INVESTIGATION OF EFFECT OF PHOSPHOLIPIDS ON PHYSICAL AND FUNCTIONAL CHARACTERIZATION OF PACLITAXEL LIPOSOMES


Amol A. Tatode, Arun T. Patil, Milind J. Umekar, Darshan R. Telange

Abstract


Objective: Aim of the present investigation was to determine the effect of various synthetic grades of phospholipids on paclitaxel liposomes (PTL).

Methods: The PTL formulations using various grades of phospholipids were prepared by film hydration method. The prepared PTL formulations were physicochemically characterized by entrapment efficiency (EE, %w/w), vesicular size and particle size distribution. These formulations were also characterized for function parameters such as in vitro release and hemolytic toxicity assay.

Results: The synthetic grades of phospholipids significantly influenced PTL formulations. The stoichiometric ratio (1:1) between CH and various synthetic phospholipids was found to be optimized one, from rest of the ratios. The characterization confirmed the formation of PTL. The EE was observed to be high (86.67%) as increasing the ratios between CH and phospholipids but then declined suddenly as further increasing the ratio. The best liposomal formulations showed that the spherical shape was found to be within size ranging from<10 µm, with a higher rate and extent of the release, ~86.22% of paclitaxel from PTL formulation. The results of the hemolytic toxicity study demonstrated that PTL formulations with a ratio (1:1) exhibited a significantly lower hemolytic toxicity (2.70%), compared to all formulations.

Conclusion: The result revealed the excellent effect of phospholipids on paclitaxel liposomes. The paclitaxel liposomes prepared with CH: PL90G ratio (1:1) was found to be optimized one. The entrapment efficiency, particle size distribution, in vitro release and hemolytic activity with this ratio shown to be excellent as compared to other ratios.


Keywords


Liposomes, Phospholipon, Entrapment efficiency, In vitro release, Hemolytic toxicity

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About this article

Title

INVESTIGATION OF EFFECT OF PHOSPHOLIPIDS ON PHYSICAL AND FUNCTIONAL CHARACTERIZATION OF PACLITAXEL LIPOSOMES

Keywords

Liposomes, Phospholipon, Entrapment efficiency, In vitro release, Hemolytic toxicity

DOI

10.22159/ijpps.2017v9i12.20749

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 12, 2017 Page: 141-146

Online ISSN

0975-1491

Statistics

22 Views | 10 Downloads

Authors & Affiliations

Amol A. Tatode
Department of Pharmaceutics, University Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra 440033, India
India

Arun T. Patil
Smt. Kishoritai Bhoyar College of Pharmacy, Kamptee, Nagpur, Maharashtra – 441002, India.

Milind J. Umekar
Smt. Kishoritai Bhoyar College of Pharmacy, Kamptee, Nagpur, Maharashtra – 441002, India

Darshan R. Telange
Smt. Kishoritai Bhoyar College of Pharmacy, Kamptee, Nagpur, Maharashtra – 441002, India


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