GENOME-WIDE PREDICTION OF HUMAN PAPILLOMA VIRUS SPECIFIC T-CELL EPITOPES USING A COMBINATION OF MATRIX BASED COMPUTATIONAL TOOLS


Manikandan Mohan, Krishnan Sundar

Abstract


Objective: To predict the immunogenic epitopes from human papillomavirus (HPV) virus using matrix based computational tools.

Methods: In the present study, three matrix based algorithms, SYFPETHI, BIMAS and RANKPEP were used to predict the cytotoxic T lymphocyte (CTL) epitopes of HPV 16 and 18. The ability of the peptides to bind HLA A_0201, a most common allele, was evaluated using these algorithms. High scoring peptides were considered as potential binders.

Results: Evaluation of HPV 16 proteome resulted in the prediction of 249 peptides as potential binders. Out of these only 25 peptides were predicted as binders by all three algorithms. Analysis of HPV 18 predicted 215 peptides, as potential binders. Among the 215 peptides only 20 peptides were predicted as binders by all three algorithms.

Conclusion: The efficacy of these peptides in inducing a stronger immune response needs to be tested using in vitro and in vivo assays. The identified epitopes could be used in designing a novel epitope vaccine for HPV.


Keywords


Epitope prediction, CTL epitopes, Human papilloma virus, BIMAS, SYFPEITHI, RANKPEP

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About this article

Title

GENOME-WIDE PREDICTION OF HUMAN PAPILLOMA VIRUS SPECIFIC T-CELL EPITOPES USING A COMBINATION OF MATRIX BASED COMPUTATIONAL TOOLS

Keywords

Epitope prediction, CTL epitopes, Human papilloma virus, BIMAS, SYFPEITHI, RANKPEP

DOI

10.22159/ijpps.2017v9i11.21523

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 11, 2017 Page: 175-182

Online ISSN

0975-1491

Statistics

61 Views | 10 Downloads

Authors & Affiliations

Manikandan Mohan
Department of Biotechnology, Kalasalingam University, Krishnankoil - 626 126 Tamilnadu, India
India

Krishnan Sundar
Department of Biotechnology, Kalasalingam University, Krishnankoil - 626 126 Tamilnadu, India
India


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