A COMPARATIVE STUDY ON THE IN-VITRO ANTIMICROBIAL ACTIVITY OF THE ROOTS OF FOUR THOTTEA SPECIES

  • Anilkumar Es JNTBGRI, TRIVANDRUM
  • Mathew Dan Jawaharlal Nehru Tropical Botanic Garden and Research Institute
  • Nishanth Kumar S Interdisciplinary Science and Technology
  • Dileep Kumar B S Interdisciplinary Science and Technology
  • Latha P G Jawaharlal Nehru Tropical Botanic Garden and Research Institute

Abstract

Objective: The main objective of the present study was to investigate the antimicrobial activity of the methanol extract of the roots of four Thottea species.

Methods: The root extracts of four Thottea species were subjected to antimicrobial assay by Minimum Inhibitory Concentration (MIC) and Agar Disc diffusion Assay against various medically important pathogens.

Results: It is evident from the study that. Significant antibacterial activity was recorded by Thottea sivarajanii and highest activity was recorded against Pseudomonas aeruginosa and Staphylococcus epidermis (64 µg/ml). Out of the four extracts tested for antifungal activity, Thottea barberi and Thottea ponmudiana recorded significant antifungal activity and the highest activity was recorded by T. barberi against Trichophyton rubrum (16 µg/ml).

Conclusion: Results offer a scientific basis for the traditional use of Thottea species in the treatment of microbial infections, showing that the plant extract has an enormous potential as a prospective alternative drug against microbial pathogens. The present study lays the basis for future studies, to validate the possible use of Thottea species as a candidate in the treatment of microbial infections.

 

Keywords: Antimicrobial activity, Thottea species, MIC, Methanol extract.

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References

1. Okeke IN, Laxminarayan R, Bhutta ZA, Duse AG, Jenkins P, O’Brien TF, et al. Antimicrobial resistance in developing countries. part i: recent trends and current status. Lancet Infect Dis 2005;5:481–93.
2. Iwu MW, Duncan AR, Okunji CO. New antimicrobials of plant origin. In: Janick J. Editor. Perspectives on new crops and new uses. JASHS Press: Alexandria, Va, USA; 1999. p. 457–62.
3. Prabhakar T, Kumar P, Bhogavalli, Narasimha RVB, Reddy S, Gangula, et al. In vitro studies on antimicrobial screening of leaf extracts of Sapindus saponaria against common dental pathogens. Plant Sci Feed 2012;2(2):15–8.
4. Alli AI, Ehinmidu JO, Ibrahim YKE. Preliminary phytochemical screening and antimicrobial activities of some medicinal plants used in ebiraland. Bayero J Pure Appl Sci 2011;4(1):10–6.
5. Trivedi PC. Ethnobotany. Aavishkar Publishers and Distributors, Jaipur, India; 2002. p. 1-12.
6. Bagchi AK. Alternative medicine-old wine in a new bottle. J Indian Med Assoc 2000;98:332–3.
7. Mishra K, Mishra A, Bhargava A, Pandey AK. Antimicrobial activity of essential oils from the leaves of Cinnamomum spp. Natl Acad Sci Lett 2008;31:341–5.
8. Maurya A, Chauhan P, Mishra A, Pandey AK. Surface functionalization of TiO2 with plant extracts and their combined antimicrobial activities against E. faecalis and E. coli. J Res Updates Polym Sci 2012;1:43–51.
9. Neinhuis C, Wake S, Hilu KW, Muller K, Borsch T. Phylogeny of Aristolochiaceae based on parsimony, likelihood and Bayesian analysis of trnL-trnF sequences. Plant Syst Evol 2005;250:7-26.
10. Hou D. Aristolochiaceae. In: Van CGGJ, De Wilde WJJO, editors. Flora of Malesiana. Kulwar Academic Publishers, London; 1984;1(10):53-108.
11. Mabberly DJ. Mabberly’s Plant Book: A portable dictionary of plants, their classification and uses. 3rd ed. Cambridge University, Cambridge; 2008.
12. Karthikeyan S, Sanjappa M, Moorthy S. Flowering plants of India, dicotyledons (Acanthaceae–Avicenniaceae). Botanical Survey of India, Kolkata; 2009. p. 156-7.
13. Nayar TS, Beegam AR, Mohanan N, Rajkumar G. Flowering plants of Kerala –A handbook. Tropical Botanic Garden and Research Institute, Palode. Thiruvananthapuram; 2006. p. 81.
14. Nayar TS, Beegam AR, Mohanan N, Rajkumar G. Flowering plants of Western Ghats of India – dicots. Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode. Thiruvananthapuram; 2014. p. 113,
15. Kirtikar KR, Basu BD. Indian medicinal plants. Reprint ed. Bishen Singh Mahendra Pal Singh, Dehra Dun; 2003. p. 2118.
16. Nair GM, Rajasekharan S, George V. Final scientific and technical report on pilot participatory programme on conservation and sustainable use of medicinal and aromatic plants. Tropical Botanic Garden and Research Institute, Thiruvananthapuram; 2004. Appendix VII. p. 6, 17.
17. Anilkumar N, Sivadasan M, Ravi N. Flora of Pathanamthitta district (Western Ghats, Kerala, India). Daya Publishing House, New Delhi; 2005. p. 415-6.
18. Binu S. An enumeration of medicinal plants used by the tribals in Pathanamthitta district of Kerala, India–part –VI. J Econ Taxon Bot 2013;37(2):274-9.
19. Anonymous. The Wealth of India–Raw Materials. Reprint ed. National Institute of Science Communication and Information Resources, CSIR, New Delhi; 2010. p. 318.
20. Burkill IH. A dictionary of the economic products of the Malay Peninsula. Revised ed. Ministry of Agriculture and Co-operatives, Kuala Lumpur, Malaysia; 1966. p. 2156-7.
21. Radhakrishnan K, Pandurangan AG, Pushpangadan P, Sasidharan A. Less known ethnomedicinal plants of kerala state and their conservation. Ethnobotany 1996;8:82-4.
22. Nazarudeen A, Seeni S, Koshy KC, Pushpangadan P. Folk plants of food, medicine, adornment and repellent used by the Paniyar community in North Kerala. In: Maheswari JK, editor. Ethnobotany of South Asia. Scientific Publishers, Jodhpur, India; 1996. p. 295-305.
23. Nair GM, Rajasekharan S, George V. Final scientific and technical report on pilot participatory programme on conservation and sustainable use of medicinal and aromatic plants. Tropical Botanic Garden and Research Institute, Thiruvananthapuram; 2004. p. 7.
24. Augustine J, Sivadasan M. Ethnomedicinal plants of periyar tiger reserve, kerala, india. Ethnobot 2004;16:44-9.
25. Ajesh TP. Ethnogynaecological studies of the tribes in Idukki district, Kerala, India. Ph. D Thesis, Gandhigram Deemed University, Tami Nadu; 2014.
26. Yoganarasimhan SN, Subramaniyan K, Razi BA. Flora of Chikmagalur district, Karnataka, India. International Book distributors, Dehradun; 1981. p. 273.
27. Subramanian KN. Flora of Thenmala. International Book Distributors, Dehradun; 1995. p. 302.
28. Subbaiah V. Natural product based apoptosis inducers. Phyto Myco Research Corporation, Greenville, NC, US. [Internet]; 2005.
29. CLSI (Clinical and Laboratory Standards Institute). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. CLSI documents M27-S3. 940, West Valley Road, Suite 1400, Wayne, Pennsylvania, 19087-1898 USA; 2006.
30. CLSI (Clinical and Laboratory Standards Institute). Performance standards for antimicrobial disk susceptibility tests; approved standard-11th ed. CLSI documents M02-A11. West Valley Road, Suite 2500, Wayne, PA 19087, USA; 2012.
31. Rollas S, Kalyoncuoglu N, Sur-Altiner D, Yegenglu Y. 5-(4-Aminophenyl)-4-substituted 2,4-dihydro-3H-1,2,4-triazole-3-thiones: synthesis, antibacterial and antifungal activities. Pharmazie 1993;48:308-9.
32. Mishra A, Kumar S, Bhargava A, Sharma B, Pandey AK. Studies on in vitro antioxidant and antistaphylococcal activities of some important medicinal plants. Cell Mol Biol 2011;57:16–25.
33. Wink M. Evolution of toxins and antinutritional factors in plants with special emphasis on Leguminosae. In: Acamovic T, Stewart CS, Pennycott TW, editors. Poisonous plants and related toxins. CABI Publishing, Wallingford, UK; 2004. p. 1–25.
34. Mishra AK, Singh BK, Pandey AK. In vitro antibacterial activity and phytochemicals of (Tejpat) leaf extracts and oil. Rev Infect 2010;1:134–9.
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Es, A., M. Dan, N. Kumar S, D. K. B S, and L. P G. “A COMPARATIVE STUDY ON THE IN-VITRO ANTIMICROBIAL ACTIVITY OF THE ROOTS OF FOUR THOTTEA SPECIES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 10, 1, pp. 444-7, https://innovareacademics.in/journals/index.php/ijpps/article/view/2700.
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