• Sathya Rengasamy Centre for Research and Development, PRIST University, Tamil Nadu, India
  • Ushadevi Thangaprakasam Centre for Research and Development, PRIST University, Tamil Nadu, India



Streptomyces, Isolation, Screening, Quantitative determination, Amylase


Objective: This study was aimed to isolate potent amylase producing Streptomyces from the marine source.

Methods: Soil samples were collected from less explored mangrove regions of Muthupet, Tamilnadu. Isolation of Streptomyces was performed by serial dilution plate technique using starch casein agar (SCA) (pH 7.2 and temp 28 °C). Morphological and biochemical characteristics were studied using Bergey's manual of systematic bacteriology. Preliminary screening and quantification of amylase activities were analysed in selected Streptomyces isolates by starch agar plate and dinitrosalicylic acid (DNS) method respectively.

Results: Totally 65 isolates were separated from the marine soil. Among them, 23 strains showed different morphological features. These strains were subjected to amylase activity. Eight Streptomyces isolates (S1-S8) exhibited positive for amylase activity. The zone of clearance was exhibited in the range of diameters between 4-20 mm. Fermentation was prompted with inorganic salt starch agar, international Streptomyces project (ISP-4) media at 28 °C and incubated in an orbital shaker at 250 rpm for 96 h (pH 7.5). The quantitative estimation of amylase activity was exhibited selected eight isolates in the range between 2.4±0.002-5.9±0.005 (U/ml). The Streptomyces species S4, S5 and S6 exhibited strong amylase activity in both qualitative and quantitative level.

Conclusion: This work motivating the amylase producing Streptomyces are originated in mangroves and it proved Streptomyces sp. S6 has a more efficient source of amylase production.


Download data is not yet available.


Rajagopalan G, Krishnan C. Alpha amylase production from catabolic depressed Bacillus subtilis KCC103 utilizing sugarcane bagasse hydrolysate. Bioresour Technol 2008;99:3044-50.

Reddy NS, Nimmagadda A, Sambasiva Rao KRS. An overview of the microbial amylase family. Afr J Biotechnol 2003;2:645-8.

Gupta R, Gigras P, Mohapatra H, Goswami VK, Chauhan B. Microbial amylases a biotechnological perspective. Process Biochem 2003;38:1599-16.

Abdullah R, Shaheen N, Iqtedar M, Naz S, Iftikhar T. Optimization of cultural conditions for the production of alpha-amylase by Aspergillus niger (BTM-26) in solid state fermentation. Pak J Bot 2014;46:1071-8.

Pandey A, Soccol CR, Mitchell D. New developments in solid state fermentation: Bioprocess and products. Process Chem 2000;35:1153-69.

Konsoula Z, Liakopoulou Kyriakides M. Co-production of α-amylase and β-galactosidase by Bacillus subtilis in complex organic substrates. Bioresour Technol 2007;98:150-7.

Bull AT, Stach JEM, Ward AC, Goodfellow M. Marine actinobacteria; perspectives, challenges, future directions. Antonie Van Leeuwehoek 2005;87:65-79.

Sreejetha M, Divya, Veena S, Kokati Venkata BR. The bioactive potential of Streptomyces variabilis-DV-35 isolated from Thottada Marine sediments, Kannur, Kerala. Asian J Pharm Clin Res 2016;9:67-71.

Ruan CY, Zhang L, Ye WW, Srivibool R, Duangmal K, Pathom-aree W, et al. Streptomyces ferrugineus sp. nov., isolated from mangrove soil in Thailand. Antonie Van Leeuwenhoek 2015;107:39-45.

Vigal T, Gil JF, Daza A, Garcia-Gonzalez MD, Martin JF. Cloning characterization and expression of an alpha-amylase gene from Streptomyces griseus IMRU 3570. Mol Gen Genet 1991;225:278-88.

Chakraborty S, Raut G, Khopade A, Mahadik K, Kokare C. Study on calcium ion dependent amylase from haloalkaliphilic marine Streptomyces strain A3. Indian J Biotechnol 2012;11:427-37.

Acharyabhatta A, Kandula SK, Terli R. Taxonomy and polyphasic characterization of alkaline amylase producing marine Streptomyces rochei BTSS 1001. Int J Microbiol 2013;1-8.

Van Der Maarel JM, Van Der Veen B, Uitdehaag JC, Leemhuis H, Dijkhuizen L. Properties and applications of starch-converting enzymes of the alfa amylase family. J Bacteriol 2002;94:137-55.

Shafiei M, Ziaee AA, Amoozegar MA. Purification and characterization of an organic solvent tolerant halophilic a-amylase from the moderately halophilic Nesterenkonia sp. strain F. J Ind Microbiol Biotechnol 2011;38:275-81.

Jerry Reen F, Gutierrez-Barranquero JA, Dobson ADW, Adams C, Gara FO. Emerging concepts promising new Horizons for marine biodiversity and synthetic biology. Mar Drug 2015;13:2924-54.

Whitehurst RJ, Oort MV. Second Edition. Enzymes in Food Technology. Wiley Blackwell Pub; 2010. p. 44-5.

Sarrouh B, Santos TM, Miyoshi A, Dias R, Azevedo V. Up-To-date insight on industrial enzymes applications and global market. J Bioprocess Biotech 2012;4:1-10.

Hmidet N, El-Hadj Ali N, Haddar A, Kanoun S, Nasri M. Alkaline proteases and thermostable alpha-amylase co-produced by Bacillus licheniformis NH1: characterization and potential application as a detergent additive. Biochem Eng 2009;47:71-9.

Oyeleke SB, Auta SH, Egwin EC. Production and characterization of amylase produced by Bacillus megaterium isolated from a local yam peel dumpsite in Minna, Niger state. J Microbiol 2010;2:88-92.

Kuster E, Williams ST. Production of hydrogen sulphide by Streptomyces and methods for its detection. Appl Microbiol 1964;12:46-52.

Shiriling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:312-40.

Locci R. Streptomyces and related genera. Bergey’s manual of systematic bacteriology. In. Baltimore: Williams and Wilkins Company; 1989. p. 2451-508.

Kawato M, Shinolue R. A simple technique for the microscopic observation, memoirs of the Osaka University. Liberal Arts Education 1959. p. 114.

Williams ST, Goodfellow M, Alderso G, Wellkington EMH, Sneath PHA, Sackins MJ, et al. Numerical classification of Streptomyces and related genera. J Gen Microbiol 1983;129:1743-813.

Hankin L, Anagnostaskis SL. The use of solid media for detection of enzyme production by fungi. Mycologia 1975;67:597-607.

Miller GL. Use of dinitrosalicyclic acid for determining reducing sugars. Anal Chem 1959;31:426-8.

He X, Yu Ou H, Yu Q, Zhou X, Wu J, Liang J, et al. Analysis of a genome island housing genes for DNAS-modification system in Streptomyces lividans 66 and its counterparts in other distantly related bacteria. Mol Microbiol 2007;64:1034-48.

Stach JCM, Maldonado LA, Ward AC, Bull AT, Goodfellow M. Williamsia maris sp. nov., a novel actinomycetes isolated from the Sea of Japan. Int J Syst Evol Microbiol 2004;54:191-4.

Mu W, Wang X, Xue Q, Jiang B, Zhang T, Miao M, et al. Characterization of a thermostable glucose isomerase with an acidic pH optimum from Acidothermus cellulolyticus. Food Res Int 2012;47:364-7.

Li MY, Xiao Q, Pan JY, Wu J. Natural products from semi-mangrove flora: source, chemistry and bioactivities. Nat Prod Rep 2009;26:281-98.

Vijayakumar R, Murugesan S, Panneerselvam A. Isolation, characterization and antimicrobial activity of actinobacteria from point calimere coastal region, East coast of India. Int Res J Pharm 2010;1:358-65.

Prabhahar C, Saleshrani K, Enbarasan R. Isolation and characterization of marine actinomycetes from muthuppettai mangroves, Tamilnadu, India. Int J Rec Sci Res 2014;5:906-10.

Ser HL, Zainal N, Palanisamy UD, Goh BH, Yi WF, Chan KG, et al. Streptomyces gilvigriseus sp. nov., a novel actinobacterium isolated from mangrove forest soil. Antonie Van Leeuwenhoek 2015;107:1369-78.

Goodfellow M, Stanton LJ, Simpson KE, Minnikin DE. Numerical and chemical classification of Actinoplanes and some related actinomycetes. J Gen Microbiol 1990;136:19-36.

Nonomura H. Key for isolation and identification of 458 species of the Streptomycetes included in ISP. J Ferment Technol 1974;52:78-92.

Dash BK, Rahman MM, Sarker PK. Molecular identification of a newly isolated Bacillus subtilis BI19 and optimization of production conditions for enhanced production of extracellular amylase. BioMed Res Int 2015:1-9. Doi:10.1155/2015/859805.

Gopinath SCB, Anbu P, Md Arshad MK, Lakshmipriya T, Voon CH, Hashim U, et al. Biotechnological process in microbial amylase production. BioMed Res Int 2017;1-9.

Syed DG, Agasar D, Pandey A. Production and partial purification of amylase from a novel isolate Streptomyces gulbargensis. J Ind microbiol Biotechnol 2009;36:189-94.

Hwang SY, Nakashima K, Okai N, Okazaki F, Miyake M, Harazono K, et al. Thermal stability and starch degradation profile of amylase from Streptomyces avermitilis. Biosci Biotechnol Biochem 2013;7:2449-53.

Moghbeli M, Noshiri H. Isolation of a native Bacillus licheniformis amylse producer from the hot source of semnan. J Microbial World 2009;2:155-60.

Kafilzadeh F, Dehdari F. Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in South of Iran. Egypt J Aquat Res 2015;41:197-201.

Khusro A, Barathikannan K, Aarti C, Agastian P. Optimizaiton of thermo-alkali stable amylase production and biomass yield from Bacillus sp. Under submerged cultivation. Fermentation 2017;3:73.

Singh S, Singh S, Bali V, Sharma L, Mangla J. Production of fungal amylases using cheap, readily available agriresidues, for potential application in Textile industry. BioMed Res Int 2014;214-9.

Simiar AA, Qureshi AS, Khushk I, Haider Ali C, Lashari S, Bhutto MA, et al. Production and partial characterization of amylase enzyme from Bacillus sp. BCC 01-50 and potential applications. BioMed Res Int 2017:9.

Krishma M, Radhathirumalaiarasu S. Isolation, identification and optimization of alkaline amylase production from Bacillus cereus using agro-industrial wastes. Int J Currt Microbiol Appl Sci 2017;6:20-8.

Naragani K, Muvva V, Munaganti R, Hima Bindu B. Studies on optimization of amylase production by Streptomyces cheonanensis VUK-A isolated from mangrove habitats. J Adv Biol Biotechnol 2015;3:165-72.



How to Cite

Rengasamy, S., and U. Thangaprakasam. “ISOLATION, SCREENING AND DETERMINATION OF Α-AMYLASE ACTIVITY FROM MARINE STREPTOMYCES SPECIES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 10, no. 4, Apr. 2018, pp. 122-7, doi:10.22159/ijpps.2018v10i4.24447.



Original Article(s)