• Mauro L. G. DE-Oliveira Departamento de Química, Universidade Federal de Minas Gerais Belo Horizonte, MG, Brazil
  • Regina A. G. Assenco Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, MG, Brazil
  • GRACIA D. F. SILVA Departamento de Química, Universidade Federal de Minas Gerais Belo Horizonte, MG, Brazil
  • JULIO C. D. LOPES Departamento de Química, Universidade Federal de Minas Gerais Belo Horizonte, MG, Brazil
  • FERNANDO C. SILVA Departamento de Química, Universidade Federal de Minas Gerais Belo Horizonte, MG, Brazil
  • MARIA C. S. LANNA Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, MG, Brazil
  • JOSE CARLOS DE-MAGALHAES Departamento de Química, Biotecnologia e Engenhariade Bioprocessos, Universidade Federal de São João Del-Rei, MG, Brazil
  • Lucienir P. Duarte Departamento de Química, Universidade Federal de Minas Gerais Belo Horizonte, MG, Brazil
  • Sidney A. Vieira Filho Departamento de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil


Objective: The in silico free access web tools PASS online and ChemMapper were used to predict potential biological activities of compounds 1 to 8 isolated from Maytenus gonoclada (Celastraceae). The constituents 4'-O-methylepigalocatequin (6), tingenone (7) and proanthocyanidin A (8), and ethanolic extracts were subjected to in vitro cytotoxicity using VERO cells and anti-Poliovirus assays.

Methods: QSAR and molecular superposition, correlating the average number of pharmacophores were used in the prediction studies. Cellular line VERO ATCC CCL-81 was used to determine anti-Poliovirus effect, observed by colorimetric (MTT) method. The annexing V/propidium iodide assay was used to determine the occurrence of apoptosis in the cytotoxicity assays.

Results: The experimental results found for constituents 6-8 were in accordance with observed data obtained through PASS online and ChemMapper simulation.

Conclusion: Compound 7 showed higher cytotoxic and apoptosis induction properties, and 6 and 8 presented anti-Poliovirus activity.

Keywords: Maytenus gonoclada, VERO cells Cytotoxicity, Anti-Poliovirus, Apoptosis, ChemMapper, PASS online.


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How to Cite
DE-Oliveira, M. L. G., R. A. G. Assenco, G. D. F. SILVA, J. C. D. LOPES, F. C. SILVA, M. C. S. LANNA, J. C. DE-MAGALHAES, L. P. Duarte, and S. A. Vieira Filho. “CYTOTOXICITY, ANTI-POLIOVIRUS ACTIVITY AND IN SILICO BIOLOGICAL EVALUATION OF CONSTITUENTS FROM MAYTENUS GONOCLADA (CELASTRACEAE)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 10, 1, pp. 130-7,
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