GANODERMA LUCIDUM-ORIENTAL MUSHROOM MEDIATED SYNTHESIS OF GOLD NANOPARTICLES CONJUGATED WITH DOXORUBICIN AND EVALUATION OF ITS ANTICANCER POTENTIAL ON HUMAN BREAST CANCER MCF-7/DOX CELLS


D. S. Ranjith Santhosh Kumar, P. Senthilkumar, L. Surendran, B. Sudhagar

Abstract


Objective: The present investigations are to mycosynthesis and characterization of gold nanoparticles conjugated with doxorubicin and evaluated anticancer activity.

Methods: The characterization of the gold nanoparticles using ultraviolet–visible spectroscopy. FTIR investigations were carried out to find and read the functional group responsible designed at the bioconversion of gold ions and crystalline arrangement of gold nanoparticles was detected in the XRD study. The gold nanoparticles conjugated with doxorubicin were treated against MCF-7-dox resisted breast cancer cells and observed the in vitro cytotoxicity by MTT assay, SCGE (Comet), Apoptosis and Mito-potential assay. Further more we determinate the mRNA expression of ABCB1 gene and cDNA was synthesized from the mRNA for amplification of the ABCB1 gene corresponding to the specific primer.

Results: Surface Plasmon resonance showed the development of gold nanoparticles in UV–Visible spectra at 537 nm. The synthesized gold nanoparticles were polydisperse spherical and it was determined by EDAX and stabilized in the solution to the spherical shapes further confirmed by High-resolution transmission electron microscope analysis designate in the reading of 2–100 nm. The anticancer assays were given significant results and the mRNA expression of ABCB1 gene and cDNA was amplified as directly proportional to the expression of ABCB1 gene.

Conclusion: We propose that gold nanoparticles synthesized and conjugated with doxorubicin from G. lucidum might be a significant resource of drug delivery for anti-cancer preparation that may advantage breast cancer treatment.


Keywords


Oriental mushroom, Ganoderma lucidum, Doxorubicin, Au-NPs, 2–100 nm, Breast cancer MCF-7/Dox cells

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About this article

Title

GANODERMA LUCIDUM-ORIENTAL MUSHROOM MEDIATED SYNTHESIS OF GOLD NANOPARTICLES CONJUGATED WITH DOXORUBICIN AND EVALUATION OF ITS ANTICANCER POTENTIAL ON HUMAN BREAST CANCER MCF-7/DOX CELLS

Keywords

Oriental mushroom, Ganoderma lucidum, Doxorubicin, Au-NPs, 2–100 nm, Breast cancer MCF-7/Dox cells

DOI

10.22159/ijpps.2017v9i9.20093

Date

22-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 9, 2017 Page: 267-274

Online ISSN

0975-1491

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Authors & Affiliations

D. S. Ranjith Santhosh Kumar
PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore-29, Tamilnadu, India
India

P. Senthilkumar
PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore-29, Tamilnadu, India
India

L. Surendran
PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore-29, Tamilnadu, India
India

B. Sudhagar
PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore-29, Tamilnadu, India
India


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