PHYSICOCHEMICAL, PHYTOCHEMICALS AND ANTIOXIDANT EVALUATION OF GUAZUMA ULMIFOLIA FRUIT
Objective: The present study was designed to evaluate the physicochemical, qualitative, quantitative analysis and antioxidant activity of ethanol fruit extract of Guazuma ulmifolia. Studies were carried out to detect the bioactive component.
Methods: The physiochemical properties such as loss on drying, extractive value for ethyl acetate, acetone, ethanol, total ash, acid insoluble ash, water-soluble ash and PH values; the phytochemical such as tannins, phenol, terpenoids, flavonoids, saponin, quinones, cardiac glycosides, coumarin, steroids and acids were found using standard methods. The total phenol and flavonoid content were estimated by Folinciocalteu and Aluminium chloride by colorimetric method. In vitro antioxidants, properties were evaluated by DPPH and FRAP assay.
Results: Phytochemical analysis reveals the availability of secondary metabolites like tannins, phenol, terpenoids, flavonoids, coumarin, steroids, and cardiac glycosides in the different extract. The quantitative analysis for ethanolic fruit extract was done for total phenolic content (TPC), total flavonoids content (TFC). An ethanolic extract found to have high TPC [27.2797Â±0.1756] than TFC. Antioxidant activity was evaluated by DPPH assay (2, 2-diphenyl-1-picrylhydrazyl-hydrate) and FRAP (ferric Reducing Ability of Plasma).
Conclusion: On the basis of this study, it was found that the Guazuma ulmifolia fruits have potential to act as a source of useful drugs due to the presence of various active phytochemical constituent, which will be helpful in preventing various diseases caused by the oxidative stress.
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