EFFECT OF COMBINATION OF TWO PLANT EXTRACTS ON DIABETES MELLITUS


Jose Deepa, N. A. Aleykutty, Harindran Jyoti

Abstract


Objective: To investigate the anti-diabetic activity of combined ethanolic extracts (1:1mixture) of dry leaves of Syzygium cumini and Psidium guajava belonging to the family Myrtaceae as well as to compare the anti-diabetic activity of these plants by in vitro methods.

Methods: In vitro glucose uptake assay was performed on cultured L6 cell lines (rat myoblast cell line) and estimated the glucose uptake using high sensitivity glucose oxidase kit. In vitro alpha amylase inhibitory assay was performed on porcine alpha amylase and the absorbance was measured at 540 nm using a microplate reader. Acarbose was used as the standard in both the methods.

Results: At a concentration of 100µg/ml the percentage glucose uptake by the combined ethanolic extract (1:1 mixture) of Syzygium cumini and Psidium guajava leaves was 43.95 while for acarbose the corresponding value was 51.71. At 100 μg/ml the percentage of glucose uptake by Syzygium cumini and Psidium guajava was 27.62 and 22.17 respectively. The percentage inhibition of alpha amylase by the combined ethanolic extract (1:1 mixture) of Syzygium cumini and Psidium guajava leaves at a concentration of 1000 µg/ml was 36.51 and it was 29.26 for Syzygium cumini and 23.43 for Psidium guajava. For acarbose the percentage inhibition of alpha amylase was 73.82 at the concentration of 1000 µg/ml.

Conclusion: The combined extract of the leaves of the plants selected was found to be more effective than individual plant extracts against diabetes. The percentage glucose uptake of the combined extract was found to be closer to that of the standard drug acarbose. On comparison of two plants Syzygium cumini was found to be more active against diabetes than Psidium guajava. As the 1:1 mixture of the ethanolic extract is found to be more active, the combination of the two plants can be used to formulate drugs for treating diabetes.


Keywords


Glucose uptake assay, Alpha amylase inhibitory assay, Syzygium cumini and Psidium guajava

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References


Orwa C, Mutua A, Kindt R, Jamnadass R, Simons A. Syzygiumcuminii (L.) skeels Myrtaceae. Agroforestry database: a tree species Reference and Selection Guide; 2009;40:1-5.

Ayyanar M, Subash-Babu P. Syzygiumcumini (L.) skeels: a review of its phytochemical constituents and traditional uses. Asian Pac J Trop Biomed 2012;2:240–6.

Pradhan M. Phytochemistry, pharmacology and novel delivery applications of Syzygiumcumini. Int J Pharm Pharm Res 2016;7:659-75.

Shirur Dakappa Shruthi, Adhikari Roshan, Sanjay Sharma. A review on the medicinal plant Psidium Guajava Linn. (Myrtaceae). J Drug Delivery Ther 2013;3:162–8.

Sharmila C, Ranjith Kumar R, Chandar Shekar B. Psidium guajava: a novel plant in the synthesis of silver nanoparticles for biomedical applications. Asian J Pharm Clin Res 2018;11:341-5.

Thenmozhi P, Vijayalakshmi M. Knowledge on hypoglycemia among patients with diabetes mellitus. Asian J Pharm Clin Res 2018;11:236-9.

Swathiputta, Vijay C Ahedalawada. Dose dependent effect on hypoglycemic and antihyperglycemic activities of chloroform extract of Physalis minima in streptozotocin induced diabetic rats. Int J Curr Pharm Res 2014;6:61-5.

Olokoba AB, Obateru OA, Olokoba LB. Type 2 diabetes mellitus: a review of current trends. Oman Med J 2012;27:269–73.

World Health Organization. Global Report on Diabetes 2016;978:88.

Alberti KG, Zimmet PZ. WHO. Definition, diagnosis and classification of diabetes mellitus and its complications. part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 1998;15:539–53.

Gaby AD. Diabetes Mellitus. Nutr Med 2011;3:1081–105.

Patience OO, Estella UO, Philip FU. The search for new hypoglycemic agents from plants. Afr J Pharm Pharmacol 2014;8:292–303.

Gaikwad SB, Krishna Mohan G, Sandhya Rani M. Phytochemicals for diabetes management. Pharmaceutical Crops 2014;5(Suppl 1:M2):11–28.

Rosalie IO, Ekype EL. Antidiabetic potentials of common herbal plants and plant products: A glance. Int J Herb Med 2016;4:90–7.

Yap A, Nishiumi S, Yoshida KI, Ashida H. Rat L6 myotubes as an in vitro model system to study GLUT4-dependent glucose uptake stimulated by inositol derivatives. Cytotechnology 2007;55:103–8.

Wickramaratne MN, Punchihewa JC, Wickramaratne DBM. In vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina. BMC Complement Altern Med 2016;16:466-75.

DeFronzo RA, Tripathy D. Skeletal muscle insulin resistance is the primary defect in type 2 diabetes. Diabetes Care 2009;32 Suppl 2:157-63.

Safamansouri H, Nikan M, Amin G, Sarkhail P, Gohari AR, Kurepaz-Mahmoodabadi M, et al. α-Amylase inhibitory activity of some traditionally used medicinal species of Labiatae. J Diabetes Metab Disord 2014;13:114-23.

Gupta RC, Chang D, Nammi S, Bensoussan A, Bilinski K, Roufogalis BD. Interactions between antidiabetic drugs and herbs: an overview of mechanisms of action and clinical implications. Diabetol Metab Syndr 2017;9:59-65.




About this article

Title

EFFECT OF COMBINATION OF TWO PLANT EXTRACTS ON DIABETES MELLITUS

Keywords

Glucose uptake assay, Alpha amylase inhibitory assay, Syzygium cumini and Psidium guajava

DOI

10.22159/ijpps.2018v10i4.24100

Date

01-04-2018

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 4, 2018 Page: 49-52

Online ISSN

0975-1491

Authors & Affiliations

Jose Deepa
Vice Principal, Nirmala College of Pharmacy, Muvattupuzha, Kerala, India
India

N. A. Aleykutty
Principal, Caritas College of Pharmacy, Kottayam, Kerala, India
India

Harindran Jyoti
Principal and Head of the Research Centre, Dept. of Pharmaceutical Sciences, Cheruvandoor, Kottayam, Kerala, India
India


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