ANTI-INFLAMMATORY AND PROTECTIVE PROPERTIES OF ALOE VERA LEAF CRUDE GEL IN CARRAGEENAN INDUCED ACUTE INFLAMMATORY RAT MODELS
Keywords:Aloe vera, Carrageenan, Indomethacin, Medicinal plant, Paw edema, Pharmacology
Objectives: Current clinical treatment regimes for inflammatory diseases have different drawbacks including side effects of the drugs and the high cost of long term treatment. In the last few decades different promising herbal medicines have been explored for their anti-inflammatory and anti-rheumatic effects, but conclusive evidences are not available in the case of crude Aloe vera gel for its anti-inflammatory effects. The objective of the study was to document the protective and curative roles of orally administered and peritoneally injected crude wild Aloe vera gel in carrageenan-induced inflammation in a rat model.
Methods: Inflammation was induced by injecting 1% carrageenan in the left hind paw of Wistar albino rat. Crude, unprocessed Aloe vera gel was peritoneally injected and orally fed to experimental and control rat groups to investigate its effect on paw joint edema by measuring the paw circumference with vernier caliper. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cell viability assay was performed to investigate the cytotoxic effect of the gel.
Results: Paw edema was brought to near normal levels in the experimental groups after the treatment with crude Aloe vera gel. Orally fed gel showed no cytotoxicity on macrophages and spleenocytes. Protective property of crude Aloe gel was also evident in both the experiments.
Conclusion: Aloe vera crude gel has both protective and curative properties against inflammation.
Valiollah H, Seyed ES, Mojtaba H. Anti-inflammatory and analgesic properties of Heracleum persicum essential oil and hydroalcoholic extract in animal models. J Ethnopharmacol 2009;124:475-80.
Surjushe A, Vasani R, Saple DG. Aloe vera: a short review. Ind J Dermatol 2008;53:163-66.
Shelton RM. Aloe vera: its chemical and therapeutic properties. Int J Dermatol 1991;30(10): 679â€“83.
Eshun K, He Q. Aloe Vera: a valuable ingredient for the food, pharmaceutical and cosmetic industriesâ€”a review. Crit Rev Food Sci 2004;44(2): 91-6.
Tanaka M, Misawa E, Ito Y, Habara N, Nomaguchi K, Yamada M, et al. Identification of five phytosterols from Aloe vera gel as anti-diabetic compounds. Biol Pharm Bull 2006;29(7): 1418-22.
Hu Y, Xu J, Hu Q. Evaluation of Antioxidant Potential of Aloe vera (Aloe barbadensis Miller) Extracts. J Agr Food Chem 2003;51:7788-91.
Arunkumar S, Muthuselvam M. Analysis of phytochemical constituents and antimicrobial activities of Aloe vera L. against clinical pathogens. World J Agri Sci 2009;5(5): 572-6.
Davis RH, Leitner MG, Russo JM, Byrne ME. Wound healing. Oral and topical activity of Aloe vera. J Am Podiatr Med Assoc 1989;79(11): 559-62.
Gupta M, Mazumder UK, Gomathi P, Thamil Selvan V. Antiinflammatory evaluation of leaves of Plumeria acuminate. BMC Compl Altern Med 2006;6(36): 1-6.
Jain AK, Sharma P, Sikarwar MS. Evaluation of anti-inflammatory and anti-pyretic activity of total alcoholic extract of Croton sparsiflorus and its fractions. J Pharm Res 2010;3(5): 1149-51.
Winter CA, Risley EA, Nuss GW. Carrageenan-induced edema in the hind paws of the rats as an assay for anti-inflammatory drugs. Exp Biol Med 1962;111:544-47.
Shrinivasan K, Muruganandan S, Lal J. Evaluation of anti-inflammatory activity of Pongamia pinnata leaves in rats. J Ethnapharmacol 2001;78:151-7.
Foyet HS, Abdou BA, Ponka R, Asongalem AE, Kamtchouing P, Nastasa V. Effects of Hibiscus asper leaves extracts on carrageenan induced oedema and complete Freundâ€™s adjuvant-induced arthritis in rats. J Cell Anim Biol 2011;5(5): 69-75.
Shang HF, Cheng HC, Liang HJ, Liu HY, Liu SY, Hou WC. Immunostimulatory activities of yam tuber mucilages. Bot Stud 2007;48:63-70.
Dey P, Dutta S, Chaudhuri TK. Evaluation of erythrocytic membrane stabilizing activity and cytotoxic effect of the areal tubers of Dioscorea alata L of North-Eastern region of India. J Pharm Sci Innov 2013;2(3): 1-4.
Talmadge J, Chavez J, Jacobs L, Munger C, Chinnah T, Chow JT, et al. Fractionation of Aloe vera L. inner gel, purification and molecular profiling of activity. Int Immunopharmacol 2004;4:1757â€“1773.
Boudreau DM, Beland FA. An evaluation of the biological and toxicological properties of Aloe barbadensis (miller), Aloe Vera. J Env Sci Heal 2006;24:103â€“54.
Selvam C, Jachak SM. A cyclooxygenase (COX) inhibitory flavonoids from the seeds of Semecarpus anacardium. J Ethnopharmacol 2004;95:209-212.