DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF CURCUMIN, PIPERINE AND CAMPHOR IN AN AYURVEDIC FORMULATION


Sultana Shaikh, Vandana Jain

Abstract


Objective: To develop a novel, accurate, precise and linear reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous qualitative and quantitative estimation of curcumin, piperine and camphor in an ayurvedic formulation and validate as per international conference on harmonization (ICH) guidelines.

Methods: In the present work, good chromatographic separation was achieved isocratically using a shim-pack HPLC C18 column (4.6 x 250 mm, 5μm) and mobile phase consisting of 0.02 M potassium dihydrogen orthophosphate buffer (pH adjusted to 3.5 with orthophosphoric acid) and acetonitrile in the ratio 40:60, at flow rate of 1 ml/min and column temperature maintained at 35 °C. The effluents obtained were monitored at 255 nm with UV-visible detector.

Results: The retention time of curcumin, piperine and camphor was found to be 6.57 min, 7.32 min and 8.57 min respectively. Linearity of curcumin and camphor were found in the range of 4-8 ppm and that of piperine was found to be 5-9 ppm. The correlation coefficient for curcumin, piperine and camphor were 0.998, 0.99 and 0.994 respectively. The high recovery values (98 %-102 %) indicate a satisfactory accuracy. The low percent relative standard deviation (% RSD) values in the precision study reveals that the method is precise.

Conclusion: The developed method is novel, simple, precise, rapid, accurate and reproducible for simultaneous quantitative estimation of curcumin, piperine and camphor in an ayurvedic formulation. Hence the developed method can be used for quantitative analysis and quality control of extracts and commercial samples of other species containing these three markers.


Keywords


Curcumin, Piperine, Camphor, Ayurvedic formulation, RP-HPLC, Validation, ICH

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About this article

Title

DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF CURCUMIN, PIPERINE AND CAMPHOR IN AN AYURVEDIC FORMULATION

Keywords

Curcumin, Piperine, Camphor, Ayurvedic formulation, RP-HPLC, Validation, ICH

DOI

10.22159/ijpps.2018v10i4.25216

Date

01-04-2018

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 4, 2018 Page: 115-121

Online ISSN

0975-1491

Authors & Affiliations

Sultana Shaikh
M. PHARM Student, Department of Quality Assurance, Oriental College of Pharmacy, Sanpada - Navi Mumbai, Maharashtra 400 705.
India

Vandana Jain
Associate Professor, Department of Quality Assurance, Oriental College of Pharmacy, Sanpada - Navi Mumbai, Maharashtra 400 705.
India


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