THE N-HEXANE FRACTION OF MYRMECODIA PENDANS INHIBITS CELL SURVIVAL AND PROLIFERATION IN COLON CANCER CELL LINE


Muhammad Hasan Bashari, Saras Hidayat, Yasmin Anissa Robles Ruswandi, Tenny Putri, Nurul Qomarilla, Resti Gradia Dwiwina, Dikdik Kurnia, Mieke H. Satari, Fathul Huda

Abstract


Objective: Despite advanced treatment options available for colorectal cancer, many reported resistance and unresponsiveness to conventional chemotherapeutic agents. Therefore, it is urgent to discover a novel drug for colon cancer. Sarang Semut (Myrmecodia pendans), an Indonesian native plant, has been studied extensively due to its anti-cancer profiles. This study aimed to evaluate the anti-tumour activity of Sarang Semut in colon cancer cells.

Methods: We evaluated cytotoxic activity of methanol extract as well as n-hexane and ethyl acetate fraction towards colon cancer cell lines (Caco-2 and HCT-116 cells) utilizing 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. The most potent fraction was evaluated further in inhibiting cell survival using MTT assay and cell proliferation using trypan blue exclusion assay as well as a clonogenic assay.

Results: Our data showed that the n-hexane fraction of Sarang Semut induces more cell death than the methanol extract and ethyl acetate fraction. Therefore, we analyzed the n-hexane fraction further and found that the inhibitory concentration 50% (IC50) of the n-hexane fraction was 24 and 30 parts per million (ppm) for Caco-2 and HCT-116 cells, respectively. Moreover, it inhibited cell growth as well as cell colony formation, in particular, shown by the plating efficiency (P<0.05) and colony area per seed (P<0.01) of the control group were different to the treatment group.

Conclusion: The n-hexane fraction of Sarang Semut demonstrates a high potential antitumor activity in colon cancer cell line.


Keywords


Colorectal cancer, Myrmecodia pendans, Sarang Semut, cytotoxic assay, clonogenic assay

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About this article

Title

THE N-HEXANE FRACTION OF MYRMECODIA PENDANS INHIBITS CELL SURVIVAL AND PROLIFERATION IN COLON CANCER CELL LINE

Keywords

Colorectal cancer, Myrmecodia pendans, Sarang Semut, cytotoxic assay, clonogenic assay

DOI

10.22159/ijpps.2018v10i1.21882

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 1, 2018 Page: 108-112

Online ISSN

0975-1491

Authors & Affiliations

Muhammad Hasan Bashari
Departement of Pharmacology and Therapy, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia,
Indonesia

Saras Hidayat
Undergraduate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
Indonesia

Yasmin Anissa Robles Ruswandi
Undergraduate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
Indonesia

Tenny Putri
Laboratory of Advanced Biomedicine, Universitas Padjadjaran, Bandung, Indonesia,
Indonesia

Nurul Qomarilla
Laboratory of Advanced Biomedicine, Universitas Padjadjaran, Bandung, Indonesia,
Indonesia

Resti Gradia Dwiwina
Departement of Anatomy, Physiology, and Cellular Biology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia,
Indonesia

Dikdik Kurnia
Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia,
Indonesia

Mieke H. Satari
Departement of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
Indonesia

Fathul Huda
Biomedical Science Master Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia, Departement of Anatomy, Physiology, and Cellular Biology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia,
Indonesia


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