PRODUCTION AND PARTIAL CHARACTERIZATION OF PIGMENTS PRODUCED BY KOCURIA SP BRI 36: INFLUENCE OF HEAVY METALS
Objective: To study the production of pigments by Kocuria sp. BRI 36, their characteristics and influence of heavy metals on pigments.
Methods: The effects of various physical and chemical parameters on pigments production by Kocuria sp. BRI 36 were examined. Pigments were extracted and partially characterised by Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectroscopy (FTIR). The effects of heavy metals such as Pb2+, Cd 2+, Ni2+ and Cr3+ were studied on pigment production. Antimicrobial activity and stability studies of crude pigment were also conducted.
Results: Kocuria sp. BRI 36 isolated from cold oceanic region maximally produced red-orange pigment in presence of glucose (5% w/v) and protease peptone (0.2% w/v) at pH 7.5, 10Â±1 Â°C. Thin layer chromatography (TLC) analysis revealed the occurrence of three different compounds in the crude pigment belonging to carotenoid and xanthophyll group. Metals like Ni2+ and Cr3+ adversely affected pigment production while Pb2+and Cd2+enhanced the yield. The significant features of Kocuria sp. BRI 36 pigment are i) antimicrobial activity against Gram-positive and Gram-negative bacteria, ii) maximum stability at pH 7.5 and 10Â±1 Â°C and iii) ~38% color loss at 50Â±1 Â°C in 5 h.
Conclusion: Our results suggest application potential of Kocuria sp. BRI 36 pigments in various biotechnological fields.
2. Vishnu TS, Palaniswamy M. Isolation and identification of Chromobacterium sp. from different ecosystems. Asian J Pharm Clin Res 2016;9:253-7.
3. Kulkarni VM, Gangawaneb DP, Patwardhana AV, Adivarekarb RV. Dyeing of silk/wool using crude pigment extract from an isolate Kocuria flava sp. Ho-9041. J Environ Res 2014;2:314-20.
4. Alihosseini F, Ju KS, Lango J, Hammock BD, Sun G. Antibacterial colorants: characterization of prodiginines and their applications on textile materials. Biotechnol Prog 2008;24:742â€“7.
5. Ahmad AS, Ahmad WYW, Zakaria ZK, Yosof NZ. Applications of bacterial pigments as a colorant. The Malaysian perspective. 1st edition. Verlag Berlin Heidelberg: Springer; 2008.
6. Alcantara S, Sanchez S. Influence of carbon and nitrogen sources on Flavobacterium growth and zeaxanthin biosynthesis. J Indian Microbiol Biotechnol 1999;23:697â€“700.
7. Lorquin J, Moluba F, Drefus BL. Identification of carotenoid pigment canthaxanthin from photosynthetic Bradyrhizobium strains. Appl Environ Microbiol 1997;53:1151-4.
8. Stackebrandt E, Koch C, Gvozdiak O, Schumann P. Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov, Nesterenkonia gen. nov, Kytococcus gen. nov, Dermacoccus gen. nov, and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 1995;45:682â€“92.
9. Yusef HH, Belal AM, El-Sharouny EE. Production of natural pigments from novel local psychrotolerant Kocuria spp. Life Sci J 2014;11:500-7.
10. Pote S, Chaudhary Y, Upadhayay S, Tale V, Walujkar S, Bhadekar R. Identification and biotechnological potential of psychrotrophic marine isolates. Eurasia J Biosci 2014;8:51-60.
11. Durve A, Naphade S, Bhot M, Varghese J, Chandra N. Quantitative evaluation of heavy metal bioaccumulation by microbes. J Microbiol Biotechnol Res 2013;3:21-32.
12. Mulik AR, Bhadekar RK. Heavy metal removal by bacterial isolates from the Antarctic oceanic region. Int J Pharm Biol Sci 2017;8:535-43.
13. GudiÃ±a EJ, Rocha V, Teixeira JA, Rodrigues LR. Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20. Lett Appl Microbiol 2010;50:419â€“24.
14. Vora JU, Jain NK, Modi AH. Identification and characterization of pigment producing strain Kocuria KM243757 and JO1KM216829 from Kharaghoda soil. Int J Curr Microbiol Appl Sci 2015;4:850-9.
15. Lorenz RT. HPLC and spectrophotometric analysis of carotenoids from Haematococcus algae. BioAstin/NaturoseTM Technical Bulletin No. 20 Kailua-Kona Hawai: Cvanotech Corporation; 2001.
16. Kar JR, Hallsworth JE, Singhal RS. Fermentative production of glycine betaine and trehalose from acid whey using Actinopolyspora halophila (MTCC 263). Environ Technol Innov 2015;3:68-76.
17. Stevenson A, Hamill PG, Dijksterhuis J, Hallsworth JE. Water-, pH-and temperature relations of germination for the extreme xerophiles Xeromyces bisporus (FRR 0025), Aspergillus penicillioides (JH06THJ) and Eurotium halophilicum (FRR 2471). Microbial Biotechnol 2016;10:330-40.
18. Jadhav VV, Yadav Y, Shouche Y, Bhadekar RK. Isolation and cellular fatty acid composition of psychrotrophic Halomonas strains from cold sea water. Songklanakarin J Technol 2013;35:287-92.
19. Medicharla V, Jagannadham V, Rao J, Shivaji S. The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain: purification, structure and interaction with synthetic membranes. J Bacteriol 1991;173:7911-7.
20. Kumar A, Vishwakarma SH, Singh J. Microbial pigments: production and their applications in various industries. J Pharm Chem Biol Sci 2015;5:203-12.
21. Kim YS, Park JS. Characterization of pigment-producing Kocuria sp. K70 and the optimal conditions for pigment production and physical stability. KSBB J 2010;25:513-9.
22. El-Sharouny EE, Belal MA, Yusef HH. Isolation and characterization of two novel local psychrotolerant Kocuria spp. with high affinity towards metal cations biosorption. Life Sci J 2013;10:1721-37.
23. Subhasree RS, Babu DP, Vidyalakshmi R, Mohan CV. Effect of carbon and nitrogen sources on stimulation of pigment production by Monascus purpureus on jackfruit seeds. Int J Microbiol Res 2011;2:184-7.
24. Jonathan AC, Andrew NWB, Prashanth B, Allen YM, David JT, Hallsworth JE. The biology of habitat dominance; can microbes behave as weeds? J Microbiol Biotechnol 2013;6:453-92.
25. Oren A, Hallsworth JE. Microbial weeds in hypersaline habitats: the enigma of the weed-like Haloferax mediterranei. FEMS Microbiol Lett 2014;359:134-42.
26. Jeong DW, Park JS. Characterization of pigment-producing Pseudoalteromonas spp. from marine habitats and their optimal conditions for pigment production. J Life Sci 2008;18:1752-7.
27. Flavia LA, Andrew S, Esther B, Jenny LMG, Fakhrossadat H, Sandra H, et al. Concomitant osmotic and chaotropicity-induced stresses in Aspergillus wentii: compatible solutes determine the biotic window. Curr Genet 2015;61:457-77.
28. Andrew S, Jonathan AC, Jim PW, Ricardo S. Is there a common water-activity limit for the three domains of life? ISME J 2015;9:1333-51.
29. Wong LS, Teo SC. Naturally occurring carotenoids in cyanobacteria as a bioindicator for heavy metals detection. Proc. of the Intl. Conf. on Advances. In: Applied Science and Environmental Engineeringâ€“ASEE; 2014.
30. Priyalaxmi R, Murugan A, Paul R, Raj DK. Bioremediation of cadmium by Bacillus safensis (JX126862), a marine bacterium isolated from mangrove sediments. Int J Curr Microbiol Appl Sci 2014;3:326-35.
31. Hussein K, Abdul-Sada. A resistance study of Pseudomonas aeruginosa to heavy metals. Bas J Vet Res 2009;8:52-60.
32. Silva ALD, Carvalho MARD, De Souza SAL, Dias PMT. Heavy metal tolerance (Cr, Ag AND Hg) in bacteria isolated from sewage. Braz J Microbiol 2012;43:1620â€“31.
33. Lin TF, Demain AL. Resting cell studies on the formation of water-soluble red pigments by Monascus sp. J Ind Microbiol 1993;12:361â€“70.
34. Shatila F, Yusef H, Holail H. Pigment production by Exiguobacterium aurantiacum FH, a novel Lebanese strain. Int J Curr Microbiol Appl Sci 2013;2:176-91.
35. Kushwaha K, Saxena J, Agarwal KM. Antibacterial activity by pigmented psychrotrophic bacterial isolates. Indian J Appl Res 2014;4:168-74.
36. Mohana Srinivasan V, Sriram Kalyan P, Nandi I, Subathradevi C, Selvarajan E, Suganthi V, et al. Fermentative production of extracellular pigment from Streptomyces coelicolor MSIS1. Res J Biotech 2013;8:31-41.
37. Reddy SN, Jogadhenu SS, Prabahar PV, Matsumoto GI. Kocuria Polaris sp. nov., an orange pigmented psychrophilic bacterium isolated from an cold cyanobacterial mat sample. Int J Syst Evol Microbiol 2003;53:183-7.
38. Latha BV, Jeevaratnam K. Purification and characterization of the pigments from Rhodotorula glutinis DFR-PDY isolated from a natural source. Global J Biotech Biochem 2010;5:166-74.
39. Yuan L, Koehler M, Baudelet M, Richardson M. Fusion of infrared and Raman spectroscopy for carotenoid analysis. Pittcon Orlando FL USA 2012;3:1-13.
40. Suryawanshi RK, Patil CD, Borase HP, Narkhede CP, Stevenson A, Hallsworth JE, et al. Towards an understanding of bacterial metabolites prodigiosin and violacein and their potential for use in commercial sunscreens. Int J Cosmet Sci 2015;37:98-107.