DETERMINATION OF BIOACTIVE CONSTITUENTS OF LEAVES OF CORCHORUS AESTUANS (L.) BY GC - MS ANALYSIS
Objective: To investigate the bioactive constituents of ethanol extract of Corchorus aestuans (L.) using GC-MS technique.
Methods: The ethanol extract of leaves were subjected to GC- MS analysis using GC Clarus 500 (Perkin Elmer) with Elite- 5MS (5%Diphenyl / 95% Dimethyl poly siloxane, 30 mm x 0.25 mm x0.25Î¼m df). The 2 Î¼l extract sample injected and the components were separated using Helium (1 mL/min) as the carrier gas. Bioactive constituents were detected by the Turbo mass gold detector (Perkin Elmer) with the aid of the Turbomass 5.2 software.
Results: The analysis revealed the presence of fourteen different bioactive constituents namely 3, 7,11,15-tetramethyl-2-hexadecen-1-ol (5.6%), Trans-2-undecen-1-ol (1.26%), E-7-Tetradecenol (1.97%), n-Hexadecanoic acid (25.82%), Phytol (22.34%), 9,12,15-octadecatrienoic acid, methyl ester, (Z,Z,Z)- (20.23%), Docosanoic acid, ethyl ester (1.99%), 1-Eicosanol (2.11 %), 9,9-dimethoxybicyclo[3.3.1] nona-2,4-dione (0.60%), Heptadecanoic acid, heptadecyl ester (0.95%), Pentadecanoicacid,2,6,10,14-tetramethyl-,methylester(0.91%), 3-Hexadecycloxycarbonyl-5-(2-hydroxyethyl)-4-methylimidazolium ion (0.90%), Squalene (8.03%), Vitamin E (7.24%).
Conclusion: The presence of various bioactive compounds confirms the application of Corchorus aestuans in various disorders. However, isolation of individual phytochemical constituents may proceed to find a lead for herbal therapy.
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