• Amresh Kumar Yadav Department of Botany, Patna University, Patna-800005, Bihar, India
  • Sanjeev Kumar Ambasta Department of Botany, Patna University, Patna-800005, Bihar, India
  • Surendra Kumar Prasad Department of Botany, Patna University, Patna-800005, Bihar, India
  • M. P. Trivedi Department of Botany, Patna University, Patna-800005, Bihar, India


Objective: To evaluate the antibacterial property of crude, aqueous and organic solvent extract from leaf, stem and root parts of two different var. of Catharanthus roseus (i.e. “rosea†and “albaâ€) under in vitro conditions on various human pathogenic bacteria.

Methods: Antibacterial activity of crude (fresh), aqueous, ethanolic, methanolic and equimolar (1:1) mixture of ethanolic dried leaf extract of variety “rosea†and “alba†was evaluated against various pathogenic bacteria viz. Bacillus subtilis, Escherichia coli and Staphylococcus aureus by disk diffusion method under in vitro conditions.

Results: Gram-positive bacteria were found to be more susceptible than Gram-negative. Dried extracts of root, stem and leaf of C. roseus var. “rosea†and “alba†plants showed maximum antibacterial potency against all the test microorganisms. The equimolar mixture of ethanolic dried leaf extracts of species “rosea†and “alba†exhibited the maximum zone of inhibition against B. subtilis, E. coli and S. aureus as compare to extract prepared from individual parts. The findings of the ethanolic mixture of dried leaves of the two varieties on the tested bactera confirm that the effect is potentiating which may be synergistic or additive.

Conclusion: From the findings, it could be inferred that C. roseus var. “rosea†and “alba†could be efficiently used in the development of new life-saving drugs against bacterial pathogens.


Keywords: Catharanthus roseus, Pathogenic bacteria (B. subtilis, E. coli, S. aureus), In vitro, Zone of Inhibition, Drug resistance


Download data is not yet available.


1. Harbottle H, Thakur S, Zhao S, White DG. Genetics of antimicrobial resistance. Anim Biotechnol 2006;17:111–24.
2. Newman DJ, Cragg GM, Snader KM. Natural products as a source of new drug over the period 1981-2002. J Nat Prod 2003;6:1022-37.
3. Ambasta SK, Yadav AK, Sinha UK. Evaluation of anticlastogenic potential of Cinnamomum cassia bark extract against arsenic genotoxicity by using micronucleus assay in Mus musculus.
4. S Amrita, D Praveen. Screening of phytochemical and antimicrobial potential of the extract Vetiver zizanoides and Phragmites karka against clinical isolates. Int J Appl Pharm 2015;7:22-4.
5. Coates A, Hu Y, Bax R, Page C. The future challenges facing the development of new antimicrobial drugs. Nat Rev Drug Discovery 2002;1:895–910.
6. Nisbet LJ, Moore M. Wild natural products remains an important source of drug research for the future. Curr Opin Biotechnol 1997;8:708-12.
7. Braga LC, Leite AAM, Xavier KGS. Synergic interaction between Pomegranate extracts and antibiotics against Staphylococcus aureus. Can J Microbiol 2005;51:541–7.
8. Das S, Borah M, Ahmed S. Antibacterial activity of the ethanolic extract of leaves of Citrus maximum (Burm.) merr. on Escherichia coli and Pseudomonas aeruginosa. Asian J Pharm Clin Res 2017;6:136-9.
9. Ambasta SK, Yadav AK, Sinha UK. Evaluation of anticlastogenic potential of Cinnamomum cassia bark extract against arsenic genotoxicity by using micronucleus assay in Mus musculus caudal erythrocytes. Asian J Pharm Clin Res 2017;10:150-2.
10. Nair R, Chanda S. Activity of some medicinal plants against certain pathogenic bacterial strains. Indian J Pharmacol 2006;38:142–4.
11. Walsh SE, Maillard JY, Russel AD. Activity and mechanism of action of selected biocidal agents on gram-positive and gram-negative bacteria. J Appl Microbiol 2003;94:240–7.
12. Vander Heijden R, Jacobs DI, Snoeijer W, Hallard D, Verpoorte R. The Catharanthus alkaloids: pharmacognosy and biotechnology. Curr Med Chem 2004;11:607–28.
13. Magnotta M, Murata J, Chen J, De Luca V. Identification of a low vindoline accumulating cultivar of Catharanthus roseus(L.) G. Don by alkaloid and enzymatic profiling. Phytochemistry 2006;31:1758-64.
14. Paikara D, Pandey B, Singh S. Phytochemical analysis and antimicrobial activity of Catharanthus roseus. Indian J Sci Res 2017;12:124-7.
15. Almagro L, Fernandez-Perez F, Pedreno MA. Indole alkaloids from Catharanthus roseus: bioproduction and their effect on human health. Molecules 2015;20:2973–3000.
16. Abdulkarim A, Sadiq Y. Evaluation of five medicinal plants used in diarrhea treatment in Nigeria. J Ethnopharmacol 2005;101: 27-30.
17. Appidi JR, Grierson DS Afolayan. The ethnobotanical study of plants used for the treatment of diarrhea treatment in the Eastern Cape South Africa. Pakistan J Bio Sci 2008;11:1961-3.
18. Prakash V, Timasheff SN. Mechanism of interaction of vinca alkaloids with tubulin: Catharanthine and vindoline. Biochem 1991;30:873–80.
19. Muhammad LR, Muhammad N, Tanveer A, Baqir SN. Antimicrobial activity of different extracts of Catharanthus roseus. Clin Exp Med J 2009;3:81-5.
20. Chattopadhyay RR, Banerjee RN, Sarkar SK, Ganguly S, Basu TK. Anti-inflammatory and acute toxicity studies with the leaves of Vinca rosea Linn in experimental animals. Ind J Physiol Pharmacol 1992;36:291-2.
21. K Rajbir, A Saroj. Alkaloids-important therapeutic secondary metabolites of plant origin. J Crit Rev 2015;2:1-8.
22. El-Sayed M, Verpoorte R. Methyl jasmonate accelerates catabolism of monoterpenoid indole alkaloids in Catharanthus roseus during leaf processing. Fitoterapia 2005;76:83-90.
23. Haines HH. The Botany of Bihar and Orissa. 2nd ed. Gouranga press private Ltd; 1961.
24. Bergey DH, Whitman WB, Goodfellow, Kampfer P, Busse HJ. Bergey’s manual of systemic bacteriology. 2nd ed. New York: Springer; 2012.
25. Heinzmann S, Entian KD, Stein T. Engineering bacillus subtilis ATCC6633 for improved production of the antibiotic subtilin. Appl Microbiol Biotechnol 2006;69:532-6.
26. Islam MA, Heuvelink AE, de Boer E, Sturm PD, Beumer RR, Zwietering MH, et al. Shiga toxin producing Escherichia coli isolated from patients with diarrhea in Bangladesh. J Med Microbiol 2007;56:1–6.
27. Lowy FD. Staphylococcus aureus infections. N Engl J Med 1998;339:520–32.
28. Ferreres F, Pereira DM, Valentao P, Andrade PB, Seabra RM, Sottomayor M. New phenolic compounds and antioxidant potential of Catharanthus roseus. J Agric Food Chem 2008;56:9967–74.
29. Mustafa RN, Verpoorte R. Phenolic compounds in Catharanthus roseus. Phytochem Rev 2007;6:243–58.
30. Kumari K, Gupta S. Phytopotential of Catharanthus roseus L. (G.) Don var. “rosea” and “alba” against various pathogenic microbes in vitro. Int J Res Pure Appl Microbiol 2013;3:77-82.
31. Govindasamy, Srinivasan R. In vitro antibacterial activity and phytochemical analysis of Catharanthus roseus (Linn.) G. Don. Asian Pacific J Trop Biomed 2012;S155-S8.
32. Thongson C, Davidson PM, Mahakarnchanakul W, Weiss J. Antimicrobial activity of ultrasound-assisted solvent extracted spices. Lett Appl Microbiol 2004;39:401-6.
33. Almagro L, Gutierrez J, Pedreno MA, Sottomayor M. Synergistic and additive influence of cyclodextrins and methyl jasmonate on the expression of the terpenoid indole alkaloid pathway genes and metabolites in Catharanthus roseus cell cultures. Plant Cell Tissue Organ Cult 2014;119:543–51.
312 Views | 371 Downloads
How to Cite
Yadav, A. K., S. K. Ambasta, S. K. Prasad, and M. P. Trivedi. “IN VITRO EVALUATION OF ANTIBACTERIAL PROPERTY OF CATHARANTHUS ROSEUS (LINN.) G. DON. VAR. “ROSEA” AND “ALBA””. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 5, May 2018, pp. 55-58, doi:10.22159/ijpps.2018v10i5.24977.
Original Article(s)