PHARMACOGNOSTICAL GENETIC BARCODING AND PHYTOCHEMICAL ANALYSIS ON LEAVES OF DALBERGIA VOLUBILIS ROXB.â€“AN EXTRA PHARMACOPOEIAL DRUG
Objective: The leaves of Biraskala have been reported for its ethnomedicinal claims for the management of aphthae, heart pain, menorrhagia and post-partum problem. Botanically it has been identified as Dalbergia volubilis Roxb. This research article overcomes the lacuna and explores the anatomical, Deoxyribonucleic acid (DNA) barcoding and preliminary phytochemical analysis including High Performance Thin Layer Chromatography (HPTLC).
Methods: After proper authentication, leaves were exposed to macroscopy, microscopy and micrometric analysis and physicochemical, qualitative, quantitative and HPTLC study as per of Ayurvedic Pharmacopoeia of India (API). DNA barcoding of the fresh tender leaves samples were carried out following standard methods.
Results: Macroscopically leaves are compound, unipinnate, imparipinnate, rachis base pulvinus, leaflets 11-13. Microscopy of T. S. of rachis resembles anatomical structure of stem. Schematic outline of T. S. of petiolule is circular in shape with single layer epidermis is followed by ground tissue and centrally located vascular bundle. Schematic outline of T. S. of leaflet is bulged in the center and the extending arms of lamina are linear. Surface study reveals stomatal index to be 33.33. The genetic barcoding confirms the species D. volubilis. Loss on drying was found to be 8.78Â±0.57. HPTLC results shows 6 peaks and 7 peaks at 254 and 366 nm.
Conclusion: D. volubilis, is a woody climbing unarmed inland shrub with stipules modified into hook and imparipinnately unipinnate compound leaves, are the key field identification character of the plant. Typical microscopical characteristic are presence of solitary crystal mostly embedded in sheath surrounding the vascular bundle and paracyctic type of stomata.
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