Sudhanshu Sekhar Rout, Sovan Pattanaik, Sudam Chandra Si, Prakash Mohanty


Objective: These studies focus on the interaction between two clinically active antiparkinsonian drugs L-dopa (L) and carbidopa (C) with the cis-[Cr(C2O4)2(H2O)2]-and evaluation of the synthesized product from a coordination chemistry aspect with respect to the possibility of its antioxidant activity and its therapeutic application in the treatment of Parkinson disease.

Methods: The resulting synthesized complexes were characterized by UV-VIS and FTIR spectroscopy. Evaluation of antioxidant activities of this cis-[Cr(C2O4)2(H2O)2]--L-dopa(ML), cis-[Cr(C2O4)2(H2O)2]--carbidopa(MC) and standard butylated hydroxytoluene (BHT) were carried out by using 1,1-diphenyl-1-picrylhydrazyl free radical (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cations and hydrogen peroxide method.

Results: The results of spectral analysis of the synthesized products indicate that complexes have a Cr(III) ion coordinated via the carboxylic and amino group. In the reduction of radical DPPH· and the formation of radical monocation ABTS·+the ability to scavenge radical was measured in these experiments by the discoloration of the solution. However, in hydrogen peroxide method, the increased in absorbance showing its scavenging potential. The scavenging capacity of the test compounds and standard on the DPPH, ABTS·+, H2O2 decreased in the order BHT>ML>MC>C>L which were 98.4, 96.4, 86.4, 68.3, 49.7% for DPPH, BHT>ML>L>MC>C which were 99.3, 96.9, 96.3,66.6, 53.4% for ABTS·+, BHT>ML>MC>L>C which were 68.8%, 52.4%, 49.6%, 43.1% and 37.7% for H2O2 at the concentration of 50 µg/ml, respectively.

Conclusion: The experimental findings showed that cis-[Cr(C2O4)2(H2O)2]--levodopa and cis-[Cr(C2O4)2(H2O)2]--carbidopa are having higher antioxidant potential than Levodopa and carbidopa although not superior to that of standard compound.


Levodopa, Carbidopa, Cis-diaqua-bis(oxalato)chromate(III), DPPH, ABTS

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Kiss T, Gergely A. Copper (II) and nickel (II) ternary complexes of L-Dopa and related compounds. J Inorg Biochem 1985;25:247-59.

Sanders LH, Greenamyre JT. Oxidative damage to macromolecules in human Parkinson disease and the rotenone model. Free Radical Biol Med 2013;62:111-20.

Halliwell B, Gutteridge JM. Free radicals in biology and medicine. Clarendon Press: Oxford. 2nd Ed.; 1989. p. 23-30.

Jodko-Piorecka K, Litwinienko G. Antioxidant activity of dopamine and L-DOPA in lipid micelles and their cooperation with an analogue of α-tocopherol. Free Radical Biol Med 2015;83:1-11.

Balik J, Kyselakova M, Vrchotova N, Triska J, Kumsta M, Veverka J, et al. Relations between polyphenols content and antioxidant activity in vine grapes and leaves. Czech J Food Sci 2008;26:25-32.

Pawar V, Joshi S, Uma V. Antibacterial and antioxidant properties of macrocyclic Schiff bases with vanadium (V) complexes. J Chem Pharm Res 2011;3:169-75.

Kostova I, Saso L. Advances in research of schiff-base metal complexes as potent antioxidants. Curr Med Chem 2013;20:4609-32.

Halliwell B. How to characterize a biological antioxidant. Free Radical Res Communications 1990;9:1-32.

Baral DK, Rout SS, Behera J, Si SC, Mohanty P. Kinetics and mechanism of interaction of hexaaquachromium (III) with l-Dopa in an aqueous medium: comparative antiparkinsonian studies. Transition Metal Chem 2011;36:231-6.

Aziza BK, Tofiqa DI. Kinetic and equilibrium comparison of the complexation reaction of Cis-and trans-forms of Cr(ox)2(H2O)2. xH2O with alanine. Int J Chem Environ Eng 2012;3:34-8.

Rout SS, Kar DM, Pattanaik S, Si SC, Mohanty P. Kinetics and mechanism of reaction of cis-[Cr(C2O4)2(H2O)2]-with L-Dopa in an aqueous medium: comparative antiparkinsonian studies. Asian J Chem 2017;29:1555-60.

Blois MS, Antioxidant determinations by the effect of a stable free radical. Nature 1958;26:1199-200.

Gülçin İ. Antioxidant and antiradical activities of L-Carnitine. Life Sci 2006;78:803-11.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolarization assay. Free Radical Biol Med 1999;26:1231-7.

Gülçin I. Antioxidant activity of caffeic acid (3,4-dihydroxy cinnamic acid). Toxicology 2006;217:213-20.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention and cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003-8.

Siddiqi ZA, Khalid M, Kumar S, Shahid M, Noor S. Antimicrobial and SOD activities of novel transition metal complexes of pyridine-2, 6-dicarboxylic acid containing 4-picoline as an auxiliary ligand. Eur J Med Chem 2010;45:264-9.

Delaney S, Pascaly M, Bhattacharya PK, Han K, Barton JK. Oxidative damage by ruthenium complexes containing the dipyridophenazine ligand or its derivatives: a focus on intercalation. Inorganic Chem 2002;41:1966-74.

Serbest K, Colak A, Guner S, Karabocek S, Kormali F. Copper (II)–manganese (II) complexes of 3, 3′-(1, 3-propanediyldiimine) bis-(3-methyl-2-butanone) dioxime with superoxide dismutase-like activity. Transition Metal Chem 2001;26:625-9.

Vogel AI. Text Book of Practical Organic Chemistry. 5Th Edition. Longman, London; 1989.

Patel KS, Patel JC, Dholariya HR, Patel VK, Patel KD. Synthesis of Cu (II), Ni (II), Co (II), and Mn (II) complexes with ciprofloxacin and their evaluation of the antimicrobial, antioxidant and anti-tubercular activity. Open J Metal 2012;2:49-59.

Koksal E, Gulcin İ, Beyza S, Sarikaya O, Bursal E. In vitro antioxidant activity of silymarin. J Enzyme Inhibition Med Chem 2009;24:395-405.

Brand-Williams W, Cuvelier ME, Berset CL. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 1995;28:25-30.

Pattanaik S, Si SC, Nayak SS. Evaluation of free radical scavenging activity, wound healing activity and estimation of phenolic, flavonoid and proanthocyanidine contents of the plant “Crateva magna”. Asian J Pharm Clin Res 2012;5(Suppl 3):168-71.

Shirwaikar A, Shirwaikar A, Rajendran K, Punitha ISJ. In vitro antioxidant studies on the benzyl tetra isoquinoline alkaloid berberin. Biol Pharm Bull 2006;29:1906-10.

Wolfeden BS, Willson RL. Radical cations as reference chromogen in kinetic studies of one-electron transfer reactions: pulse radiolysis studies of 2,29-azinobis-(3-ethylbenzthiazoline-6-sulfonate). Chem Soc Perk Trans 1982;2:805-12.

Miller DD. Mineral. In: Fennema OR. Ed. Food Chemistry. Marcel Dekker, New York; 1996. p. 618-49.

Gülçin I, Elias R, Gepdiremen A, Boyer L. Antioxidant activity of lignans from the fringe tree (Chionantus virginicus L.). Eur Food Res Technol 2006;223:759-67.

Gulcin I. Comparison of in vitro antioxidant and antiradical activities of L-tyrosine and L-Dopa. Amino Acids 2007;32:431-8.

Hyslop PA, Hinshaw WA, Schraufstatter IU, Sauerheber RD, Spragg RG, Jackson JH, et al. Mechanism of oxidation-mediated cell injury. The glycolytic and mitochondrial pathways of ADP phosphorylation are major intracellular targets inactivated by hydrogen peroxides. J Biol Chem 1988;263:1665-75

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Levodopa, Carbidopa, Cis-diaqua-bis(oxalato)chromate(III), DPPH, ABTS





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International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 1, 2018 Page: 137-141

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Sudhanshu Sekhar Rout
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Bhubaneswar, India

Sovan Pattanaik
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Bhubaneswar, India

Sudam Chandra Si
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Bhubaneswar, India

Prakash Mohanty
Department of Chemistry, Ravenshaw University, Cuttack, India

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