ISOLATION OF 2-CHLOROBENZIMIDAZOLE FROM MELIA DUBIA LEAF EXTRACT AND ITS STRUCTURAL CHARACTERISATION
Objective: Natural products from medicinal plants, either as isolated compounds or as standardized plant extracts exhibit promising source of medicinal activity against various diseases. The aim of the present work was to make an attempt of isolation of bioactive principle and characterization of the isolated compound, from the medicinal plant Melia dubai
Methods: The extraction was done by a cold percolation method and the compound was separated and isolated by chromatography technique such as a thin layer chromatography (TLC), column chromatography and high-performance liquid chromatography (HPLC). The isolated compound was crystallized and the structural characterization of the isolated compound was made using UV-Visible, FT-IR, 1H-NMR, GC-MS and MS techniques which confirmed the structure of the isolated compound.
Results: The separated and isolated compound was characterized by both physical and spectral methods like Ultraviolet-Visible spectroscopy (UV-Visible), Fourier transform infrared spectroscopy (FT-IR), Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), Gas chromatography-mass spectrometry (GC-MS), and Mass spectrometry(MS). Based on the studies, organizational characteristics of one bioactive principle were deciphered. The results revealed that the isolated species is 2-chlorobenzimidazole and it agreed well with the reported value and spectra for 2-chlorobenzimidazole.
Conclusion: The above results obtained in this research work clearly indicated the promising occurrence of 2-chlorobenzimidazole in Media dubia plant leaves. The future scope of these studies may guide us to view the biological activity of the isolated compound.
2. Gopal V, Prakash Yoganandam G, Manju P. Concise review on Melia Dubia cav. (meliaceae). Eur J Environ Ecol 2015;2:57-60.
3. Dayana Jeyaleela G, Irudaya Monisha S, Anitha Immaculate A, Rosaline Vimala J. Studies on phytochemical, nutritional analysis and screening of in vitro biological activities of Melia Dubia leaf extract. Int J Sci Eng Res 2016;7:56-68.
4. Murugesan S, Senthilkumar N, Rajeshkannan C, Vijayalakshmi KB. Phytochemical characterization of Melia Dubia for their biological properties. Chem Sin 2013;4:36-40.
5. Raman N. Phytochemical Techniques. New India Publishing Agency; 2006. p. 275-86.
6. Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plants extracts. Afr J Tradit Complementary Altern Med 2011;8:1-10.
7. Harborne JB. A guide to modern techniques of plant analysis. USA: Kluwer Academic Publisher; 1998. p. 14-5.
8. Pfoze NL, B Myrboh B, Kumar Y, Rohman R. Isolation of protoberberine alkaloids from stem bark of Mahonia Manipurensis Takeda using RP-HPLC. J Med Plants Studies 2014;2:48-57.
9. Veena Sharma, Manu Singh. Isolation and characterization of stigma-5,22dien-3-O-b-D-glucopyranoside from the ethanolic root extract of Operculina Turpethum. Int J Adv Res 2013;1:303-12.
10. Pratap Chandran R, Dibu Divakaran, Consortium OSDD. Isolation and characterization of antimycobacterial compounds from the leaf of Aloe vera (L.) Burm. J Appl Pharm Sci 2017;7:217-22.
11. Veena Sharma, Ritu Paliwal. Isolation and characterization of saponins from Moringa Oleifera (moringaeceae) pods. Int J Pharm Pharm Sci 2013;5:179-83.
12. Sajjad Pasha, Salma Khanam, Zeeshan Afsar. Isolation and characterization of chemical constituents of Asparagus Racemosus as markers. Int J Res Dev Pharm L Sci 2016;5:2255-63.
13. Henok Gulilat Azalework, Sahabjada, Asif jafri, MD Arshad, Tabarak Malik. Phytochemical investigation, GC-MS profile and antimicrobial activity of a medicinal plant Ruta graveolens l. from ethiopia. Int J Pharm Pharm Sci 2017;9:29-34.
14. http://webbook.nist.gov/cgi/inchi?ID=C4857061andMask=80. [Last accessed on 05 May 2017]
15. http://webbook.nist.gov/cgi/inchi?ID=C4857061andMask=200. [Last accessed on 05 May 2017]