ANTIOXIDANT AND HEPATO PROTECTIVE ACTIVITY OF VARIOUS EXTRACTS OF INDIGOFERA BARBERI GAMBLE AGAINST D-GALACTOSAMINE INDUCED TOXICITY IN RATS

  • N Srinivasan Department of Pharmacy, FEAT, Annamalai University, Annamalai Nagar 608002 Tamil Nadu, India
  • Sathyanarayana. D Department of Pharmacy, FEAT, Annamalai University, Annamalai Nagar 608002 Tamil Nadu, India

Abstract

Objective: To investigate Antioxidant and Hepatoprotective activity of various extracts of Indigofera barberi Gamble against D-Galactosamine induced toxicity in rats.

Methods: Indigofera barberi Gamble is used traditionally as folk system of medicine in India for the treatment of liver diseases, and as a liver tonic. Petroleum ether (PEEIB), chloroform(CEIB) and ethanolic extracts (EEIB) of Indigofera barberi Gamble (aerial part of the plants extracts, 200mg/kg, p. o) was tested for its hepatoprotective effect against D-galactosamine induced hepatic damage in rats at a dose of (400mg/kg, i. p) to induce hepatotoxicity and Silymarin (25 mg/kg, i. p.) was administered as standard drug.

Results: The biochemical parameters of liver tissue LPO, GSH, GPX, GST, SOD and CAT values were significantly (p<0.01) altered against ethanolic extracts treatment of Indigofera barberi Gamble and were comparable with standard drug silymarin.

Conclusion: Animal pretreatment with petroleum ether and chloroform extracts of Indigofera barberi Gamble did not change above mentioned parameters significantly. The histopathological studies also substantiates the results and hence, it can be concluded from the present study that, the ethanolic extracts of Indigofera barberi Gamble has appreciable hepatoprotective potential in albino rats against D-galactosamine induced hepatic damage.

 

Keywords: D-Galactosamine, Silymarin, Indigofera barberi, Biochemical parameters, Ethanolic extract.

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References

1. Sambrekar SN, Patil PA, Kangralkar VA. Protective activity of Mussaenda frondosa leaf extracts against paracetamol induced hepatic damage in wistar rats. J Pharm Res 2010;3(4):711-3.
2. Ravikumar V, Shivashangari KS, Devaki T. Hepatoprotective activity of Tridax procumbens against D-Galactosamine/ lipopolysaccharide-induced hepatitis in rats. J Ethnopharmacol 2005;101(1-3): 55–60.
3. Wu YL, Lian LH, Jiang YZ, Nan JX. Hepatoprotective effects of Salidroside on fulminant hepatic failure induced by D-Galactosamine and lipopolysaccharide in mice. J Pharm Pharmaco 2009;61(10):1375–82.
4. Morrison DC, Danner RL, Dinarello CA, Munford RS, Natanson C, Pollack M, et al. Bacterial endotoxins and pathogenesis of gram-negative infections: current status and future direction. J Endotoxin Res 1994;1(2):71–83.
5. Madhavachetty K, Sivaji K, Tulsirao K. Flowering parts of Chittoor district, Andhra Pradesh, India. 1st Edition. Students offset printers;2008. p. 91-2.
6. Gamble JS. Flora of the Presidency of Madras, Vol. I, Published under the authority of the secretary of state for India in council;2001. p. 309-10.
7. Ohkawa H, Onishi N, Yagi K. Assay for lipid peroxides in animal tissue by Thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
8. Habig WH, Pabst MJ, Jackoby WB. Glutathione-S transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130-9.
9. Hochstein P, Utley H. Hydrogen peroxide detoxication by glutathione peroxidase and catalase in rat liver homogenates. Molecular Pharmaco 1968;16:574–9.
10. Moron MS, Dipieree JW, Mannerwik B. Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Biochimica Biophysica Acta 1979;582 (1):67–78.
11. Oynagui Y. Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal Biochem 1984;142(2):290–6.
12. Bergmeyer HU. Measurement of catalase activity. Biochemische Zeitschrift 1955;327:255–96.
13. Millard PR. Essential Histopathology, Blackwell Scientific Publications, London; 1990. p. 1-337.
14. Drotman RB, Lawhorn GT. Serum enzymes are indicators of chemical induced liver damage. Drug Chemical Toxicology 1978;1(2):163-71.
15. Frei B, Hidgon JV. Antioxidant activity of tea polyphenols in vivo: evidence from animal studies. J Nut 2003;133(10):3275-84.
16. El-Mofty SK, Scrutton MC, Serroni A, Nicolini C, Farber JL. Early, reversible plasma membraneinjury in galactosamine-induced liver cell death. Am J Pathology 1975;79(3):579–96.
17. The OECD Guideline for Testing of Chemical: 423-Acute Oral Toxicity – Acute Toxic Class Method. OECD, Paris; 2001. p. 1–14.
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Srinivasan, N., and S. D. “ANTIOXIDANT AND HEPATO PROTECTIVE ACTIVITY OF VARIOUS EXTRACTS OF INDIGOFERA BARBERI GAMBLE AGAINST D-GALACTOSAMINE INDUCED TOXICITY IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 10, 1, pp. 111-4, https://innovareacademics.in/journals/index.php/ijpps/article/view/2202.
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