PHYTOCHEMICAL INVESTIGATION AND IN VITRO ANTIMICROBIAL SCREENING OF SANTALUM ALBUM SEEDS EXTRACTS


Gautam P. Vadnere, Md. Rageeb Usman, Santram Lodhi, Vaishali Patil

Abstract


Objective: Present study aimed phytochemical evaluation and antimicrobial screening of petroleum ether and ethanol extracts of Santalum album seeds.

Methods: Petroleum ether and ethanol extracts were screened for presence of chemical constituents. Petroleum ether extract was investigated detail by using chromatographic and spectroscopic methods. In vitro antimicrobial activity of both extracts were investigated using disc diffusion method on two gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus, gram negative Pseudomonas aeruginosa, Escherichia coli and fungus Candida albicans.

Results: Santalbic acid was identified in petroleum ether extract and content determined by HPTLC was 4.7%w/w. It was seen that petroleum ether extract have MIC value for B. subtilis, P. aeruginosa, E. coli and C. albicans were 78.125 µg/ml, 19.331 µg/ml, 625 µg/ml & 39.062 µg/ml respectively while MBC was 39.062 µg/ml, 4.882 µg/ml, 312.5 µg/ml & 9.765 µg/ml,  respectively. Petroleum ether extract showed MIC and MBC values for S. aureus was similar as 156.25µg/ml. So, the petroleum ether extract showed significant antimicrobial activity against both gram positive, gram negative and fungal strain.

Conclusions: The results of present investigations were indicative of possible high potency of petroleum ether extract due to santalbic acid which could serve as chemotherapeutic agent.


Keywords


Santalum album, antimicrobial; Santalbic acid; HPTLC; sandalwood

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About this article

Title

PHYTOCHEMICAL INVESTIGATION AND IN VITRO ANTIMICROBIAL SCREENING OF SANTALUM ALBUM SEEDS EXTRACTS

Keywords

Santalum album, antimicrobial; Santalbic acid; HPTLC; sandalwood

DOI

10.22159/ijpps.2017v9i11.21216

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 11, 2017 Page: 117-124

Online ISSN

0975-1491

Statistics

55 Views | 14 Downloads

Authors & Affiliations

Gautam P. Vadnere
Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Jalgaon 425107, M. S. India
India

Md. Rageeb Usman
Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Jalgaon 425107, M. S. India
India

Santram Lodhi
Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Jalgaon 425107, M. S. India
India

Vaishali Patil
Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Jalgaon 425107, M. S. India
India


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