GC-MS BASED CHEMICAL PROFILING AND EVALUATION OF ANTIOXIDANT POTENTIAL OF LEAVES AND STEMS OF ALTERNANTHERA SESSILIS RED FROM SABAH, MALAYSIA

  • Mohammad Shaheen Khan Industrial Chemistry Programme, Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia
  • Samina Khan Yusufzai School of Industrial Technology, Universiti Sains Malaysia, 11800 Minden, Pulau Penang, Malaysia
  • Lim Yi Ying Industrial Chemistry Programme, Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia
  • Wan Zulnashriq Industrial Chemistry Programme, Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia

Abstract

Objectives: The aim of the current study was to perform phytochemical screening and determination of total phenolics, total flavonoids and antioxidant activity of various crude extracts of Alternanthera sessilis red leaves.

Methods: Determination of antioxidant nature of Alternanthera sessilis red was carried out by DPPH radical scavenging method. GC-MS analysis of various crude extracts resulted in the presence of different types of low and high molecular weight compounds consisting of carbohydrates, fatty acid and vitamins.

Results: Among all the extracts, methanol extracts exhibited higher DPPH radical scavenging activity with IC50 value of 0.183 mg/ml. BHT used as the positive control showed IC50 value of 0.089 mg/mL. The results suggested that the crudes extracts of Alternanthera sessilis red exhibit moderate antioxidant activity. The total phenolic content (279.8 ± 0.02 mg GAE/g) and total flavonoid content (250.7 ± 0.03 mg QE/g) in methanol was estimated higher as compared to other solvents.

Conclusion: The total flavonoid content and total phenolic content were  found to be higher in methanolic extract followed by ethyl acetate  and hexane extracts.

Keywords: Alternanthera sessilis, Total phenolics, Total flavonoids, DPPH activity, BHT, GC-MS analysis.

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Khan, M. S., S. K. Yusufzai, L. Y. Ying, and W. Zulnashriq. “GC-MS BASED CHEMICAL PROFILING AND EVALUATION OF ANTIOXIDANT POTENTIAL OF LEAVES AND STEMS OF ALTERNANTHERA SESSILIS RED FROM SABAH, MALAYSIA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 7, July 2018, pp. 4-9, doi:10.22159/ijpps.2018v10i7.25204.
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Review Article(s)