HEPATO-NEPHROPROTECTIVE ACTIVITY OF NIGELLA SATIVA OIL ON PARACETAMOL-INDUCED NEW ZEALAND RABBITS (ORYCTOLAGUS CUNICULUS)

  • Nadhira M. Dinar Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km.21 Jatinangor, West Java, Indonesia 45363
  • Sheila Pratiwi Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km.21 Jatinangor, West Java, Indonesia 45363
  • Rain Kihara Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km.21 Jatinangor, West Java, Indonesia 45363
  • Nadia G. Paramita Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km.21 Jatinangor, West Java, Indonesia 45363
  • Nazilla R. Fathurrahman Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km.21 Jatinangor, West Java, Indonesia 45363
  • Jutti Levita Scientific Consortium for Drug Discovery and Development, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km.21 Jatinangor, West Java, Indonesia 45363 http://orcid.org/0000-0002-4578-4174

Abstract

Objective: The objective of this work was to investigate the hepato-nephroprotective activity of Nigella sativa (Ranunculaceae) oil on paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus).

Methods: Hepato-nephroprotective activity of Nigella sativa oil was demonstrated on six groups of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus) aged 3-4 mo, three in each group (2 males, 1 female). Group I was normal control (water 1.0 ml/kg of body weight per oral), group II was negative control (water 1.0 ml/kg of body weight per oral), group III was positive control (silymarin 100 mg/kg of body weight per oral), group IV-VI were treated with Nigella sativa oil (NSO) dose of 0.5 mg/kg of body weight, 1.0 mg/kg of body weight, and 2.0 mg/kg of body weight per oral, respectively, for 15 d. At the 16th day, rabbits in group II-VI were induced with paracetamol at a dose of 600 mg/kg of body weight per oral. At the 23rd day the animals were measured for their clinical biochemistry parameters and histological examination.

Results: Paracetamol administration dose of 600 mg/kg of BW resulted in the elevation of serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), and ureum-N levels of the animals, particularly in group II which was treated only with paracetamol. Normal histology of the liver defines the clear shape of the terminal hepatic venule (THV)/central vein (CV) and sinusoids, whereas that of the kidney defines clear shape of the Bowman capsule and glomerulus shape. Qualitative histological examination of the liver showed that the THV/CV in all groups was normal, however in the paracetamol-treated group, the sinusoids were dilated, necrosis and mass apoptosis were detected. Dilated sinusoids were observed in the silymarin group and in the lower and medium doses of NSO groups. In the highest dose of NSO group the THV/CV and sinusoids were normal, but a local apoptosis and fat degeneration were detected. Qualitative histological examination of the kidney indicated that there was no abnormality of the glomerulus shape, however, mass apoptosis and local necrosis of the kidney were found in the paracetamol-treated group and the silymarin-treated group. The lowest dose of the NSO-treated group showed a normal shape of glomerulus and Bowman capsule, normal apoptosis. No necrosis was observed in the rabbit’s kidney. Higher doses of NSO groups indicated a normal glomerulus shape and Bowman capsule, mass apoptosis and local necrosis.

Conclusion: In this study, Nigella sativa oil could maintain the normality of the THV/CV and sinusoids in the liver of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus). Normal glomerulus shape and Bowman capsule were also confirmed in the kidney of paracetamol-induced animals.

Keywords: Acetaminophen, Black cumin, Black seeds, Cytochrome P450, Fennel flower, Ranunculaceae

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References

1. Mishra S, Aeri V, Katare DP. Hepatoprotective medication for a liver injury. World J Pharm Pharm Sci 2014;3:891-932.
2. Ortega-Alonso A, Stephens C, Lucena MI, Andrade RJ. Case characterization, clinical features and risk factors in drug-induced liver injury. Int J Mol Sci 2016;17:714-36.
3. Andrade RJ, Robles M, Fernández-Castaner A, Lopez Ortega S, Lopez-Vega MC, Lucena MI. Assessment of drug-induced hepatotoxicity in clinical practice: a challenge for a gastroenterologist. World J Gastroenterol 2007;13:329-40.
4. Pradhan SC, Girish C. Hepatoprotective herbal drug, silymarin from experimental pharmacology to clinical medicine. Indian J Med Res 2006;124:491-504.
5. McBride A, Augustin KM, Nobbe J, Westervelt P. Silybum marianum (milk thistle) in the management and prevention of hepatotoxicity in a patient undergoing reinduction therapy for acute myelogenous leukaemia. J Oncol Pharm Pract 2012;18:360-5.
6. Saller R, Meier R, Brignoli R. The use of silymarin in the treatment of liver diseases. Drugs 2001;61:2035-63.
7. Rainone F. Milk thistle. Am Fam Physician 2005;72:1285-8.
8. Roy A, Bhoumik D, Sahu RK, Dwivedi J. Medicinal plants used in liver protection-A review. UK J Pharm Biosci 2014;2:23-33.
9. Venkatachallam SKT, Pattekhan H, Divakar S, Kadimi US. The chemical composition of Nigella sativa L. seed extracts obtained by supercritical carbon dioxide. J Food Sci Technol 2010;47:598–605.
10. Krishna M. Microscopic anatomy of the liver. CLD 2013;2 Suppl 1:S4-7.
11. Al-Amoudi WM. Protective effects of fennel oil extract against sodium valproate-induced hepatorenal damage in albino rats. Saudi J Biol Sci 2017;94:915-24.
12. Payasi A, Ankush G, Manu C, Vivek DK, Mohan SB, Sanjay SM. Sub-acute toxicity studies of paracetamol infusion in Mus musculus mice. Int J Drug Dev Res 2010;2:157-63.
13. Himaja N, Shama N. Herbal wealth for hepatotoxicity. Asian J Pharm Clin Res 2015;8:3-9
14. Nitin M, Ifthekar SQ, Mumtaz M. Evaluation of hepatoprotective and nephroprotective activity of aqueous extract of Vigna mungo (Linn.) Hepper on rifampicin-induced toxicity in albino rats. Int J Health Allied Sci 2012;1:85-91.
15. Daba MH, Abdel-Rehman MS. Hepatoprotective activity of thymoquinone in isolated rat hepatocytes. Toxicol Lett 1998;95:23-9.
16. Nithya G, Ilakkia A, Sakthisekaran D. In silico docking studies on the anti-cancer effect of thymoquinone on interaction with phosphatase and tensin homolog located on chromosome 10q23: a regulator of PI3K/AKT pathway. Asian J Pharm Clin Res 2015;8:192-5.
17. Yesmin F, Rahman Z, Dewan JF, Helali AM, Rahman NIA, Alattraqchi AG, et al. Hepatoprotective role of the aqueous and n-hexane extracts of Nigella sativa Linn. in experimental liver damage in rats. Asian J Pharm Clin Res 2013;6 Suppl 3:205-9.
18. Shaalan S, El-Wakkad ASE, Saleh H, Deab A. Protective effect of l-carnitine and baker yeast Saccharomyces cerevisiae against hepatic toxicity induced by valproate as an antiepileptic drug in rats. Int J Pharm Pharm Sci 2015;7:89-95.
19. Ramadan A, Soliman G, Mahmoud SS, Nofal SM, Abdelrahman RF. Hepatoprotective and hypnotherapeutic effects of propolis against D-galactosamine/lipopolysaccharide-induced liver damage in rats. Int J Pharm Pharm Sci 2015;7:372-8.
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How to Cite
Dinar, N. M., S. Pratiwi, R. Kihara, N. G. Paramita, N. R. Fathurrahman, and J. Levita. “HEPATO-NEPHROPROTECTIVE ACTIVITY OF NIGELLA SATIVA OIL ON PARACETAMOL-INDUCED NEW ZEALAND RABBITS (ORYCTOLAGUS CUNICULUS)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 9, no. 10, Nov. 2017, pp. 225-8, doi:10.22159/ijpps.2017v9i11.21854.
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