INDUSTRIALLY IMPORTANT ENZYMES PRODUCING STREPTOMYCES SPECIES FROM MANGROVE SEDIMENTS
Objective: To explore the systematic diversity of mangrove actinomycetes as well as screening of top valued enzymes
Methods: The target sampling region was selected on Muthupet mangrove forest, Tamilnadu, India. Soil samples were collected at a depth of 15-25 cm from the land to the marine. All isolates were identified by classical approach by means of morphological, physiological and biochemical characterization. In addition, the ability of selective Streptomyces isolates producing important enzymes such as amylase, protease, lipases, esterase and gelatinase were screened for industrial application.
Results: In total, 205 actinomycetes were isolated from mangrove sediments and most of the genus belongs to Streptomycetes. Among, 35 morphologically distinct Streptomyces species were exhibited positive results for amylase (25%), Protease (21%), Lipase (16%), Esterase (17%) and Gelatinase (21%)
Conclusion: The research focused towards exploring marine actinomycetes as a source for enzyme reservoir to compensate current industrial demands.
2. Holguin G, Zamorano PG, De-Bashan LE, Mendoza R, Amador E, Basha Y. Mangrove health in an arid environment encroached by urban development case study. Sci Total Environ 2006;363:260-73.
3. Berdy J. Bioactive microbial metabolites. J Antibiotic (Tokyo) 2005;58:1-26.
4. Fenical W, Jensen PR. Developing a new resource for drug discovery: marine actinomycete bacteria. Nat Chem Biol 2006;2:666-73.
5. Mitra A, Santra SC, Mukherjee J. Distribution of actinomycetes, their antagonistic behavior and the physico chemical characteristics of the worldâ€™s largest tidal mangrove forest. Microbiol Biotechnol 2008;80:685-95.
6. Jagan Mohan YSYV, Sirisha B, Haritha R, Ramana T. Selective screening, isolation and characterization of antimicrobial agents from marine actinomycetes. Int J Pharm Pharm Sci 2013;5(4):443-9.
7. Bull AT, Stach JEM, Ward AC, Goodfellow M. Marine actinobacteria; perspectives, challenges, future directions. Antonie Van Leeuwehoek 2005;87:65-79.
8. Stach JCM, Maldonado LA, Ward AC, Bull AT, Goodfellow M. Williamsia maris sp. nov, a novel actinomycetes isolated from the sea of japan. Int J Syst Evol Microbiol 2004;54:191-4.
9. Oskay M, Tamer A, Azeric P. Antibacterial activity of some actinomycetes isolated from farming soil of turkey. Afr J Biotechol 2004;3(9):441-6.
10. Eccleston GP, Brooks PR, Kurtboke DI. The occurrence of bioactive Micromonospora in aquatic habitats of the sunshine coast in australia. Mar Drugs 2008;6:243-61.
11. Ramesh S, Mathivanan N. Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes. World J Microbial Biotechnol 2009;25:2103-11.
12. Zhang J, Zhang L. Improvement of an isolation medium for actinomycetes. Mod Appl Sci 2011;5:124-7.
13. Cuesta C, Garcia-de-la-Fuente R, Abad M, Fomes J. Isolation and identification of actinomycetes from a compost amended soil with potential as biocontrol agents. J Environ Manage 2012;95:280-4.
14. Enzymes in industrial Applications: Global market. BCC Res; 2012. p. 1.
15. Kumar CG, Takagi H. Microbial alkaline protease from a bio industrial viewpoint. Biotechnol Adv 1999;17:561-94.
16. Uyar F, Baysal Z. Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid-state fermentation. Process Biochem 2004;39:1893-8.
17. Sharma R, Chisti Y, Banerjee UC. Biotechol Adv 2001;19:627-62.
18. Hasan F, Shah AA, Hameed A. Enzyme Microb Technol 2006;39:235-51.
19. Treichel H, Oliveira D, Mazutti MA, Luccio MD, Oliveira JV. Food Bioprocess Technol 2010;3:182-96.
20. Stevenson S, Azavoorian S, Liotta LA. Tumor cell interactions with the extracellular matrix during invasion and metastasis. Annu Rev Cell Biol 1993;9:541-73.
21. Shiriling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:312-40.
22. Nonomura H. Key for and identification of 458 species of the Streptomycetes included in ISP. J Fermet Technol 1974;52(2):78-92.
23. Buchanan RE, Gibbons NE. Bergeyâ€™s manual of determinative bacteriology. (Eighth edition). the Williams and Wilkins Co: Baltimore; 1974;747-842.
24. Henderson G, Krygsman P, Liu CJ, Davey CC, Maluk LT. Characterization ad structure of genes for protease A and B from Streptomyces griseus. Am Soc Microbiol 1987;169:3378-784.
25. Simbert RM Krieg R. Phenotypic characterization: methods for general and Molecular bacteriology. Am Soc Microbiol Washington 1994;607-54.
26. Sierra GA. A simple method for the detection of lipolytic activity of microorganisms and some observations on the influence of the contact between cells and fatty substracts. Anton Lee 1957;28:15-22.
27. Aneja KR. Experiments in Microbiology, Plant pathology and Biotechnology. New Delhi, India: New age international publishers; 2004;4:212-5.
28. Sathya R, Sivagami G, Panneerselvam A. Diversity of Actinomycetes from Muthupet mangrove sediment of Tamiladu, south India. Sci Transactions Environ Technovation 2010;3(3):127-9.
29. Karthikeyan P, Senthilkumar G, Panneerselvam A. Actinobacterial diversity of mangrove environment of Palaverkadu mangroves, east cost of Tamilnadu. Int J Cur Microbiol App Sci 2014;3(1):145-54.
30. Sivakumar K, Sahu MK, Thangarajou T, Kannan L. Research on marine Actinobacteria in India. Indian J Microbiol 2007;47:186-96.
31. Peela S, Rapiraju K, Terli R. Studies on antagonistic marine actinomycetes from the bay of bengal. World J Microbiol Biotechnol 2005;21:583-5.
32. Vijayakumar R, Muthukumar C, Thajuddin N, Panneerselvam A, Saravanamuthu R. Studies on the diversity of actinomycetes in the Palk Strait region of Bay of Bengal, India. Actinomycetologica 2007;21:59-65.
33. Jensen PR, Dwight R, Fenical W. Distribution of actinomycetes in near shore tropical marine sediments. App Env Microbiol 1991;29:1102-8.
34. Hames Kocabas EE Uzel A. Alkaline protease production by actinomycetes MA1-1 isolated from marine sediments. Ann Microbiol 2007;57:71-5.
35. Ramesh S, Rajesh M, Mathivanan N. haracterization of a thermostable alkaline protease produced by marine Streptomyces fugicidicus MML. Bioprocess Biosyst Eng 2009;32:791-800.