PHARMACOGNOSTICAL GENETIC BARCODING AND PHYTOCHEMICAL ANALYSIS ON LEAVES OF DALBERGIA VOLUBILIS ROXB.–AN EXTRA PHARMACOPOEIAL DRUG

  • Acharya Rabinarayan Head of Dravyaguna Department, Room No. 329, 3rd Floor, Institute Building, IPGT andRA, Gujarat Ayurved University, Jamnagar 361008
  • Jani Switu Head of Dravyaguna Department, Room No. 329, 3rd Floor, Institute Building, IPGT andRA, Gujarat Ayurved University, Jamnagar 361008
  • C. R. Harisha Head of Dravyaguna Department, Room No. 329, 3rd Floor, Institute Building, IPGT andRA, Gujarat Ayurved University, Jamnagar 361008
  • Shukla Vinay Head of Dravyaguna Department, Room No. 329, 3rd Floor, Institute Building, IPGT andRA, Gujarat Ayurved University, Jamnagar 361008

Abstract

Objective: The leaves of Biraskala have been reported for its ethnomedicinal claims for the management of aphthae, heart pain, menorrhagia and post-partum problem. Botanically it has been identified as Dalbergia volubilis Roxb. This research article overcomes the lacuna and explores the anatomical, Deoxyribonucleic acid (DNA) barcoding and preliminary phytochemical analysis including High Performance Thin Layer Chromatography (HPTLC).

Methods: After proper authentication, leaves were exposed to macroscopy, microscopy and micrometric analysis and physicochemical, qualitative, quantitative and HPTLC study as per of Ayurvedic Pharmacopoeia of India (API). DNA barcoding of the fresh tender leaves samples were carried out following standard methods.

Results: Macroscopically leaves are compound, unipinnate, imparipinnate, rachis base pulvinus, leaflets 11-13. Microscopy of T. S. of rachis resembles anatomical structure of stem. Schematic outline of T. S. of petiolule is circular in shape with single layer epidermis is followed by ground tissue and centrally located vascular bundle. Schematic outline of T. S. of leaflet is bulged in the center and the extending arms of lamina are linear. Surface study reveals stomatal index to be 33.33. The genetic barcoding confirms the species D. volubilis. Loss on drying was found to be 8.78±0.57. HPTLC results shows 6 peaks and 7 peaks at 254 and 366 nm.

Conclusion: D. volubilis, is a woody climbing unarmed inland shrub with stipules modified into hook and imparipinnately unipinnate compound leaves, are the key field identification character of the plant. Typical microscopical characteristic are presence of solitary crystal mostly embedded in sheath surrounding the vascular bundle and paracyctic type of stomata.

Keywords: Dalbergia volubilis Roxb. DNA barcoding, Pharmacognosy, Anukta dravya

Downloads

Download data is not yet available.

Author Biographies

Acharya Rabinarayan, Head of Dravyaguna Department, Room No. 329, 3rd Floor, Institute Building, IPGT andRA, Gujarat Ayurved University, Jamnagar 361008
Professor and HOD, Department of Dravyaguna, IPGT&RA,
Jani Switu, Head of Dravyaguna Department, Room No. 329, 3rd Floor, Institute Building, IPGT andRA, Gujarat Ayurved University, Jamnagar 361008
SRF, Department of Dravyaguna
C. R. Harisha, Head of Dravyaguna Department, Room No. 329, 3rd Floor, Institute Building, IPGT andRA, Gujarat Ayurved University, Jamnagar 361008
Head, Pharmacignosy laboratory
Shukla Vinay, Head of Dravyaguna Department, Room No. 329, 3rd Floor, Institute Building, IPGT andRA, Gujarat Ayurved University, Jamnagar 361008
Head, Pharmaceuticalchemistry, Laboratory, jamnagar

References

1. Anonyumous an appraisal of tribal folk medicines, CCRAS, New Delhi. 1st edi. 1999. p. 1-2.
2. Khare CP. Indian medicinal plants (an illustrated dictionary). Berlin (Germany): Springer publishers; 2007. p. 201.
3. Koteswara RJ, Seetharami RTVV, Aniel KO. Ethnomedicine of the Gadabas, a primitive tribe of Visskhapatnam District, Andra Pradesh. Internat Multidiscipl Res J 2011:10-4.
4. Brahmam M, Saxena HO. Flora of Orissa. Vol. I. Orissa: Regional Research Laboratory; 1994. p. 522.
5. Gamble JS. Flora of the presidency of Madras. Vol. I. Dehradun: Bishensingh, Manhendra Pal Singh; 2011. p. 522.
6. Neeru V, Manisha V, Sharma SK, Satish S. Chemistry and biological activities of the genus Dalbergia a review. Pharmacogn Rev 2009;3:307-19.
7. Sanjib S, Jamil AS, Himangsu M, Faroque H, Anisuzzman, Mahadhi H, et al. Ethnomedicinal, phytochemical, and pharmacological profile of the genus Dalbergia L.(Fabaceae). Phytopharmacology 2013:291-346.
8. Ida H, Mihaly C, Erik DK, Lene RN, Ida T. The use of DNA barcoding in identification and conservation of rosewood (Dalbergia spp.). Plos One 2015. Doi:10.1371/journal.pone.0138231.
9. Josephin NR, Venkatarathnakumar T, Ajitha DA, Gowri R, Parameshwari R, Shanthi M, et al. Pharmacognostic and preliminary phytochemical evaluation of the leaves of Dalbergia sissoo roxb. Asian J Pharm Clin Res 2012(5, Suppl 3):115-9.
10. Pankaj SN, Dharmendra S, Kiran P, Jain SK. Antidiabetic activity of ethanolic extract of Dalbergia sissoo L. leaves in alloxan induced diabetic rats. Int J Chem Pharm Res 2010;2:24-7.
11. Trease and Evans. Pharmacognosy. 16th ed. London: Saunders Elsevier; 2009. p. 567-68.
12. Anonymous. The Ayurvedic Pharmacopoeia of India. Part-II. Vol-II. 1st ed. New Delhi: Government of India, Ministry of Health and Family Welfare, Department of AYUSH; 2008.
13. Khandelwal KR. Practical pharmacognosy. 19th ed. Pune: Nirali Prakashan; 2008. p. 149.
14. Pankaj BN, Nayan RB, Vinay JS, Rabinarayan A, Mukesh BN. In vitro evaluation of antioxidant activity of Cordia dichotoma (Forst f.) bark. AYU; 2013. p. 124-8.
15. Egon S. Thin layer chromatography. Berlin (Germany): Springer Publication; 1969.
16. Metcalfe CR, Chalk L. Anatomy of the Dicotyledons. Vol. I. Great Britain: Oxford university Press. 1950. p. 502-10.
Statistics
244 Views | 738 Downloads
Citatons
How to Cite
Rabinarayan, A., J. Switu, C. R. Harisha, and S. Vinay. “PHARMACOGNOSTICAL GENETIC BARCODING AND PHYTOCHEMICAL ANALYSIS ON LEAVES OF DALBERGIA VOLUBILIS ROXB.–AN EXTRA PHARMACOPOEIAL DRUG”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 6, June 2018, pp. 117-24, doi:10.22159/ijpps.2018v10i6.26074.
Section
Original Article(s)