• Leenawaty Limantara Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia
  • Renny Indrawati Chemistry Study Program, Faculty of Sains and Technology, Universitas Ma Chung, Malang, Indonesia
  • Dian Ekawati Wijaya Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia
  • Erni Sulistiawati Primate Research Center, Bogor Agricultural University, Bogor, Indonesia
  • Irma Herawati Suparto Primate Research Center, Bogor Agricultural University, Bogor, Indonesia
  • Retno Dumilah Esti Wijayanti Agency for the Assessment and Application of Technology, LAPTIAB-BPPT, Serpong, Indonesia
  • Tatas Hardo Panintingjati Brotosudarmo Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia


Objective: The objective of this research was to investigate the antihypercholesterolemic activity of herbal medicine which consisted of pigment-rich simplicia from higher and lower plants, as well as to determine its pigments composition.

Methods: Sprague-Dawley male rats were randomly divided into five cholesterol-rich diet groups as well as one negative control group with a normal diet, in order to investigate its effect on plasma lipid. The new herbal formulation was administered orally to induced-rats at three dosage levels, whereas simvastatin was used as a comparable generic drug. Pigments composition was determined by means of reversed-phase high-performance liquid chromatography (HPLC) using gradient protocol, monitored with diode array detector at 430 nm.

Results: The administration of herbal medicine was able to significantly reduce total plasma cholesterol and low-density lipoprotein-cholesterol (p<0.05) at a dose of 312.5 mg/kg of body weight/day compared to positive control group, and hence the atherogenic index was also lowered. The chromatographic analysis of pigments determination and quantification are reported correspondingly to support the possible active compounds data of hypocholesterolemic property.

Conclusion: The results obtained in this research work indicated the potency of pigment-rich lower plants to substitute the obtrusive exploration and dominant use of higher plants for treating dyslipidemia. 

Keywords: Herbal medicine, Lower plants, pigments, Cholesterol, Rats


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How to Cite
Limantara, L., R. Indrawati, D. E. Wijaya, E. Sulistiawati, I. H. Suparto, R. D. E. Wijayanti, and T. H. P. Brotosudarmo. “HYPOCHOLESTEROLEMIC EFFECT AND PIGMENTS COMPOSITION OF HERBAL MEDICINE CONTAINING HIGHER AND LOWER PLANTS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 9, no. 10, Nov. 2017, pp. 97-103, doi:10.22159/ijpps.2017v9i11.21133.
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