PSIDIUM GUAJAVA L. EXTRACT INCREASES PLATELET COUNT THROUGH ENHANCEMENT OF STEM CELL FACTOR EXPRESSION IN THROMBOCYTOPENIC MICE MODEL

Authors

  • Nur Atik Departement of Anatomy, Physiology and Cellular Biology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia,
  • Susan Tarawifa Biomedical Science Master Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia,
  • Erda Avriyanti Department of Dermatology and Venereology, Faculty of Medicine, Universitas Padjadjaran /Dr.HasanSadikin General Hospital, Bandung, Indonesia,
  • Andri Reza Rahmadi Department of Internal Medicine, Faculty of Medicine, Universitas Padjadjaran/Dr.HasanSadikin General Hospital, Bandung, Indonesia,
  • Dany Hilmanto Department of Pediatrics, Faculty of Medicine, Universitas Padjadjaran /Dr.HasanSadikin General Hospital, Bandung, Indonesia

DOI:

https://doi.org/10.22159/ijpps.2018v10i1.21317

Keywords:

Guava extract, Platelet, Stem cell factor, Thrombopoietin

Abstract

Objective: The purpose of this study was to determine the mechanism through which guava extract increases platelet count.

Methods: Twenty male Swiss webster mice were divided into 4 group. Group I received 2.8 mg/20 g body weight of quinine and 1 ml of distilled water. Group II received 2.8 mg/20 grBW of quinine and 0.785 mg/20 grBW of guava extract. Group III received 1 ml distilled water and 0.785 mg/20 grBW of guava extract and Group IV only received 1 ml distilled water. After 14 days** treatment, platelet count was measured using Raas-Ecker method. SCF and TPO mRNA expressions in mice liver were assessed using qRT-PCR.

Results: The data showed that quinine administration reduced platelet count significantly (P<0.05). Psidium guajava L. extraction significantly enhanced platelet count in mice that were treated with quinine compared with quinine group (p<0.05). The mechanism was analysed further and our RT PCR data showed that stem cell factor mRNA expression significant increased (p<0.05) in quinine plus guava extract group compared to quinine group.

Conclusion: It indicated guava extract role in increasing megakaryopoiesis which resulted in an increase of platelet count. 

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Author Biographies

Nur Atik, Departement of Anatomy, Physiology and Cellular Biology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia,

Departement of Anatomy, Physiology and Cell Biology,
Assistant Professor.

Erda Avriyanti, Department of Dermatology and Venereology, Faculty of Medicine, Universitas Padjadjaran /Dr.HasanSadikin General Hospital, Bandung, Indonesia,

Department of Dermatology and Venereology, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia

Lecturer

Andri Reza Rahmadi, Department of Internal Medicine, Faculty of Medicine, Universitas Padjadjaran/Dr.HasanSadikin General Hospital, Bandung, Indonesia,

Department of Internal Medicine, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia

Assistant Professor

Dany Hilmanto, Department of Pediatrics, Faculty of Medicine, Universitas Padjadjaran /Dr.HasanSadikin General Hospital, Bandung, Indonesia

Department of Pediatric, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia

Professor

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Published

01-01-2018

How to Cite

Atik, N., S. Tarawifa, E. Avriyanti, A. R. Rahmadi, and D. Hilmanto. “PSIDIUM GUAJAVA L. EXTRACT INCREASES PLATELET COUNT THROUGH ENHANCEMENT OF STEM CELL FACTOR EXPRESSION IN THROMBOCYTOPENIC MICE MODEL”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 10, no. 1, Jan. 2018, pp. 23-26, doi:10.22159/ijpps.2018v10i1.21317.

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Original Article(s)