ASSESSMENT OF SECONDARY METABOLITES WITH RELATION TO THEIR ANTIOXIDANT ACTIVITY OF FUNGAL ENDOPHYTES ISOLATED FROM MEDICINAL PLANTS
Keywords:Medicinal plants, Fungal endophytes, Secondary metabolites, Antioxidant, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity
Objective: The detection of fungal endophytes and assessment of their antioxidant activity isolated from two medicinally important plants, Calotropis procera (L.) R. Br. and Catharanthus roseus (L.) G. Don. from parts of Burdwan district of West Bengal, India.
Methods: Isolation of fungal endophytes was carried out following the standard methods in potato dextrose agar (PDA) media. Identification, determination of colonization frequency, fermentation and extraction was done as stated in the standard protocol. Qualitative assay of the bioactive compound has investigated by standard protocol specifically for them. Quantitative estimation of total phenol was done by spectrophotometric method using gallic acid as standard. The antioxidant activity of the cell-free extract was determined by phosphomolybdenum assay and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method.Results: Three dominant endophytic fungi each from C. procera and C. roseus with high colonization frequency in Penicillium singorense 25% and Alternaria alternata 21.87 % respectively has isolated and identified. Qualitative detection of secondary metabolites revealed that Penicillium singorense isolated from both plants and Aspergillus neoflaviceps (from C. procera) were found to be able to produce all the functional metabolites so far tested. It is evident that highest phenol 27.65Â±1.2 mg/g and flavonoid 2.06Â±0.1 mg/g content was shown by Curvularia geniculata. Highest antioxidant activity was exhibited by C. geniculata in both phosphomolybdenum assay i.e., total antioxidant capacity 2.46Â±0.11 and % inhibition of DPPH radical 94.55Â±0.015%.
Conclusion: Present study, therefore highlights the growing concept that the bioactive compounds produced by the endophytes not only established host endophyte relationship but also have an immense chance of an application in the field of medicine, agriculture and industry.
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