OXIDATIVE DNA DAMAGE PROTECTIVE AND HEPATOPROTECTIVE ACTIVITY OF TERMI-NALIA BELLERICA (BAHEDA)

Rasna Gupta; Ram Lakhan Singh; Andab Pant

doi:10.22159/ijpps.2018v10i1.22455

Authors

Rasna Gupta Nutraceutical Laboratory, Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India
Ram Lakhan Singh Nutraceutical Laboratory, Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India
Andab Pant Division of In Vitro Toxicology, CSIR-Indian Institute of Toxicology Research, Lucknow-226001, U.P., India

DOI:

https://doi.org/10.22159/ijpps.2018v10i1.22455

Keywords:

DNA damage, CCl4, HepG2, Hepatoprotective, Terminalia bellerica

Abstract

Objective: To investigate the ethanolic extracts of Terminalia bellerica for its in vitro DNA damage protective activity against hydroxyl radical (OH^â€¢) and hepatoprotective activity against CCl₄induced toxicity in HepG2 liver cells.

Methods: The DNA damage assay was performed using supercoiled pBR322 plasmid DNA with Fenton's reagent. Protection of human liver-derived HepG2 cells against CCl₄ induced damage was determined by trypan blue exclusion assay and Tetrazolium bromide salt MTT assay. Liver cells were pre-exposed to 0.5 Âµmol/ml of CCl₄ for 60 min at room temperature and screening assay was carried out in HepG2 cells to evaluate the cytotoxicity of tested extracts with concentration 0.001 to 100 Âµg/ml.

Results: The low dose (0.001-0.1 Âµg/ml) of leaf and bark has an effect on the percentage viability (101Â±4.04) and 97Â±4.04) for leaf and bark extracts respectively) of HepG2 cells. Whereas, the percentage cell viability 99Â±2.89 and 103Â±4.04 were found for fruit pulp and seed extracts respectively. The uniform DNA damage protective activity was observed in a dose-dependent manner for leaf, fruit pulp, seed and bark extracts of T. bellerica.

Conclusion: Leaf and bark of T. bellerica exhibit 10 fold less toxicity compared to fruit pulp and seed during CCl₄exposure on HepG2 cells suggesting that leaf and bark has more therapeutic potential against hepatotoxicity.

Downloads

Download data is not yet available.

Author Biography

Rasna Gupta, Nutraceutical Laboratory, Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India

Is a State Governmnt University, Department of Biochemistry

References

Subramaniam S, Khan HBH, Elumalai N, Lakshmi SYS. Hepato-protective effect of ethanolic extract of the whole plant of An-drographis paniculata against CCl4 induced hepatotoxicity in rats. Comp Clin Path 2015;24:1-7.

Gu X, Manautou JF. Molecular mechanisms underlying chemi-cal liver injury. Expert Rev Mol Med 2012;14:e4.

CichoÅ¼ Lach H, Michalak A. Oxidative stress as a crucial factor in liver diseases. World J Gastroenterol 2014;20:8082-91.

Lahlou M. The success of natural products in drug discovery. Pharmacol Pharm 2013;4:17-31.

Prajapati ND, Purohit SS, Sharma AK, Kumar T. A handbook of medicinal plants. 1st ed. India: Agrobios Publishers; 2003.

Singh R, Singh SK, Arora S. Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn Food Chem Toxicol 2007;45:1216-23.

Gupta R, Singh RL, Dwivedi N. In vitro antioxidant activity and GC-MS analysis of the ethanolic extracts of Terminalia belleri-ca ROXB (Baheda). Int J Pharm Pharm Sci 2016;8:275-82.

Lee JC, Kim HR, Kim J, Yang YS. Antioxidant property of an eth-anol extract of Optunia ficus-indica Saboten. J Agric Food Chem 2002;50:6490-6.

Kashyap MP, Singh AK, Siddiqui MA. Caspase cascade regulated mitochondria-mediated apoptosis in monocrotophos exposed PC12 cells. Chem Res Toxicol 2010;23:1663-72.

Pant AB, Agarwal AK, Sharma VP, Seth PK. In vitro cytotoxicity evaluation of plastic biomedical devices. Hum Exp Toxicol 2001;20:412-7.

Singh P, Singh RL, Kakkar P. Antioxidant, DNA damage protec-tive and hepatoprotective activities of Amorphophallus cam-panulatus. Int J Pharm Pharm Sci 2016;8:330-8.

Hiraganahalli BD, Chinampudur VC, Dethe S, Mundkinajeddu D, Pandre MK, Balachandran J, et al. Hepatoprotective and antiox-idant activity of standardized herbal extracts. Pharmacogn Mag 2012;8:116-23.

Deena Priscilla H, Jasmine R. Evaluation of in vitro anticancer activity of Terminalia chebula and identification of phytocompounds by GC MS analysis. J Chem Pharm Res 2016;8: 683-8.

Vasanth PR, Chandrasekhar RH, Vijayan P, Dhanaraj SA, Rao MC, Rao VJ, et al. In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves. In-dian J Pharmacol 2010;42:99-104.

Knockaert L, Berson A, Ribault C. Carbon tetrachloride-mediated lipid peroxidation induces early mitochondrial al-terations in mouse liver. Lab Invest 2012;92:396-410.

Talwar S, Jagani HV, Nayak PG. Toxicological evaluation of Terminalia paniculata barks extract and its protective effect against CCl4-induced liver injury in rodents. BMC Complementary Altern Med 2013;13:127-33.

Haidry MT, Malik A. Hepatoprotective and antioxidative ef-fects of Terminalia Arjuna against cadmium provoked toxicity in albino rats (Ratus Norvigicus). Biochem Pharmacol 2014;3:130.

Saroya AS. Herbalism phytochemistry and Ethnopharmacology. CRC Press: Taylor and Francis Group; 2011. p. 357-61.

Devi PN, Kaleeswari S, Poonkotha M. Antimicrobial activity and phytochemical analysis of fruit extracts of Terminalia bel-lerica. Int J Pharm Pharm Sci 2014;6:639-42.