PHARMACOGNOSTIC STANDARDISATION AND CHROMATOGRAPHIC FINGERPRINTING OF LEAVES AND FRUITS OF ZANTHOXYLUM RHETSA
Keywords:Zanthoxylum rhetsa, Fruit, Leaf, Microscopy, Fingerprint, Standardisation
Objective: To conduct pharmacognostic standardisation and chromatographic fingerprinting of leaves and fruits of Zanthoxylum rhetsa.
Methods: The macroscopic, microscopic and physicochemical evaluation of the crude drugs were conducted as per I. P and WHO guidelines. The chromatographic fingerprint was also developed for the leaves and fruits.
Results: The microscopic characteristics of leaves exhibited anomocytic stomata, fibrovascular tissue, lignfied pericyclic fibers and pitted xylem vessels. The fruits exhibited pericarp, oil cells, stone cells, xylem and endosperm. The physicochemical analysis was also conducted. The leaf and fruit powders complied with WHO prescribed limits for microbial load NMT 1Ã— 105 CFU/ml and were found to be free from pathogenic organisms. The HPTLC fingerprint was established for methanol extract of leaves and fruits using Dichloromethane: chloroform: ethanol (4:4:1) as solvent and 10% methanolic sulphuric acid as spray reagent.
Conclusion: The present work provides referential information for the correct identification and standardisation of the plant.
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