IMPACT OF PROLONGED REDUCED-PRESSURE CONDITION PRIOR TO PRECURSOR LABELING ON THE LABELING EFFICIENCY OF F-18 FLUOROCHOLINE SYNTHESIS

  • Hishar H. Centre for Diagnostic Nuclear Imaging, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Hanafi M. H. Centre for Diagnostic Nuclear Imaging, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Fathinul Fikri A. S. Centre for Diagnostic Nuclear Imaging, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Abstract

Objective: The goal of this preliminary work was to observe the impact of the prolonged reduced-pressure condition prior to labeling stage on the F-18 Fluorocholine labeling yield at the end of synthesis.

Methods: At this present work, the condition inside the reactor vial prior to labeling stage was manipulated. In the first technique of syntheses of F-18 Fluorocholine, the condition inside the reactor vial was set at 0 atmospheric pressure (0 atm) while in the second technique the condition inside the reactor was set at reduced-pressure (between-0.65 to-0.85 bars) with the delay time of 120 seconds. At the end of the synthesis, the impact of the prolonged reduced-pressure condition prior to precursor labeling was measured in terms of labeling yield of F-18 Fluorocholine.

Results: With the second technique, the labeling yield of F-18 Fluorocholine was elevated from 9.7% (the first technique) to 24.3%.

Conclusion: This preliminary work indicates that delay in a reduced-pressure condition prior to labeling step has greatly improved the labeling yield of F-18 Fluorocholine at the end of synthesis. Using this approach, the labeling yield of F-18 Fluorocholine was elevated from 7.5% to 24.3%.

Keywords: Azeotropic drying, F-18 Fluorocholine, Labeling yield, Reduced-pressure

Downloads

Download data is not yet available.

References

1. Hara T, Kosaka N, Kishi H. PET imaging of prostate cancer using carbon-11 choline. J Nucl Med 1998;39:990-5.
2. Yu KH, Park JH, Yang SD. Synthesis of [18F]Fluorocholine analogues as a potential imaging agent for PET studies. B Korean Chem Soc 2004;25:506-10.
3. Price DT, Coleman RE, Liao RP, Robertson CN, Polascik TJ, DeGrado TR. Comparison of [18F] Fluorocholine and [18F] fluorodeoxyglucose for positron emission tomography of androgen dependent and androgen independent prostate cancer. J Urol 2002;168:273-80.
4. Kobori O, Kirihara Y, Kosaka N, Hara T. Positron emission tomography of esophageal carcinoma using (11C)-choline and (18F) fluorodeoxyglucose: a novel method of preoperative lymph node staging. Cancer 1999;86:1638.
5. Hara T, Kosaka N, Shinoura N, Kondo T. PET imaging for brain tumour with [methyl-11C]choline. J Nucl Med 1997;38:842-7.
6. Kwee SA, DeGrado TR, Talbot JN, Gutman F, Coel MN. Cancer imaging with fluorine-18 labelled choline derivatives. Semin Nucl Med 2007;37:420-8.
7. Cimitan C, Bortolus R, Morassut S. [18F] Fluorocholine PET/CT imaging for the detection of recurrent prostate cancer at PSA relapse: experience in 100 consecutive patients. Eur J Nucl Med 2006;33:1387-98.
8. Kotzerke J, Gschwend JE, Neumaier B. PET for prostate cancer imaging still a quandary or the ultimate solution? J Nucl Med 2002;43:200-2.
9. Sperandeo A, Ficola U, Quartuccio N, Kitson SL, Mansi L, Cistaro A. Automated synthesis of [18F]Fluorocholine using a modified GE Tracer Lab module. JDIT 2014;1:49-58.
10. Sarrazin J, Philippon F, Tessier M. Usefulness of fluorine-18 positron emission tomography/computed tomography for identification of cardiovascular implantable electronic device infections. J Am Coll Cardiol 2012;59:1616-25.
11. Shao X, Hockley BG, Hoareau R, Schnau PL, Scott PJH. Fully automated preparation of C-11 choline and F-18 Fluormethyl-choline using TracerLab synthesis modules and facilitated quality control using analytical HPLC. Appl Radiat Isot 2011;69:403-9.
12. DeGrado TR, Baldwin SW, Wang S, Orr MD, Liao RP. Synthesis and evaluation of 18F-labeled choline analogs as oncologic PET tracers. J Nucl Med 2001;42:1805-14.
13. DeGrado TR, Coleman RE, Wang S. Synthesis and evaluation of 18F-labeled choline as an oncologic tracer for positron emission tomography: initial findings in prostate cancer. Cancer Res 2001;61:110-7.
14. Iwata R, Pascali C, Bogni A, Furumoto S, Terasaki K, Yanai K. [18F]fluoromethyl triflate, a novel and reactive [18F]fluoromethylating agent: preparation and application to the on-column preparation of [18F] Fluorocholine. Appl Radiat Isot 2002;57:347-52.
15. Kryza D, Tadino V, Filannino MA, Villeret G, Lemoucheux L. Fully automated [18F]Fluorocholine synthesis in the tracer lab MXFDG coincidence synthesizer. Nucl Med Biol 2008;35:255-60.
16. Lasne MC, Perrio C, Rouden J, Barre L, Roeda D, Dolle F, et al. Chemistry of ß±emitting compounds based on fluorine-18. Topp Curr Chem 2002;222:201-58.
17. Cai LS, Lu SY, Pike VW. Chemistry with [18F]Fluoride ion. Eur J Org Chem 2008;17:2853-73.
Statistics
379 Views | 569 Downloads
Citatons
How to Cite
H., H., H. M. H., and F. F. A. S. “IMPACT OF PROLONGED REDUCED-PRESSURE CONDITION PRIOR TO PRECURSOR LABELING ON THE LABELING EFFICIENCY OF F-18 FLUOROCHOLINE SYNTHESIS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 4, Apr. 2018, pp. 143-5, doi:10.22159/ijpps.2018v10i4.23057.
Section
Short Communication(s)