ANTI-OXIDATIVE, ANTI-INFLAMMATORY AND ANTI-ATHEROSCLEROTIC EFFECT OF TAURINE ON HYPERCHOLESTEROLEMIA INDUCED ATHEROSCLEROTIC RATS
DOI:
https://doi.org/10.22159/ijpps.2018v10i3.24372Keywords:
Hypercholesterolemia, Anti-inflammatory, Taurine, Anti-atherosclerotic, AntioxidantsAbstract
Objective: The present study evaluates the antioxidant, anti-inflammatory and anti-atherosclerotic potency of taurine (2-amino ethane sulfonic acid) when administered orally to hypercholesterolemia induced atherosclerotic rats.
Methods: The experimental atherosclerosis was induced by feeding rats with an atherogenic diet comprising of the normal rat chow supplemented with 4 % cholesterol, 1 % cholic acid and 0.5 % thiouracil (CCT diet) for 20 d. Treatment with atorvastatin (10 mg/kg body weight) and taurine (2 % in drinking water) was given to atherosclerotic rats to study antioxidant enzymes (superoxide dismutase, catalase, glutathione-S-transferase), lipid peroxidation in liver, glutathione reductase and protein carbonyl content, extent of DNA damage using the alkaline comet assay, assaying pro-inflammatory cytokines and quantifying atherosclerotic lesions.
Results: Oral supplementation of 2 % taurine to hypercholesterolemic rats modulated antioxidant status and significantly reduced malondialdehyde (MDA) content (P<0.05). The extent of DNA damage was also significantly reduced as observed by a reduction in the comet tail index (P<0.05). Taurine exhibited anti-inflammatory activity by significantly inhibiting TNF-α (tumor necrosis factor) and IL-1α (inter leukine) and also inhibited atherosclerotic lesions by clearing lipid deposits on the intimal surface of the rat aorta.
Conclusion: Oral administration of taurine to rats showed antioxidant and anti-inflammatory activity by modulating oxidants in favor of reducing oxidative stress and also showed anti-atherosclerotic activity in hypercholesterolemia-induced atherosclerosis.
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Raida K, Nizar A, Barakat S. The effect of Crataegus aronica aqueous extract in rabbits fed with high cholesterol diet. Eur J Sci Res 2008;22:352-60.
Lee MK, Park YB, Moon SS. Hypocholesterolemic and antioxidant properties of 3-(4hydroxyl) propanoic acid derivatives in high-cholesterol-fed rats. Chem Biol Interactions 2007;1:9-19.
Vogiatzi G, Tousoulis D, Stefanadis C. The role of oxidative stress in atherosclerosis. Hellenic J Cardiol 2009;50:402-9.
Aruoma OI, Halliwell B, Hoey BM, Butler J. The antioxidant action of taurine, hypotaurine and their metabolic precursors. Biochem J 1988;256:251-5.
Kritchevsky D. Experimental atherosclerosis in primates and other species. Annals New York Academy Sci 1969;162:80-8.
Srinivas M, Annapurna A, Reddy YN. Anti-atherosclerotic effect of atorvastatin and clopidogrel alone and in combination in rats. Indian J Exp Biol 2008;46:698-703.
Nandhini AT, Balakrishnan SD, Anuradha CV. The response of the liver antioxidant system to taurine in rats fed high fructose diet. Indian J Exp Biol 2002;40:1016-9.
Varley H. Practical Biochemistry. 4th ed. New Delhi (Indian): Amold Heinemann Publishers; 1976.
Tukozkan N, Erdamar H, Seven I. Measurement of total malondialdehyde in plasma and tissue by high-performance liquid chromatography and thiobarbituric acid assay. Firat Tip Dergisi 2006;11:88-92.
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469-74.
Aebi H. Catalase. In: Bergmeyer HU. editor. Methods in an enzymatic assay. New York: Academic Press; 1983. p. 276-86.
Habig WH, Pabst MJ, Jacoby WB. Glutathione-S-transferases, the first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130-9.
Beutler E, Duron O, Kelly BM. Improved methods for the determination of blood glutathione. J Lab Clin Med 1963;61:882-8.
Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz A, et al. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 1990;186:464-78.
Gandhi NM, Nair CKK. Radiation protection by Terminalia chebula: some mechanistic aspects. Mol Cell Biochem 2005;277:43-8.
Benkovic V, Knezevic AH, Dikic D, Lisicic D, Orsolic N, Basic I, et al. Radioprotective effects of propolis and quercetin in gamma-irradiated mice evaluated by alkaline comet assay. Phytomedicine 2008;15:85-8.
Holman RL, McGill HC Jr, Strong JP, Geer JC. Techniques for studying atherosclerotic lesions. Lab Invest 1958;7:42-7.
Dominiczak MH. Hyperlipidemia and cardiovascular disease. Curr Opin Lipidol 1998;9:609-11.
Esterbauer H, Wag G, Puhl H. Lipid peroxidation and its role in atherosclerosis. Brit Med Bull 1993;49,566-76.
Fong LG, Steiberg D. Oxidatively modified low-density lipoproteins: A potential role in recruitment and retention of monocyte/macrophages during atherogenesis. Proc Natl Acad Sci USA 1987;84:2995-8.
Kumar N, Kar A. Ameliorating effects of pyrroloquinoline quinone (PQQ) on PTU induced oxidative damage in mice kidney. Asian J Pharm Clin Res 2014;7:121-6.
Mishra R, Karmarkar SM, Bhagwat AM. Preliminary dose dependent study on antihyperlipidemic activity of Hibiscus rosa sinensis Linn leaves on triton WR 1339 induced hyperlipidemic mice model. Asian J Pharm Clin Res 2011;4:100-2.
Cui BK, Liu S, Lin XJ, Wang J, Li SH, Wang QB et al. Effects of Lycium barbarum aqueous and ethanol extracts on high fat-diet induced oxidative stress in rat liver tissue. Molecules 2011;16:9116-28.
Dalle-Donne I, Rossi R, Colombo R, Giustarini D, Milzani A. Biomarkers of oxidative damage in human disease. Clin Chem 2006;52:601-23.
Stadtman ER, Levine RL. Free radical-mediated oxidation of free amino acids and amino acid residues in proteins. Amino Acids 2003;25:207-18.
Collins AR, Dusinska M, Gedik CM, Stetina R. Oxidative damage to DNA: do we have a reliable biomarker? Environ Health Perspect 1996;04 Suppl 3:465-69.
Gedik CM, Wood SG, Collins AR. Measuring oxidative damage to DNA; HPLC and the comet assay compared. Free Radic Res 1998;29:609-15.
Harangi M, Remenyik E, Seres I, Varga Z, Katona E, Paragh G. Determination of DNA damage induced by oxidative stress in hyperlipidemic patients. Mutat Res 2001;513:17-25.
Andreassi MG. Coronary atherosclerosis and somatic mutations: an overview of the contributive factors for oxidative DNA damage. Mutat Res 2003;543:67-86.
Kamesh V, Sumathi T. Effect of Bacopa monniera Linn. in attenuating hepatic oxidative stress in hypercholesterolemic induced rats. Asian J Pharm Clin Res 2012;5:90-5.
Morrow DA, Ridker PM. C-reactive protein, inflammation, and coronary risk. Med Clin North Am 2000;84:149-61.
Berg AH, Scherer PE. Adipose tissue, inflammation, and cardiovascular disease. Circ Res 2005;96:939-49.
Barnes PJ, Karin M. Nuclear factor-kappa B: a pivotal transcription factor in chronic inflammatory diseases. N Engl J Med 1997;336:1066-71.
Hajra L, Evans AI, Chen M. The NF-kappa B signal transduction pathway in aortic endothelial cells is primed for activation in regions predisposed to atherosclerotic lesion formation. Proc Natl Acad Sci USA 2000;97:9052-7.
Sethupathy S, Elanchezhiyan C, Vasudevan K Kim H, Jeon H, Kong H, Yang Y, Choi B, Kim YM, et al. A molecular mechanism for the anti-inflammatory effect of taurine-conjugated 5-aminosalicylic acid in inflamed colo. Mol Pharmacol 2006;69:1405-12.
Rajagopal G. Antiatherogenic effect of taurine in high fat diet fed rats. Indian J Exp Biol 2002;40:1169-72.
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