6-SHOGAOL RICH GINGER OLEORESIN LOADED MIXED MICELLES ENHANCES IN VITRO CYTOTOXICITY ON MCF-7 CELLS AND IN VIVO ANTICANCER ACTIVITY AGAINST DAL CELLS


Kiran Kemkar, Sathiyanarayanan L., Arulmozhi Sathiyanarayanan, Kakasaheb Mahadik

Abstract


Objective: Ginger oleoresin (GO) plays an important role on the attenuation of complications associated to the cancer which is attributed to 6-shogaol (6-SGL). The major challenge in using 6-SGL for therapeutic applications is its poor aqueous solubility, low stability in GI and low bioavailability. Considering the potent anticancer nature of 6-SGL and its synergistic activity with other constituents in GO, there is a need to develop a suitable drug delivery system.

Methods: Thus in the present study, 6-SGL rich GO (6-SRGO) was incorporated into mixed micelles using phospholipid (Soya Lecithin) as a carrier. The prepared 6-SRGO loaded mixed micelles (6-SRGO-LMM) were characterized physically and chemically using Fourier transform infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC) and further evaluated for stability study, in vitro release study, in vitro cytotoxicity study and in vivo anticancer activity in comparison with 6-SRGO.

Results: The composition such as, drug content (86.27±1.56), encapsulation efficiency (81.55±1.05) and particle size (356.11±4.07) were optimized using 32 factorial design. FTIR and DSC study confirm that the 6-SGL from 6-SRGO was entrapped in the core of phospholipid by self-assembly method to form mixed micelles. The 6-SRGO-LMM exhibited significant in vitro (GI50-23.2 μg/ml) and in vivo anticancer activity in comparison with 6-SRGO.

Conclusion: We have developed and investigated mixed micelles composed of phospholipids (soya lecithin S80) and SCH as an effective nanocarrier for the delivery of a natural lipophilic anticancer bioactive 6-SGL from 6-SRGO.


Keywords


6-shogaol, Mixed micelles, Factorial designs, Breast cancer

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About this article

Title

6-SHOGAOL RICH GINGER OLEORESIN LOADED MIXED MICELLES ENHANCES IN VITRO CYTOTOXICITY ON MCF-7 CELLS AND IN VIVO ANTICANCER ACTIVITY AGAINST DAL CELLS

Keywords

6-shogaol, Mixed micelles, Factorial designs, Breast cancer

DOI

10.22159/ijpps.2018v10i1.23077

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 1, 2018 Page: 160-168

Online ISSN

0975-1491

Authors & Affiliations

Kiran Kemkar
Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411 038, Maharashtra, India
India

Sathiyanarayanan L.
Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411 038, Maharashtra, India
India

Arulmozhi Sathiyanarayanan
Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411 038, Maharashtra, India
India

Kakasaheb Mahadik
Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411 038, Maharashtra, India
India


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