ANTI-OXIDATIVE, ANTI-INFLAMMATORY AND ANTI-ATHEROSCLEROTIC EFFECT OF TAURINE ON HYPERCHOLESTEROLEMIA INDUCED ATHEROSCLEROTIC RATS
Keywords:Hypercholesterolemia, Anti-inflammatory, Taurine, Anti-atherosclerotic, Antioxidants
Objective: The present study evaluates the antioxidant, anti-inflammatory and anti-atherosclerotic potency of taurine (2-amino ethane sulfonic acid) when administered orally to hypercholesterolemia induced atherosclerotic rats.
Methods: The experimental atherosclerosis was induced by feeding rats with an atherogenic diet comprising of the normal rat chow supplemented with 4 % cholesterol, 1 % cholic acid and 0.5 % thiouracil (CCT diet) for 20 d. Treatment with atorvastatin (10 mg/kg body weight) and taurine (2 % in drinking water) was given to atherosclerotic rats to study antioxidant enzymes (superoxide dismutase, catalase, glutathione-S-transferase), lipid peroxidation in liver, glutathione reductase and protein carbonyl content, extent of DNA damage using the alkaline comet assay, assaying pro-inflammatory cytokines and quantifying atherosclerotic lesions.
Results: Oral supplementation of 2 % taurine to hypercholesterolemic rats modulated antioxidant status and significantly reduced malondialdehyde (MDA) content (P<0.05). The extent of DNA damage was also significantly reduced as observed by a reduction in the comet tail index (P<0.05). Taurine exhibited anti-inflammatory activity by significantly inhibiting TNF-Î± (tumor necrosis factor) and IL-1Î± (inter leukine) and also inhibited atherosclerotic lesions by clearing lipid deposits on the intimal surface of the rat aorta.
Conclusion: Oral administration of taurine to rats showed antioxidant and anti-inflammatory activity by modulating oxidants in favor of reducing oxidative stress and also showed anti-atherosclerotic activity in hypercholesterolemia-induced atherosclerosis.
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