jaipur district.
Results: T. vulgaris essential oil exhibited excellent antideramtophytic activity against all selected dermatophytes. Minimum inhibitory concentration was ranged from 0.020Â±0.000Î¼l/ml to 0.1Â±0.033 Î¼l/ml. MFC were found little higher than MIC (0.02Â±0.000Î¼l/ml to.2Â±0.000Î¼l/ml). M. gypseum was found to be most susceptible fungus as compared to other test fungi.
Conclusion: T. vulgaris was found to be most effective fungicidal agent against human pathogenic fungi. Present findings provide for a rationale basis of a possible utilization of this oil in fields requiring safe and cheap compounds with antiseptic and preservative properties, such as cosmetic, pharmaceutical and food industries.
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