ANTIMICROBIAL ACTIVITY AND TLC PROFILING OF CLERODENDRUM PHLOMIDIS LINN. F LEAF EXTRACT AGAINST MULTI-DRUG RESISTANT CLINICAL PATHOGENS
Objective: The aim of the present study is to evaluate the antibacterial and antifungal activities of different solvent extracts of leaves of Clerodendrum phlomidis against multi-drug resistant clinical pathogens and to perform Thin Layer Chromatography (TLC) profiling of the extracts.
Methods: The leaf powder was sequentially extracted with petroleum ether, ethyl acetate, acetone, and methanol. The extracts were tested for their antimicrobial activity against 20 bacterial strains and 15 fungal strains which were multiple drugs resistant by disc diffusion method. TLC profiling of all four extracts was performed.
Results: The petroleum ether and ethyl acetate extracts showed antimicrobial activity against four bacteria and seven fungal strains. The highest zone of inhibition (18Â±0.50 mm) for bacteria was recorded against Staphylococcus epidermis by ethyl acetate extract and for fungi, the petroleum ether extract showed 13Â±0.00 mm against Trichophyton rubrum. TLC profiles showed that ethyl acetate extract possessed more compounds.
Conclusion: From the results of the present study it is concluded that the petroleum ether and ethyl acetate extracts of C. phlomidis leaves are good sources of antimicrobial compounds which can inhibit multiple drug resistant pathogens.
2. Sumathi, Parvathi. Antimicrobial activity of some traditional medicinal plants. J Med Plant Res 2010;4:316-21.
3. Zaidan MRS, Rain NA, Badrul AR. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Trop Biomed 2005;22:16570.
4. Sathish M, Priyadarsini R, Sunitha PG, Saraswathy T. Antimicrobial activity of the extracts and isolated compounds Of Clerodendrum phlomidis. Int J Pharm Pharm Sci 2013; 5:362-6.
5. Neeta Shrivastava, Tejas Patel. Clerodendrum and healthcare: an overview. Med Aromat Plant Sci Biotechnol 2007;1:142-50.
6. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.
7. Hannah Hepsibah A, Jeya Jothi G. In vitro antibacterial activity and phytochemical analysis of Ormocarpum cochinchinense extracts on nosocomial infection causing bacteria. Asian J Pharm Clin Res 2014;8:113â€“6.
8. Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas. Springer Science and Business Media; 1996.
9. WHO. Antimicrobial Resistance, Global Report on Surveillance. Geneva: WHO; 2014.
10. Mohan Maruga Raja MK, Mishra SH. A comprehensive review of Clerodendrum phlomidis. J Chin Integr Med 2010;8:510-24.
11. Ncube NS, Afolayan AJ, Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. Afr J Biotechnol 2008;7:1797-806.
12. Balaji K, Kilimozhi D. GC-MS analysis of various extracts of Clerodendrum phlomidis leaf. Int J Pharm Pharm Sci 2014;6:226-32.
13. Sathish M, Tharani CB, Niraimathi V, Satheesh Kumar D. In vitro antioxidant activity of phenolic and flavonoid compounds extracted from the roots of C. phlomidis. Int J Pharm Pharm Sci 2012;4:288-91.