PSIDIUM GUAJAVA L. EXTRACT INCREASES PLATELET COUNT THROUGH ENHANCEMENT OF STEM CELL FACTOR EXPRESSION IN THROMBOCYTOPENIC MICE MODEL


Nur Atik, Susan Tarawifa, Erda Avriyanti, Andri Reza Rahmadi, Dany Hilmanto

Abstract


Objective: The purpose of this study was to determine the mechanism through which guava extract increases platelet count.

Methods: Twenty male Swiss webster mice were divided into 4 group. Group I received 2.8 mg/20 g body weight of quinine and 1 ml of distilled water. Group II received 2.8 mg/20 grBW of quinine and 0.785 mg/20 grBW of guava extract. Group III received 1 ml distilled water and 0.785 mg/20 grBW of guava extract and Group IV only received 1 ml distilled water. After 14 days** treatment, platelet count was measured using Raas-Ecker method. SCF and TPO mRNA expressions in mice liver were assessed using qRT-PCR.

Results: The data showed that quinine administration reduced platelet count significantly (P<0.05). Psidium guajava L. extraction significantly enhanced platelet count in mice that were treated with quinine compared with quinine group (p<0.05). The mechanism was analysed further and our RT PCR data showed that stem cell factor mRNA expression significant increased (p<0.05) in quinine plus guava extract group compared to quinine group.

Conclusion: It indicated guava extract role in increasing megakaryopoiesis which resulted in an increase of platelet count. 


Keywords


Guava extract, Platelet, Stem cell factor, Thrombopoietin

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About this article

Title

PSIDIUM GUAJAVA L. EXTRACT INCREASES PLATELET COUNT THROUGH ENHANCEMENT OF STEM CELL FACTOR EXPRESSION IN THROMBOCYTOPENIC MICE MODEL

Keywords

Guava extract, Platelet, Stem cell factor, Thrombopoietin

DOI

10.22159/ijpps.2018v10i1.21317

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 1, 2018 Page: 23-26

Online ISSN

0975-1491

Statistics

158 Views | 66 Downloads

Authors & Affiliations

Nur Atik
Departement of Anatomy, Physiology and Cellular Biology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia,
Indonesia

Susan Tarawifa
Biomedical Science Master Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia,
Indonesia

Erda Avriyanti
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Padjadjaran /Dr.HasanSadikin General Hospital, Bandung, Indonesia,
Indonesia

Andri Reza Rahmadi
Department of Internal Medicine, Faculty of Medicine, Universitas Padjadjaran/Dr.HasanSadikin General Hospital, Bandung, Indonesia,
Indonesia

Dany Hilmanto
Department of Pediatrics, Faculty of Medicine, Universitas Padjadjaran /Dr.HasanSadikin General Hospital, Bandung, Indonesia
Indonesia


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