ANTIMICROBIAL ACTIVITY AND TLC PROFILING OF CLERODENDRUM PHLOMIDIS LINN. F LEAF EXTRACT AGAINST MULTI-DRUG RESISTANT CLINICAL PATHOGENS

  • A. Hannah Hepsibah Research Scholar, Department of Plant Biology and Biotechnology, Loyola College, Chennai—600034, Tamil Nadu, India
  • M. Mala Research Scholar, Department of Plant Biology and Biotechnology, Loyola College, Chennai—600034, Tamil Nadu, India
  • G. Jeya Jothi Assistant Professor, Department of Plant Biology and Biotechnology, Loyola College, Chennai—600 034, Tamil Nadu, India

Abstract

Objective: The aim of the present study is to evaluate the antibacterial and antifungal activities of different solvent extracts of leaves of Clerodendrum phlomidis against multi-drug resistant clinical pathogens and to perform Thin Layer Chromatography (TLC) profiling of the extracts.

Methods: The leaf powder was sequentially extracted with petroleum ether, ethyl acetate, acetone, and methanol. The extracts were tested for their antimicrobial activity against 20 bacterial strains and 15 fungal strains which were multiple drugs resistant by disc diffusion method. TLC profiling of all four extracts was performed.

Results: The petroleum ether and ethyl acetate extracts showed antimicrobial activity against four bacteria and seven fungal strains. The highest zone of inhibition (18±0.50 mm) for bacteria was recorded against Staphylococcus epidermis by ethyl acetate extract and for fungi, the petroleum ether extract showed 13±0.00 mm against Trichophyton rubrum. TLC profiles showed that ethyl acetate extract possessed more compounds.

Conclusion: From the results of the present study it is concluded that the petroleum ether and ethyl acetate extracts of C. phlomidis leaves are good sources of antimicrobial compounds which can inhibit multiple drug resistant pathogens.

Keywords: Antimicrobial activity, Disc diffusion, Multiple drug resistant pathogens, TLC profiling, Clerodendrum phlomidis

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Hepsibah, A. H., M. Mala, and G. J. Jothi. “ANTIMICROBIAL ACTIVITY AND TLC PROFILING OF CLERODENDRUM PHLOMIDIS LINN. F LEAF EXTRACT AGAINST MULTI-DRUG RESISTANT CLINICAL PATHOGENS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 9, no. 9, Sept. 2017, pp. 275-8, doi:10.22159/ijpps.2017v9i9.20186.
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