Rima Thiyam, Mangamoori Lakshmi Narasu


Objective: The objective of the present study was to investigate the growth inhibitory effect, apoptosis initiation and genotoxic effect of zerumbone (ZER), a phytochemical and cisplatin, a chemotherapeutic drug on human colorectal cancer cell line COLO205 and normal human lymphocytes.

Methods: The antiproliferative activity of ZER and cisplatin (positive control) on COLO205 cells and lymphocytes was analysed by 3( 4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) assay. Morphological analysis of the cells was studied by using inverted phase contrast microscope. Propidium iodide staining method was used to observe the apoptotic morphological changes in the treated cells. Finally comet assay was conducted to observe the extent of DNA damage induced by ZER and cisplatin on COLO205 and lymphocytes.

Results: ZER and cisplatin exhibited growth inhibition in a dose and time dependent manner against COLO205 with no considerable effect on lymphocytes. The IC50 values of ZER on COLO205 for 24h, 48h and 72h were 19 µg/ml, 10 µg/ml and 5 µg/ml. Comparatively the IC50 values of cisplatin on COLO205 for 24h, 48h and 72h were 38 µg/ml, 24 µg/ml and 15 µg/ml.  Morphological changes such as cell shrinkage, membrane blebbing and nuclear condensation were observed in COLO205 while no significant change was observed in lymphocytes. Fluorescence imaging studies confirmed apoptotic cell death in treated COLO205 cells while no significant cell death was observed in treated lymphocytes. Comet assay revealed significant DNA damage in treated COLO205 cells.

Conclusion: The present study demonstrated the cytotoxic and genotoxic effect of ZER and cisplatin on COLO205 cells. These drugs showed no significant effect on lymphocytes.


Zerumbone, Cisplatin, COLO205, Lymphocytes, MTT assay, Comet assay

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Rates SMK. Plants as source of drugs. Toxicon2011;39:603–13.

Charu G,Prakash D. Phytonutrients as therapeutic agents. J Complementary Integr Med 2014;11:151–69.

Murakami A, Takahashi D, Kinoshita T, Koshimizu K, Kim HW, Yoshihiro A,et al.Zerumbone, a Southeast Asian ginger sesquiterpene, markedly suppress free radical generation, proinflammatory protein production, and cancer cell proliferation accompanied by apoptosis: the α, the β-unsaturated carbonyl group is a prerequisite.Carcinogenesis2002;23:795-802.

Kitayama T, Okamoto T, Hill RK, Kawai Y, Takahashi S, Yonemori S,et al. Chemistry ofzerumbone. 1. simplified isolation, conjugate addition reactions, and a unique ring contracting transannular reaction of its dibromide. J Org Chem 1999;64:2667–72.

Zakaria ZA, Mohamad AS, Chear CT, Wong YY, Israf DA,Sulaiman MR.Antiinflammatory and antinociceptive activities of Zingiberzerumbetmethanol extract in experimental model systems.Med PrincPract2010;19:287–94.

Habsah M, Amran M, Mackeen MM, Lajis NH, kikuzaki H, NakatanI N,et al.Screening of Zingiberaceae extracts for antimicrobial and antioxidant activities. J Ethnopharmacol2000;72:403–10.

Chandra K, Graeme HM, Ian RR, Graham PJ. Antitumor activity of anextract of Zingiberaromaticum and its bioactive sesquiterpenoidZerumbone. Nutr Cancer 2003;45:218-25.

Swagatika D, Monalisa R, Abtar M, Sajad S, Gopinath AK, Sanghamitra N, et al.Zerumbone, a natural plant dietary compound induces expression of interleukin-12P70 cytokine in human peripheral blood mononuclear cells. Asian J Pharm Clin Res 2016;9Suppl3:312-5.

Sharifah Sakinah SA, Handayani ST,Azimahtol Hawariah LP.Zerumbone induced apoptosis in liver cancer cells via modulation of Bax/Bcl-2 ratio. Cancer Cell Int 2007;7:4.

Supachai Y, Bokyung S, Pornngarm L, Bharat B. Zerumbone enhances TRAIL-induced apoptosis through the induction of death receptors in human colon cancer cells: evidence for an essential role of reactive oxygen species. Cancer Res2009;69:6581-9.

Rahman HS, Rasedee A, How CW, Abdul AB, Zeenathul NA, Othman HH, et al.Zerumbone-loaded nanostructured lipid carriers: preparation, characterization, and antileukemic effect. Int J Nanomed 2013;8:2769-81.

Celik M, Unal F, Yuzbasioglu D, Ergun MA, Arslan O, Kasap R. In vitro effect of karathane LC (dinocap) on human lymphocytes. Mutagenesis2005;20:101–4.

Duthei SJ, Perie L, JenkinsonAMcE, Narayanan S. Cryopreserved versus freshly isolated lymphocytes in human biomonitoring: endogenous and induced DNA damage,antioxidant status and repair capability. Mutagenesis 2002;17:211-4.

Houda S, Hayat T, Ibtissam T, Souad A, Abderraouf H. Evaluation of the genotoxicity of essential oil from OriganumcompactumBenth. in human lymphocytes. Asian J Pharm Clin Res 2016;9Suppl2:274-6.

Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H,et al.Single-cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen2000;35:206-21.

Murakami A, Tanaka T, Lee JY, Surh YJ, Kim HW, Kawabata K, et al. ZER, a sesquiterpene in subtropical ginger, suppresses skin tumor initiation and promotion stages in ICR mice. Int J Cancer 2004;110:481–90.

Shukla Y, Singh M. Cancer preventive properties of ginger: a brief review. Food ChemToxicol2007;45:683–90.

Hoffman A, Spetner LM, Burke M. Redox-regulated mechanism may account for zerumbone’s ability to suppress cancer-cell proliferation. Carcinogenesis 2002;23:1961.

O’Brien MA, Kirby R. Apoptosis: a review of pro-apoptotic and anti-apoptotic pathways and dysregulation in disease. J Vet EmergCrit Care 2008;18:572–85.

Singh RK, Mishra SK, Kumar N, Singh AK. Assessment of DNA damage by cometassay in lymphocytes of workers occupationally exposed to petroleum fumes. Int J Genet2010;2:18-22.

Adel SA, Ahmad BA, Syam MM. Evaluation of the genotoxicity of zerumbone in cultured human peripheral blood lymphocytes. ToxicolIn Vitro2010;24:707-12.

Adel SA. Genotoxicity assessment of a natural anti-cancer compound zerumbone on CHO cell lines. Int J Cancer Res 2012;8:119-29.

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Zerumbone, Cisplatin, COLO205, Lymphocytes, MTT assay, Comet assay





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International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 11, 2017 Page: 92-96

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Authors & Affiliations

Rima Thiyam
Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad 500085, Telangana, India

Mangamoori Lakshmi Narasu
Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad 500085, Telangana, India

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