PREVENTION OF PEPTIC ULCERS BY CURCUMIN IN CHEMICALLY INDUCED OSTEOARTHRITIS
Objective: The present study was carried out to investigate the role of curcumin in osteoarthritis when it is used as an adjuvant to diclofenac sodium.
Methods: Osteoarthritis (OA) was induced by administering nalidixic acid 400 mg/kg. Animals (Female rats) were divided into 5 groups each containing 6 animals. Group I was considered as control, group II in which the animals were induced with osteoarthritis with nalidixic acid and were given no treatment. Group III in which osteoarthritis induced animals were treated with diclofenac sodium by the oral route. Group IV osteoarthritis induced animals were treated with the combination of diclofenac sodium and curcumin and group V animals were pre-treated with curcumin and then induced with osteoarthritis. Parameters like ulcer area, ulcer index, free acidity, total acidity, the volume of gastric juice were estimated. Histopathological studies were also carried out.
Results: The data of our study shows that nalidixic acid has not shown much effect on the gastric parameters in group II animals. The ulcer index, free acidity, total acidity and gastric juice volume were increased significantly (p<0.001) in group III and decreased in group IV animals (p<0.05) when compared to group I control animals. Group V animals pretreated with curcumin have shown fewer incidences of gastric ulcers and other ulcerative parameters non significantly. Histopathology also suggests a low incidence of ulcers in group IV and group V.
Conclusion: It demonstrates that curcumin when used along with the conventional NSAIDs as an adjuvant therapy, has a role in treating osteoarthritis effectively.
2. Kellgren JH, Moore R. Generalized osteoarthritis and Heberden's nodes. Br Med J 1952;1:181.
3. Uivarosi V. Metal complexes of quinolone antibiotics and their applications: an update. Molecules 2013;18:11153-97.
4. Shay H, Kamarow SA, Fels SS, Mersanze D, Guenstein M, Siplet HA. A simple method for the uniform production of gastric ulceration in the rat. Gastroenterol 1945;5:43-61.
5. Sener G, Paskaloglu K, Ayanoglu-dÃ¼lger G. Protective effect of increasing doses of famotidine, omeprazole, lansoprazole, and melatonin against ethanol-induced gastric damage in rats. Indian J Pharmacol 2004;36:171â€“4.
6. Turnberg LA. Gastric mucosal defence mechanisms: a brief review. Scandinavian J Gastroenter 1985;20:37-40.
7. Hoy B, Brandstetter H, Wessler S. The stability and activity of recombinant Helicobacter pylori HtrA under stress conditions. J Basic Microbiol 2013;53:402-9.
8. Castro GA, Carvalho JE, Tinti SV, Possenti A, Sgarbieri VC. Anti-ulcerogenic effect of a whey protein isolate and collagen hydrolysates against ethanol ulcerative lesions on oral administration to rats. J Med Food 2010;13:83-90.
9. Matsui H, Shimokawa O, Kaneko T, Nagano Y, Rai K, Hyodo I. The pathophysiology of nonsteroidal antiinflammatory drug (NSAID) induced mucosal injuries in stomach and small intestine. J Clin Biochem Nutr 2011;48:107-11.
10. Bjarnason I, Hayllar J. Early pathogenic events in NSAID-induced gastrointestinal damage. Ital J Gastroenterol 1996;28 Suppl 4:19â€“22.
11. Higuchi K, Umegaki E, Watanabe T. Present status and strategy of NSAIDs-induced small bowel injury. J Gastroenterol 2009; 44:879â€“88.
12. Thong-Ngam D, Choochuai S, Patumraj S, Chayanupatkul M, Klaikeaw N. Curcumin prevents indomethacin-induced gastropathy in rats. World J Gastroenterol 2012;18:1479â€“84.
13. Yadav SK, Sah AK, Jha RK, Sah P, Shah DK. Turmeric (curcumin) remedies gastroprotective action. Pharmacogn Rev 2013;7:42â€“6.
14. Balogun E, Hoque M, Gong P. Curcumin activates the haem oxygenase-1 gene via regulation of Nrf2 and the antioxidant-responsive element. Biochem J 2003;371:887â€“95.
15. Blandine Poulet, Frank Beier. Targeting oxidative stress to reduce osteoarthritis. Arthritis Res Ther 2016;18:32.
16. Youn GS, Kwon DJ, Ju SM, Choi SY, Park J. Curcumin ameliorates TNF-Î±-induced ICAM-1 expression and subsequent THP-1 adhesiveness via the induction of heme oxygenase-1 in the HaCaT cells. BMB Rep 2013;46:410-5.
17. Rogers NM, Stephenson MD, Kitching AR, Horowitz JD, Coates PT. Amelioration of renal ischaemia-reperfusion injury by liposomal delivery of curcumin to renal tubular epithelial and antigen-presenting cells. Br J Pharmacol 2012;166:194â€“209.
18. Trujillo J, Chirino YI, Molina-Jijon E, Anderica-Romero AC, Tapia E, Pedraza-Chaverri J. Renoprotective effect of the antioxidant curcumin: recent findings. Redox Biol 2013;1:448â€“56.