• Prashith Kekuda T. R. Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
  • Raghavendra H. L. Department of Biochemistry, School of Medicine, Wollega University, P.O. Box: 395, Nekemte, Ethiopia
  • Shilpa M. Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
  • Pushpavathi D. Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
  • Tejaswini Petkar Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
  • Ayesha Siddiqha Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India



Gardenia gummifera L f, Agar well diffusion, Poisoned food technique, DPPH, ABTS, Insecticidal, Aedes aegypti


Objective: The present study was carried out to investigate antimicrobial, antiradical and insecticidal potential of leaf and fruit of Gardenia gummifera L. f. (Rubiaceae).

Methods: The leaf and fruits were shade dried, powdered and extracted by maceration process using methanol. Antibacterial activity was evaluated against Gram positive and Gram negative bacteria by Agar well diffusion assay. Antifungal activity was determined against six seed-borne fungi by Poisoned food technique. Antiradical activity of leaf and fruit extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) radical scavenging assays. Insecticidal activity of leaf and fruit extracts, in terms of larvicidal and pupicidal activity, was assessed against larvae and pupae of Aedes aegypti.

Results: Both the extracts inhibited all test bacteria. Marked antibacterial activity was displayed by fruit extract when compared to leaf extract. S. epidermidis and E. coli were inhibited to highest and least extent by both extracts respectively. Fruit extract was found to exhibit higher antifungal effect when compared to leaf extract. Leaf extract and fruit extract exhibited highest inhibitory activity against A. niger and A. flavus respectively. Leaf and fruit extracts scavenged DPPH radical's dose dependently with an IC50 value of 49.01µg/ml and 2.53µg/ml respectively. The scavenging of ABTS by leaf and fruit extracts was dose dependent and the IC50 value for leaf and fruit extract was 2.58µg/ml and 2.31µg/ml respectively. Fruit extract was shown to exhibit marked antiradical activity when compared to leaf extract. Leaf and fruit extracts exhibited dose dependent insecticidal activity in terms of larvicidal and pupicidal activity and the susceptibility of larvae and pupae to extracts was in the order II instar larvae>IV instar larvae>pupae. Fruit extract displayed marked insecticidal potential when compared to leaf extract.

Conclusion: Overall, fruit extract of G. gummifera exhibited marked antimicrobial, antiradical and insecticidal activity when compared to leaf extract. The plant can be used for developing agents/formulations effective against infectious microorganisms, oxidative stress and insect vectors that transmit dreadful diseases. The observed bioactivities could be ascribed to the presence of active principles which are to be isolated and characterized.


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Parmar VS, Sharma SK, Poonam. Novel constituents of Gardenia species-a review. J Sci Indian Res 2000;59:893-903.

Ramabharathi V, Rao AVNA, Rajitha G. Phytochemical investigation and evaluation of antibacterial and antioxidant activities of leaf-bud exudate of Tarenna asiatica (L.) Kuntze ex K. Schum. Indian J Nat Prod Resour 2014;5:48-51.

Khare CP. Indian herbal remedies: rational western therapy, Ayurvedic and other traditional usage, Botany. Springer-Verlag, Berlin; 2004.

Bhat GK. Flora of South Kanara. Akriti Prints, Mangalore, India; 2014.

Patwari SG, Harikiran L, Rao AAVN, Reddy NY. Evaluation of anti-cancer activity of dikamaliartane-a, a cycloartane isolated from dikamali, a gum resin. Int J Pharm Pharm Sci 2012;4:501-4.

Manjunatha BK, Krishna V, Pullaiah T. Flora of davanagere district, Karnataka, India. Regency Publications, New Delhi, India; 2004.

Prasad MNV, Padmalatha K, Jayaram K, Raju NL, da Silva JAT. Medicinal plants from Deccan ecoregion, India: Traditional knowledge, ethnopharmacology, cultivation, utilization, conservation and biotechnology-opportunities and impediments. Med Aromatic Plant Sci Biotechnol 2007;1:155-208.

Tambekar DH, Khante BS. Antibacterial evaluation of medicinal plants used by Korkus in Melghat forest against gastrointestinal infections. Int J Pharm Sci Res 2011;2:577-83.

Shanmugam S, Annadurai M, Rajendran K. Ethnomedicinal plants used to cure diarrhea and dysentery in Pachalur hills of Dindigul district in Tamil Nadu, Southern India. J Appl Pharm Sci 2011;1:94-7.

Sammaiah D, Chandraiah G, Devi AU, Rajesham KC, Ugandhar T. Progress and prospects of medicinal plants of ethnopediatric importance in mahadevapur reserve forest of Karimnagar East division of (A. P.) India. Biosci Discovery 2013;4:173-5.

Savithramma N, Yugandhar P, Babu HR, Prasad SK. Validation of indigenous knowledge of Yanadi tribe and local villagers of Veyilingalakona-A sacred grove of Andhra Pradesh, India. J Pharm Sci Res 2014;6:382-8.

Maitreya BB. An overview of ethnomedicinal plants of family rubiaceae from sabarmati river of Gujarat state, India. Int J Pharm Life Sci 2015;6:4476-80.

Shiddamallayya N, Rao RV, Venkateshwarlu G, Giri SK, Doddamani SH. Traditional local health practices of Haveri, Karnataka. Unique J Ayurvedic Herbal Med 2015;3:135-45.

Sridhar SK, Ramachandran S, Anbalagan N, Leonard TJ, Joanofarc J, Kumar SS. Pharmacological screening of dikamali resin extract. Nat Prod Sci 2003;9:10-2.

Sabbani PK, Chityala PK, Gowrishankar NL, Kumar NG, Shilpa K, Tejaswi CH. Evaluation of antioxidant and antiulcer activity of methanolic extract of Gardenia gummifera L. in rats. Asian J Pharm Clin Res 2015;8:41-4.

Vindhya K, Leelavathi S. Evaluation of antioxidant properties and total phenolic content of Gardenia gummifera Linn. Int J Pharm Sci Rev Res 2015;32:255-61.

Narware N, Mishra PK, Shrivastava PN. Preliminary phytochemical study and antimicrobial activity of the gum extracts of Gardenia gummifera Linn. Int J Green Herb Chem 2014;3:717-21.

Suryadeva P, Khanam S. Screening of plant extracts for larvicidal activity against Culex quinquefasciatus. J Nat Rem 2002;2:186-8.

Vindhya K, Leelavathi S. In vitro cytotoxic activities of leaf extract of Gardenia latifolia Ait. and Gardenia gummifera Linn. Int J Pharm Sci Res 2014;5:4975-8.

Sabbani PK, Thatipelli RC, Surampalli G, Duvvala P. Evaluation of hepatoprotective activity with different fractions of Gardenia gummifera Linn. on paracetamol induced liver damage in rats. J Drug Metab Toxicol 2016;7:1.

Pal CR, Garge VN, Kadam VJ. Antihyperlipidemic activity of Gardenia gummifera. J Med Sci Clin Res 2015;3:5000-10.

Prabha SP, Ansil PN, Nitha A, Wills PJ, Latha MS. Anti-atherogenic activity of methanolic extract of Gardenia gummifera Linn. F. on high fat diet induced atherosclerosis in rats. Int J Pharm Pharm Sci 2013;5:388-93.

Prabha SP, Nitha A, Ansil PN, Latha MS. Cardioprotective effect of methanolic extract of Gardenia gummifera Linn. F. on isoproterenol induced myocardial infarction in rats. Int J Pharm Sci Res 2014;5:3817-28.

Kekuda PTR, Raghavendra HL, Solomon T, Duressa D. Antifungal and antiradical potential of Moringa stenopetala (Baker f.) Cufod (Moringaceae). J Biosci Agric Res 2016;11:923-9.

Raghavendra HL, Kekuda PTR, Vijayananda BN, Duressa D, Solomon T. Nutritive composition and antimicrobial activity of Moringa stenopetala (Baker f.) Cufod. J Adv Med Pharm Sci 2016;10:1-9.

Kaushik R, Saini P. Screening of some semi-arid region plants for larvicidal activity against Aedes aegypti mosquitoes. J Vector Borne Dis 2009;46:244–6.

Selvaraj M, Mosses M. Efficacy of melia azedarach on the larvae of three mosquito species Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae). Eur Mosquito Bull 2011;29:116-21.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.

Sibanda T, Okoh AI. The challenges of overcoming antibiotic resistance: Plant extracts as potential sources of antimicrobial and resistance modifying agents. Afr J Biotechnol 2007;6:2886-96.

Abdullah EM. Plants: an alternative source for antimicrobials. J Appl Pharm Sci 2011;1:16-20.

Kekuda PTR, Rakesh KN, Dileep N, Junaid S, Pavithra GM, Gunaga SS, et al. Antimicrobial and antioxidant activity of Anaphalis lawii (Hook. f.) Gamble. Sci Technol Arts Res J 2012;1:8-16.

Al-Mariri A, Safi M. In vitro antibacterial activity of several plant extracts and oils against some gram-negative bacteria. Iranian J Med Sci 2014;39:36-43.

Kekuda PTR, Siddiqha A, Pushpavathi D, Vinayaka KS, Raghavendra HL. Radical scavenging, cytotoxic and antimicrobial activity of Flacourtia indica (Burm. f.) Merr. Med Health Sci Res J 2017;1:76-82.

Fankam AG, Kuiate JR, Kuete V. Antibacterial and antibiotic resistance modulatory activities of leaves and bark extracts of Recinodindron heudelotii (Euphorbiaceae) against multidrug-resistant gram-negative bacteria. BMC Complementary Altern Med 2017;17:168.

Wanjiru KE, Mwangi EM, Cheplogoi PK. Screening for antimicrobial compounds in Gardenia volkensii fruits (Rubiaceae). Int J Sci Eng Res 2013;4:1253-6.

Njinga NS, Sule MI, Pateh UU, Hassan HS, Usman MA, Bilkisu A, et al. Phytochemical and antimicrobial activity of the stem-bark of Gardenia aqualla Stapf and Hutch (Rubeacea). J Med Plant Res 2014;8:942-6.

Gaurea SH, Bapat UC. Study of antibacterial activity of resins of Boswellia serrata Roxb Ex Colebr., Commiphora mukul (Hooks Ex-stocks) Engl, Gardenia resinifera Roth. and Shorea robusta Gaertn. Int J Pharm Pharm Sci 2016;8(S1):29-31.

Yazdani D, Tan YH, Abidin ZMA, Jaganath IB. A review on bioactive compounds isolated from plants against plant pathogenic fungi. J Med Plants Res 2011;5:6584-9.

Rongai D, Milano E, Sciò E. Inhibitory effect of plant extracts on conidial germination of the phytopathogenic fungus Fusarium oxysporum. Am J Plant Sci 2012;3:1693-8.

Gaikwad RS, Kakde RB, Kulkarni AU, Gaikwad DR, Panchal VH. In vitro antimicrobial activity of crude extracts of Jatropha species. Curr Bot 2012;3:9-15.

Masangwa JIG, Aveling TAS, Kritzinger Q. Screening of plant extracts for antifungal activities against Colletotrichum species of common bean (Phaseolus vulgaris L.) and cowpea (Vigna unguiculata (L.) Walp). J Agric Sci 2013;151:482–91.

Chandra M, Mahesh NM. Antifungal activity of medicinal plant extracts against seed-borne pathogenic fungi. Acta Biologica Indica 2013;2:481-3.

Parveen S, Wani AH, Ganie AA, Pala SA, Mir RA. Antifungal activity of some plant extracts on some pathogenic fungi. Arch Phytopathol Plant Prot 2014;47:279-84.

Yazgi M, Awad D, Jreikous B. Screening of the antifungal activity of plant Mentha longifolia crude extracts against two fungi Alternaria citri and Fusarium moniliforme. J Entomol Zool Stud 2015;3:359-64.

Kafua L, Kritzinger Q, Hussein AA. Antifungal activity of Gardenia brighamii leaf extracts. South Afr J Bot 2010;76:411.

Lelono RA, Tachibana S, Itoh K. Isolation of antifungal compounds from Gardenia jasminoides. Pak J Biol Sci 2009;12:949-56.

Chatterjee S, Poduval TB, Tilak JC, Devasagayam TPA. A modified, economic, sensitive method for measuring total antioxidant capacities of human plasma and natural compounds using Indian saffron (Crocus sativus). Clin Chim Acta 2005;352:155-63.

Shirwaikar A, Prabhu KS, Punitha ISR. In vitro antioxidant studies of Sphaeranthus indicus (Linn). Indian J Exp Biol 2006;44:993-6.

Bhutia RD, Upadhyay B, Maneesh M. Association of plasma level of thiobarbituric acid reactive substances with extent of hepatocellular injury in preterm infants with cholestatic jaundice. Indian J Clin Biochem 2006;21:39-41.

Choi Y, Jeong H, Lee J. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 2007;103:130-8.

Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complementary Altern Med 2008;8:63.

Debnath T, Park P, DebNath NC, Samad NB, Park HW, Lim BO. Antioxidant activity of Gardenia jasminoides Ellis fruit extracts. Food Chem 2011;128:697-703.

Uddin R, Saha MR, Subhan N, Hossain H, Jahan IA, Akter R, et al. HPLC-analysis of polyphenolic compounds in Gardenia jasminoides and determination of antioxidant activity by using free radical scavenging assays. Adv Pharm Bull 2014;4:273–81.

Sayari N, Saidi MN, Sila A, Ellourz-chaabouni S, Bougatef A. Chemical composition, angiotensin I-converting enzyme (ACE) inhibitory, antioxydant and antimicrobial activities of Ononis natrix leaves extracts. Free Radicals Antioxid 2016;6:23-33.

Urquiza-Martínez MV, Navarro BF. Antioxidant capacity of food. Free Radicals Antioxid 2016;6:1-12.

Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26:211-9.

Chung Y, Chien C, Teng K, Chou S. Antioxidative and mutagenic properties of Zanthoxylum ailanthoides Sieb and zucc. Food Chem 2006;97:418-25.

Krishna MS, Nair JA. Antibacterial, cytotoxic and antioxidant potential of different extracts from leaf, bark and wood of Tectona grandis. Int J Pharm Sci Drug Res 2010;2:155-8.

Menon DB, Sasikumar JM, Latha K. Phytochemical analysis and antioxidant activity of methanolic extract of Plectranthus hadiensis (Forssk.) Schweinf. ex Spreng. aerial parts. Indian J Nat Prod Resour 2012;3:359-65.

Pazhanisamy M, Ebenezer GAI. Antioxidant activity of leaves of an important medicinal plant Ormocarpum cochinchinense (Lour.) Merr. J Modern Biotechnol 2013;2:89-94.

Fidrianny I, Rahmiyani I, Wirasutisna KR. Antioxidant capacities from various leaves extracts of four varieties mangoes using DPPH, ABTS assays and correlation with total phenolic, flavonoid, carotenoid. Int J Pharm Pharm Sci 2013;5:189-94.

Shalaby EA, Shanab SMM. Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian J Geo-Mar Sci 2013;42:556-64.

Ajileye OO, Obuotor EM, Akinkunmi EO, Aderogba MA. Isolation and characterization of antioxidant and antimicrobial compounds from Anacardium occidentale L. (Anacardiaceae) leaf extract. J King Saud Univ Sci 2015;27:244-52.

Vindhya K, Kumar PPM, Roopa AR, Leelavathi S. Evaluation of in vitro antioxidant potential of various leaf extracts of Gardenia latifolia Ait. Int J Innovative Pharm Sci Res 2015;3:176-90.

Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal 2006;19:669-75.

Rajurkar NS, Hande SM. Estimation of phytochemical content and antioxidant activity of some selected traditional Indian medicinal plants. Indian J Pharm Sci 2011;73:146-51.

Fan Y, Ge Z, Luo A. In vitro antioxidant activity of polysaccharide from Gardenia jasminoides Ellis. J Med Plants Res 2011;5:2963-8.

Katyal R, Tewari P, Rahman SJ, Pajni HR, Kumar K, Gill KS. Susceptibility status of immature and adult stages of Aedes aegypti against conventional insecticides in Delhi, India. Dengue Bull 2001;25:84-7.

Promsiri S, Naksathit A, Kruatrachue M, Thavara U. Evaluations of larvicidal activity of medicinal plant extracts to Aedes aegypti (Diptera: Culicidae) and other effects on a non target fish. Insect Sci 2006;13:179-88.

Elimam AM, Elmalik KH, Ali FS. Efficacy of leaves extract of Calotropis procera Ait. (Asclepiadaceae) in controlling Anopheles arabiensis and Culex quinquefasciatus mosquitoes. Saudi J Biol Sci 2009;16:95-100.

Phukerd U, Soonwera M. Larvicidal and pupicidal activities of essential oils from Zingiberaceae plants against Aedes aegypti (Linn.) and Culex quinquefasciatus say mosquitoes. Southeast Asian J Trop Med Pub Health 2013;44:761-71.

Panneerselvam C, Murugan K, Kovendan K, Kumar PM, Subramaniam J. Mosquito larvicidal and pupicidal activity of Euphorbia hirta Linn. (Family: Euphorbiaceae) and Bacillus sphaericus against Anopheles stephensi Liston. (Diptera: Culicidae). Asian Pac J Trop Med 2013;6:102-9.

Valentina J, Poonguzhali TV, Nisha JLLL. Mosquito larvicidal and pupicidal activity of seaweed extracts against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus. Int J Mosquito Res 2015;2:54-9.

Nwankwo EN, Okonkwo NJ, Ogbonna CU, Akpom CJO, Egbuche CM, Ukonze BC. Moringa oleifera and Annona muricata seed oil extracts as biopesticides against the second and fourth larval instar of Aedes aegypti L. (Diptera: Culicidae). J Biopestic 2015;8:56-61.

El-Bokl MM. Toxicity and bioefficacy of selected plant extracts against the mosquito vector Culex pipiens L. (Diptera: Culicidae). J Entomol Zool Stud 2016;4:483-8.



How to Cite

T. R., P. K., R. H. L., S. M., P. D., T. Petkar, and A. Siddiqha. “ANTIMICROBIAL, ANTIRADICAL AND INSECTICIDAL ACTIVITY OF GARDENIA GUMMIFERA L. F. (RUBIACEAE)”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 10, Oct. 2017, pp. 265-72, doi:10.22159/ijpps.2017v9i10.20252.



Original Article(s)