PHYTOCHEMICAL, ANTI-INFLAMMATORY, ANTIOXIDANT, CYTOTOXIC AND ANTIBACTERIAL STUDY OF CAPPARIS CARTILAGINEA DECNEFROM YEMEN

  • Bushra Abdulkarim Moharram Department of Pharmacognosy, Faculty of Pharmacy, Sana’a University, Sana’a, Yemen,
  • Hassan M. Al-mahbashi Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Sana`a University, Sanaa, Yemen,
  • Riyadh Saif Ali Department of Biochemistry and Molecular Biology, Faculty of Medicine, Sana’a University, Sana’a, Yemen
  • Faten Ali Aqlan Department of Pharmacognosy, Faculty of Pharmacy, Sana’a University, Sana’a, Yemen

Abstract

Objective: To investigate phytochemicals and biological activities of Capparis cartilaginea extracts.

Methods: The methanolic extracts of leaves, stem and twigs of C. cartilaginea were screened for their phytochemicals. The essential oil of the leaves was hydrodistilled by a Clevenger apparatus and analyzed by gas chromatography-mass spectrometry (GC-MS). The leaves extract of C. cartilaginea was evaluated for its anti-inflammatory effect, using formalin-induced paw edema. The leaves, stem and twig extracts were assessed for their antioxidant activity, using free radical scavenging assay, cytotoxic activity, using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and antibacterial activity, using the microdilution method.

Results: All extracts of C. cartilaginea contained alkaloids, carbohydrates, protein, coumarin, phytosterols, bitter principles, phenols and tannins. The essential oil of the leaves was mainly composed of isopropyl isothiocyanate (69.4%), butane,1-isothiocyanate (26.97%) and isobutyl isothiocyanate (3.26%). The leaves extract at doses of 200 and 400 mg/kg, significantly inhibited paw edema at the 3rd h (49.1%, 54.0%, respectively) and this effect was comparable to that of diclofenac (58.87%). The leaves extract showed the highest antioxidant activity with IC50 value of 91.71 µg/ml. The twigs extract exhibited the highest cytotoxic activity against human lung carcinoma (A549) with IC50 of 57.5 µg/ml. The leaves and stem extracts exhibited antibacterial activity against Staphylococcus aureus with minimum inhibitory concentration (MIC) of 5.0 mg/ml.

Conclusion: The leaves extract of C. cartilaginea is a potential source of bioactive compounds that could have a role in anti-inflammation. Twigs extract of the C. cartilaginea possesses a potential cytotoxic effect on human lung cell line.

Keywords: Capparis cartilaginea, Phytochemical, Essential oil, Anti-inflammation, Antioxidant cytotoxicity, Antibacterial

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Moharram, B. A., H. M. Al-mahbashi, R. S. Ali, and F. A. Aqlan. “PHYTOCHEMICAL, ANTI-INFLAMMATORY, ANTIOXIDANT, CYTOTOXIC AND ANTIBACTERIAL STUDY OF CAPPARIS CARTILAGINEA DECNEFROM YEMEN”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 6, June 2018, pp. 38-44, doi:10.22159/ijpps.2018v10i6.22905.
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