• Samira Hsaine Department of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco
  • Fatima Zahrae Fethi Dental Office of the Center for Chronic Diseases, Sale, Morocco
  • Reda Charof Department of Medical Bacteriology, National Institute of Hygiene, Rabat, Morocco
  • Khadija Ounine Department of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco,


Objective: Given the importance of the association between diabetes and periodontal disease, the main objective of the present study was to compare the microbial diversity responsible for gingivitis in patients with and without type 2 diabetes.

Methods: Samples were collected from the oral cavity of 134 patients with gingivitis and categorised into 3 groups (68 non-diabetic patients and 66 diabetic patients; 33 with controlled diabetes and 33 with poorly controlled diabetes). Sample culture was carried out on selective culture media. The identification of isolated strains involved a series of biochemical tests including miniature galleries (API 20E and 20 Strep), the traditional biochemical gallery (tubes) and automated bacterial identification (BD Phoenixâ„¢).

Results: Identification by biochemical methods made it possible to differentiate 14 bacterial species and one yeast. There was greater bacterial diversity in diabetic patients as compared to non-diabetic patients. Periodontal pathogens were isolated from both diabetic and non-diabetic populations; however, certain microbes such as Streptococcus acidominimus, Enterobacter cloacae, Klebsiella oxytoca, and Pseudomonas aeruginosa were present only in diabetics, with a much higher percentage in those with poorly controlled diabetes.

Conclusion: Poorly controlled diabetes causes metabolic dysregulation that can increase the severity of periodontal disease.

Keywords: Diabetes, Periodontal disease, Glycemic balance, Microbial diversity


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Author Biographies

Samira Hsaine, Department of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco
département Biology,  PhD Student
Fatima Zahrae Fethi, Dental Office of the Center for Chronic Diseases, Sale, Morocco
doctor -dentist
Reda Charof, Department of Medical Bacteriology, National Institute of Hygiene, Rabat, Morocco

département de Bactériologie Médicale.

Doctor- Biologiste

Khadija Ounine, Department of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco,

Department of Biology, Health and Environment, Applied Microbiology Team, 



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How to Cite
Hsaine, S., F. Z. Fethi, R. Charof, and K. Ounine. “MICROBIOLOGICAL STUDY OF ORAL FLORA IN DIABETIC PATIENTS WITH GINGIVITIS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 6, June 2018, pp. 113-6, doi:10.22159/ijpps.2018v10i6.26295.
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