• Abhirama B. R. Department of Pharmaceutical Chemistry, J.K.K. Nattraja College of Pharmacy, Dr. MGR medical university, Komarapalayam 638138, Tamilnadu, India.
  • Shanmuga Sundaram R Department of Pharmacology, J.K.K. Nattraja College of Pharmacy, Komarapalayam 638138, Tamilnadu, India. Affiliated to Tamilnadu Dr.M.G.R.Medical University, Chennai 600032, Tamilnadu, India
  • Raju A Department of Pharmacology, St Joseph College of Pharmacy , Cherthala, Kerala. Affiliated to KUHS, Trissur, Kerala.


Objective: Present study was undertaken to investigate the nephroprotective activity of whole plant ethanol extract of Biophytum sensitivum Linn. DC (B. sensitivum; EEBS) on gentamicin induced-nephrotoxicity in Wistar albino rats.

Methods: Animals were divided into five groups, containing six animals in each. Gentamicin (GM) 100 mg/kg/d; i. p., was given to all groups except normal control to induce nephrotoxicity for a period of 8 d in rats. Animals in Group I served as control and Group II as GM-treated or nephrotoxic control. Group III received standard quercetin (50 mg/kg; p. o.); group IV received EEBS (250 mg/kg; p. o.) and group V received EEBS (500 mg/kg; p. o.), for 8 d. Several renal functional tests and injury markers such as a change in body weight, water intake, urine volume and pH, urinary levels of total protein, albumin, sodium, potassium, calcium and magnesium were measured. Different hematological parameters including, red blood cells (RBC), hemoglobin (Hb), packed cell volume (PCV), mean corpuscular haemoglobin (MCH), white blood corpuscles (WBC), lymphocyte, monocyte, polymorphs and eosinophil were also analyzed.

Results: The results revealed that co-administration of EEBS at 250 and 500 mg/kg significantly reduced the urinary excretion of total protein (4.05±0.04, 3.53±0.05 g/dl), albumin (0.81±0.08, 0.71±0.00 g/dl), calcium (8.05±0.05, 7.81±0.06 mg/dl) and magnesium (0.79±0.05, 0.77±0.06 mg/dl) respectively. EEBS treatment in group IV and V animals produced a significant increase in body weight (2.58±0.17, 2.52±0.22 g), water intake (17.35±0.82, 17.87±0.86 ml/24 h) urine output (7.24±0.15, 7.88±0.08 ml/24 h), compared to animals in group II. Co-therapy with both doses of extracts also showed significant increase in RBC (6.85±0.31, 7.10±0.43 million/mm3) Hb (13.96±0.21, 13.83±0.31 g/dl), MCH (20.45±0.49, 20.67±0.51 pg) and PCV (38.29±0.42, 38.53±0.20%) levels, whereas WBC (8.24±0.21, 8.15±0.10 1X103/mm3) and polymorphs (14.19±0.20, 14.05±0.11%) levels were found to be significantly reduced respectively in animals than those in toxic control group.

Conclusion: This study substantiated and confirmed the ethnomedical usefulness of B. sensitivum as a nephroprotective and antioxidant agent.

Keywords: Biophytum sensitivum, Gentamicin, Hematological parameters, Nephroprotection, Quercetin


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1. Ho JL, Barza M. Role of aminoglycoside antibiotics in the treatment of intra-abdominal infection. Antimicrob Agents Chemother 1987;31:485-91.
2. Edson RS, Terrell CL. The aminoglycosides. Mayo Clin Proc 1999;74:519-28.
3. Lerner SA, Schmitt BA, Seligsohn R, Matz GJ. Comparative study of ototoxicity and nephrotoxicity in patients randomly assigned to treatment with amikacin or gentamicin. Am J Med 1986;80:98-104.
4. Abdel-Raheem IT, Abdel-Ghany AA, Mohamed GA. Protective effect of quercetin against gentamicin-induced nephrotoxicity in rats. Biol Pharm Bull 2009;32:61-7.
5. Bhattacharjee S. Reactive oxygen species and oxidative burst: roles in stress, senescence and signal transduction in plants. Curr Sci 2005;89:1113-21.
6. Ozbek E, Ilbey YO, Simsek A, Cekmen M, Mete F, Somay A. Rosiglitazone, peroxisome proliferator receptor-gamma agonist, ameliorates gentamicin-induced nephrotoxicity in rats. Int Urol Nephrol 2010;42:579-87.
7. Shin HS, Yu M, Kim HS, Choi HS, Khang DH. Renoprotective effect of red ginseng in gentamicin-induced acute kidney injury. Lab Invest 2014;94:1147-60.
8. Editorial Committee of the Flora of Taiwan. Flora of Taiwan. 2nd ed. Vol. III. Taipei: Epoch Pub; 1993.
9. Yun LL, Wan YW. Chemical constituents of Biophytum sensitivum. Chin Pharm J 2003;55:71-5.
10. Leopold J, Gerhard B, Mohamed SP, Beena J. Medicinally used plants from India: Analysis of the essential oil of air-dried Biophytum sensitivum (L.) DC. Sci Pharm 2004;72:87-96.
11. Guruvayorappanc C, Afira AH, Girij K. Antioxidant potential of Biophytum sensitivum extract in vitro and in vivo. J Basic Clin Physiol Pharmacol 2008;17:255-67.
12. Natarajan D, Shivakumar MS, Srinivasan R. Antibacterial activity of leaf extract of Biophytum sensitivum (L.) DC. J Sci Res 2010;2:717-20.
13. Puri D. Screening of mildly hypoglycemic compounds: obese British Angora rabbits with borderline glucose intolerance as an animal model. Indian J Pharm Sci 2006;68:579-83.
14. Guruvayoorappan C, Kuttan G. Amento flavone inhibits experimental tumor metastasis through a regulatory mechanism involving MMP-2, MMP-9, prolyl hydroxylase, lysyloxidase,VEGF, ERK-1, ERK-2, STAT-1, nm23 and cytokines in lung tissues of C57BL/6mice. Immunopharmacol Immunotoxicol 2008;30:711-27.
15. Jachak SM, Bucar F, Kartnig T. Anti-inflammatory activity of extracts Biophytum sensitivum in carrageenin-induced rat paw edema. Phytother Res 1999;13:73-4.
16. Puri D. Hypocholesterolemic effect of Biophytum Sensitivum leaf water extract. Pharm Biol 2003;41:253-8.
17. Azhar-Alam MM, Javed K, Jafri MA. Effect of Rheum emodi (Revand Hindi) on renal functions in rats. J Ethnopharmacol 2005;96(1 Suppl 2):121-5.
18. Rajesh KS, Rajani GP, Vivek S, Komala N. Effect of ethanolic and aqueous extracts of Bauhinia VariegataLinn. on gentamicin-induced nephrotoxicity in rats. Indian J Pharm Edu Res 2011;l45:192-8.
19. Kirtikar KR, Basu BD. Indian medicinal plants. 2nd ed. Vol. I. Dehradun: International Book Distributors; 1999. p. 1045-8.
20. Harborne AJ. Phytochemical methods. A guide to modern techniques of plant analysis. 3rd ed. Springer Sciences and Business Media; 1998. p. 334.
21. Anindya B, Md Harun AR, Abdur R, Tapas KP. In vivo screening of ethanolic extract of Biophytum sensitivum DC leaves on peptic ulcer induced by aspirin in wistar albino rats. Int J Pharm Phytopharmacol Res 2014;3:418-22.
22. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin-phenol reagent. J Biol Chem 1951;193:265-75.
23. Reinhold JG. Total protein, albumin and globulin. Stand Methods Clin Chem 1953;1:88-97.
24. Maruna RF, Trinder SR. Determination of serum sodium by colorimetric method. Clin Chim Acta 1957;2;581-5.
25. Maruna RFL. Determination of serum potassium by colorimetric method. Clin Chem Acta 1957;2:131-3.
26. Lorenz K. Improved determination of serum calcium with ortho-cresolphthalein complex one. Clin Chim Acta 1982;126:327-34.
27. Heaton FW. Determination of magnesium by the Titan yellow and ammonium phosphate methods. J Clinl Pathol 1960;13:358-60.
28. Ramnic S. Textbook of medical lab technology. 1st ed. New Delhi: Jypee; 2007. p. 204-21.
29. John MB. Laboratory medicine hematology. 4th ed. ST Louis: CV Mosby Co; 1972. p. 1198-204.
30. Desouza VB, de-Oliveira RFL, de-Lucena HF, Antunes AA, Guerra GCB, Freitas ML, et al. Gentamicin induces renalmorphopathology in Wistar rats. Int J Morphol 2009;27:59-63.
31. Li CC, Finley DS, Uribe C, Eichel L, Lee DI, McDougall EM, et al. Effect of urine specific gravity on the effectiveness of shockwave lithotripsy. J Endourol 2005;19:167.
32. Wolf G, Ziyadeh FN. Cellular and molecular mechanisms of proteinuria in diabetic nephropathy. Nephron Physiol 2007;106:-26-31.
33. Derakhshanfar A, Bidadkosh A, Kazeminia S. Vitamin E protection against gentamicin-induced nephrotoxicity in rats: a biochemical and histopathologic study. Iran J Vet Res 2007;8:231-8.
34. Foster JE, Harpur S, Garland HO. An investigation of the acute effect of gentamicin on the renal handling of electrolytes in the rat. J Pharmacol Exp Ther 1992;261:38-43.
35. Paprika MV, Sharma BB. Protective effect of sesame oil against lead acetate induced hematao-biochemical toxicity in albino rats. J Cell Tissue Res 2003;3:12-7.
36. Patocka J, Cerny K. Inorganic lead toxicology. Acta Medica (Hradec Kralove) 2003;46:65-72.
37. Lavicoli I, Carelli G, Stanek EJ, Castellino N, Calabrese EJ. Effects of low doses of dietary lead on red blood cell production in male and female mice. Toxicol Lett 2003;137:193-9.
38. Ashour AA, Yassin MM, Abu Aasi NM, Ali RM. Blood, serum glucose and renal parameters in lead-loaded albino rats and treatment with some chelating agents and natural oils. Turk J Biol 2007;31:25-34.
39. Sharma R, Panwar K, Mogra S. Alterations in developing RBCs after prenatal and postnatal exposure to lead acetate and Vitamins. Int J Pharm Sci Res 2013;4:3214-24.
40. Adikwu E, Deo O, Geoffrey, OBP, Enimeya DA. Lead organ and tissue toxicity: roles of mitigating agents (Part 1). Br J Pharm Toxicol 2013;4:232-40.
41. Vaziri ND, Liang K, Ding Y. Increased nitric oxide inactivation by reactive oxygen species in lead-induced hypertension. Kidney Int 1999;56:1492-8.
42. Ebtihal ET, Sania AS, Muddathir AK, Muha MEA. Nephroprotective effect of Acacia senegal against gentamycin-induced renal damage in rats. World J Pharm Res 2016;5:294-303.
43. Polat A, Parlakpinar H, Tasdemir S, Colak C, Vardi N, Ucar M, et al. Protective role of aminoguanidine on gentamicin-induced acute renal failure in rats. Acta Histo Chem 2005;108:365-71.
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How to Cite
R., A. B., S. S. R, and R. A. “AMELIORATION OF GENTAMICIN-INDUCED RENAL DAMAGE IN RATS BY ETHANOL EXTRACT OF THE WHOLE PLANT BIOPHYTUM SENSITIVUM (LINN.) DC”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 5, May 2018, pp. 130-5, doi:10.22159/ijpps.2018v10i5.25514.
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