NATURAL ANTIMICROBIAL ACTIVITY OF LAWSONIA INERMIS AND INDIGO TINCTORIA AGAINST CLINICALLY ISOLATED MICROORGANISMS
Objective: The present study aimed at evaluating the natural antimicrobial activity of Lawsonia inermis (L. inermis) and Indigofera tinctoria (I. tinctoria) against a reference and pathogenic clinically isolated strains compare with some antibiotics.
Methods: The antimicrobial activity of Lawsonia inermis (red henna) and Indigo tinctoria (black henna) was evaluated against clinically isolated strains from urinary tract and wounds infected patients. The six tested strains namely were Staphylococcus aureus, Enterococcus fecalis, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Candida albicans. The antimicrobial activity was evaluated by agar diffusion method and microplate dilution for measuring the minimum inhibitory concentration (MIC). An American Type Culture Collection (ATCC) of bacteria was used as a control for confirming the type of the isolated bacteria.
Results: Both red and black henna showed variable antimicrobial activity against tested bacteria and C. albicans. Alcoholic and oily extracts were more effective than water. Staphylococcus epidermis was significantly affected with water extract of black henna (20Â±1.1) and red henna (11Â±0.5). Nigella sativa oil extract with black henna had showed significant synergism effects against some microorganisms as Enterococcus fecalis (40Â±1.5), P. aeruginosa (15Â±0.9) and Candida albicans (18Â±0.6).
Conclusion: Black and red henna had antibacterial and antifungal activities. They exhibited synergistic effects when mixed with black seed oil.
2. Sukanya S, Sudisha J, Hariprasad P, Niranjana S, Prakash H, Fathima S. Antimicrobial activity of leaf extracts of Indian medicinal plants against clinical and phytopathogenic bacteria. Afr J Biotechnol 2009;8:6677â€“82.
3. Habbal O, Al-Jabri A, El-Hag A, Al-Mahrooqi Z, Al-Hashmi N. In vitro antimicrobial activity of Lawsoni Inermis linn (henna). A pilot study on the Omani henna. Saudi Med J 2005;26:69-72.
4. Hemem S. Activity of some plant extracts against common pathogens in bacterial skin infection: thesis MSc, College of Education, Basra University, Iraq; 2002.
5. Singh M, Jindal S, Kavia Z, Jangid B, Khem C. Traditional methods of cultivation and processing of henna. Henna, cultivation, improvement and trade. Jodhpur, India. Central Arid Zone Research Institute; 2005. p. 21-14.
6. Surveswaran S, Cai Y, Corke H, Sun M. Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. J Food Chem 2007;102:938-53.
7. Habbal O, Hasson S, El-Hag A, Al-Mahrooqi Z, Al-Hashmi N, Al-Bimani M, et al. Antibacterial activity of Lawsonia inermis Linn (Henna) against Pseudomonas aeruginosa. Asian Pac J Trop Biomed 2011;1:173-6.
8. Gull I, Sohail M, Aslam M, Athar M. Phytochemical, toxicological and antimicrobial evaluation of lawsonia inermis extracts against clinical isolates of pathogenic bacteria. Ann Clin Microbiol Antimicrob 2013;12:36.
9. Mastanaiah J, Prabhavathi N, Varaprasad B. In vitro anti-bacterial activity of leaf extracts of Lawsonia inermis. Int J PharmTech Res 2011;3:1045-9.
10. Saadabi M. Evaluation of Lawsonia inermis Linn. (Sudanese henna) leaf extracts as an antimicrobial agent. Res J Biol Sci 2007;2:419-23.
11. Jain N, Sharma M. Screening of Lawsonia inermis essential oil against fungi causing dermatophytic infection in human. Asian J Pharm Clin Res 2016;4:67-9.
12. Chanayath N, Lhieochaiphant S, Phutrakul S. Pigment extraction techniques from the leaves of Indigofera tinctoria Linn. and Baphicacanthus cusia Brem. and chemical structure analysis of their major components. CMU J 2002;1:2.
13. Ensley B, Ratzkin B, Osslund T, Simon M, Wackett L, Gibson D. Expression of naphthalene oxidation genes in Escherichia coli results in the biosynthesis of indigo. Science 1983;222:167-9.
14. Heo B, Park Y, Kim K, Cho J, Boo H. Antioxidant enzyme activity and antimicrobial activity of Isatis tinctoria extract. Korean J Plant Res 2012;25:543â€“9.
15. Vijayan M, Jacob K, Govindaraj Y. Antibacterial activity and mutagenicity of leaves of Indigofera tinctoria Linn. J Exp Integrative Med 2012;2:263-9.
16. Saadabi M. Evaluation of Lawsonia inermis Linn. (Sudanese Henna) leaf extracts as an antimicrobial agent. Res J Biol Sci 2007;2:419â€“23.
17. Renukadevi K, Suhani S. Determination of antibacterial, antioxidant and cytotoxicity effect of Indigofera tinctoria. Int J Pharmacol 2011;7:356-62.
18. Singh A, Singh D. Molluscicidal activity of Lawsonia inermis and its binary and tertiary combinations with other plant derived molluscicides. Indian J Exp Biol 2001;39:263-8.
19. Ushimaru P, Mariama T, Luiz C, Di Luciano B, Ary F. Antibacterial activity of medicinal plant extract. Braz J Microbial 2007;38:717â€“9.
20. Annavarapu T, Renuka P, Akhil P, Divya P, Devi Priyanka P. Evaluation of the anti-inflammatory activity of the combination of ethanol extracts Azadirachta Indica (neem) and Lawsonia inermis (henna). Asian J Pharm Clin Res 2016;9:256-8.
21. Zohary D, Hopf M. Domestication of plants in the old world. 3rd edition Oxford University; 2000. p. 206.
22. Jain K, Choudhury P, Sharma M. Total flavonoid quantification and to study antibacterial potency of extracts of Butea Monosperma flowers, Nigella Sativa seeds and vitex Agnus Castus laves. Int J Curr Pharm Res 2007;9:71-4.
23. Hassieb A. Melanin is the secret of Nigella Sativa. Al-Faisal Sci J 2006;4:110â€“43.
24. Gazem R, Chandrashekariah S. Pharmacological properties of Salvia hispanica (CHIA) seeds: a review. J Crit Rev 2016;3:63-77.
25. Rios J, Recio M, Villar A. Screening methods for natural products with antibacterial activity. Rev Literature J Ethnopharmacol 1998;23:127-49.
26. Eloff J. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med 1998;64:711-3.
27. Bauer A, Kirby W, Sherris J, Turok M. Antibiotic susceptibility testing by standard single disk method. Am J Clin Pathol 1966;45:493-6.
28. NCCL. National committee for clinical laboratory standards for antimicrobial disk susceptibility testing. 12th information supplements (M100-S12). Wayne PA. NCCL; 2002.
29. Ali K, Al-hood F, Obad K, Alshakka M. Phytochemical screening and antibacterial activity of Yemeni henna (Lawsonia Inermis) L. leaves extracts on Staphylococcus aurues isolates. Bas J Vet Res 2013;12:256-65.
30. Edany A, Y Khudair B, Nawras N. Antibacterial activity of Lawsonia inermis leaves extracts on Staphylococcus aureus isolates. Bas J Vet Res 2013;12:256-65.
31. Papageorgiou V, Assimopoulou A, Couladouros E, Hepworth D, Nicolaou KC. The chemistry and biology of alkannin, shikonin, and related naphthazarin natural products. Angew Chem 1999;38:270â€“300.
32. Hussain T, Arshad M, Khan S, Sattar H, Qureshi M. In vitro screening of methanol plant extracts for their antibacterial activity. Pak J Bot 2011;43:531â€“8.
33. Karou D. Antibacterial activity of alkaloids from Sida acuta. Afr J Biotechnol 2005;4:1452-7.
34. Nikaido H, Vaara M. Molecular basis of bacterial outer membrane permeability. Microbiol Rev 1985;1:1-32.
35. Senthilkumar A. Venkatesalu V. Photochemical analysis and antibacterial activity of the essential oil of Clausena anisata (Willd) hook. F. ex benth. Int J Integrative Biol 2009;5:116-20.
36. Bakathir H, Abbas N. Antibacterial activity of alkaloids from Sida acuta. Afr J Biotechnol 2005;4:1452-7.
37. Ali K, Al-hood F, Obad K, Alshakka M. Phytochemical screening and antibacterial activity of Yemeni henna (Lawsonia Inermis) against some bacterial pathogens. IOSR-JPBS 2016;2:24-7.
38. Arun P, Purushotham K, Jayarani J, Vasantha K. In vitro antibacterial activity and flavonoid contents of Lawsonia inermis (Henna). Int J PharmTech Res 2010;2:1178-81.