PHARMACOGNOSTIC STANDARDISATION AND CHROMATOGRAPHIC FINGERPRINTING OF LEAVES AND FRUITS OF ZANTHOXYLUM RHETSA


Rashmi Mallya, Milind J. Bhitre

Abstract


Objective: To conduct pharmacognostic standardisation and chromatographic fingerprinting of leaves and fruits of Zanthoxylum rhetsa.

Methods: The macroscopic, microscopic and physicochemical evaluation of the crude drugs were conducted as per I. P and WHO guidelines. The chromatographic fingerprint was also developed for the leaves and fruits.

Results: The microscopic characteristics of leaves exhibited anomocytic stomata, fibrovascular tissue, lignfied pericyclic fibers and pitted xylem vessels. The fruits exhibited pericarp, oil cells, stone cells, xylem and endosperm. The physicochemical analysis was also conducted. The leaf and fruit powders complied with WHO prescribed limits for microbial load NMT 1× 105 CFU/ml and were found to be free from pathogenic organisms. The HPTLC fingerprint was established for methanol extract of leaves and fruits using Dichloromethane: chloroform: ethanol (4:4:1) as solvent and 10% methanolic sulphuric acid as spray reagent.

Conclusion: The present work provides referential information for the correct identification and standardisation of the plant.


Keywords


Zanthoxylum rhetsa, Fruit, Leaf, Microscopy, Fingerprint, Standardisation

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About this article

Title

PHARMACOGNOSTIC STANDARDISATION AND CHROMATOGRAPHIC FINGERPRINTING OF LEAVES AND FRUITS OF ZANTHOXYLUM RHETSA

Keywords

Zanthoxylum rhetsa, Fruit, Leaf, Microscopy, Fingerprint, Standardisation

DOI

10.22159/ijpps.2018v10i4.25089

Date

01-04-2018

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 4, 2018 Page: 101-104

Online ISSN

0975-1491

Authors & Affiliations

Rashmi Mallya
SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, Gate No: 1 Mithibai College Campus, Vile Parle (W), Mumbai 400052, India

Milind J. Bhitre
C.U. Shah College of Pharmacy, SNDT Women’s University, Santacruz (West), Mumbai 400049, India


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