IN VIVO ANTICANCER EVALUATION OF (2-AMINO-3,4,5-TRIMETHOXYPHENYL) (6-METHOXY-1H-INDOL-3-YL) METHANONE


Vijay K. Patel, Harish Rajak

Abstract


Objective: The design and development of Combretastatin A-4 analogues as anticancer agent has always attracted the attention of scientific community involved in anticancer research. The aim of the present work was to investigate the in-vivo cytotoxic activity of a novel CA-4 analogues i.e., (2-amino-3,4,5-trimethoxyphenyl)(6-methoxy-1H-indol-3-yl)methanone.

Method: The in-vivo cytotoxic activity of test compound was examined using Human Tumor Xenograft model on MCF-7 cancer cell line in Balb/c mice.

Result: The test compound exhibited excellent cytotoxic activity against MCF-7 (0.013 µM), and colon HT-29 (0.143 µM), slightly higher than CA-4 activity. Relative Tumor Volume (RTV) value was found to increase rapidly in control group A during the period of 1 to 18 days indicating the higher growth rates of tumor. On the other hand group B and C showed slower growth rate of tumor as a result of treatment with CA-4 and test compound, respectively. The group B treated with CA-4 (5 mg/kg) showed significant inhibition in tumor growth during day 5 to 18 with %TGI in range of 47.24%-62.31% as compared to control group. The group C treated with test compound  (5mg/kg) caused significant inhibition in tumor growth during day 5 to 18 with %TGI in range of 55.66%-75.93% as compared to control group. The % survival value was found 100% indicating that CA-4 and test compound were nontoxic at the dose of 5mg/kg under experimental conditions.

Conclusion: The parameters of In-vivo anticancer evaluation i.e., relative tumour volume and % tumor growth inhibition showed better anticancer potential of test compound. It was further supported by non-toxic nature of test compound as indicated by 100% survival value determined at the dose of 5 mg/kg during in-vivo studies.

Keywords


Combretastatin A-4, in-vivo, Human Tumor Xenograft model, Aroylindole derivative.

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About this article

Title

IN VIVO ANTICANCER EVALUATION OF (2-AMINO-3,4,5-TRIMETHOXYPHENYL) (6-METHOXY-1H-INDOL-3-YL) METHANONE

Keywords

Combretastatin A-4, in-vivo, Human Tumor Xenograft model, Aroylindole derivative.

DOI

10.22159/ijpps.2017v9i11.20439

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 11, 2017 Page: 45-49

Online ISSN

0975-1491

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39 Views | 44 Downloads

Authors & Affiliations

Vijay K. Patel
Medicinal Chemistry Research Laboratory, SLT Institute of Pharmaceutical Sciences, Guru Ghasidas University, Bilaspur 495009, (C.G.) India
India

Harish Rajak
Medicinal Chemistry Research Laboratory, SLT Institute of Pharmaceutical Sciences, Guru Ghasidas University, Bilaspur 495009, (C. G.) India
India


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