EFFECTS OF STREPTOZOTOCIN INDUCED DIABETES MELLITUS TYPE 1 ON THE RAT BRAIN ANTIOXIDANT STATUS AND ACTIVITY OF ACETYL-CHOLINESTERASE: A NOVEL AND POTENTIAL TREATMENT BY VITEX NEGUNDO.
Objective: The objective of the present study was to determine the effect of streptozotocin (STZ) induced diabetes type 1 on activity of acetyl-cholinesterase (AChE) in rat brain and on the brain antioxidant status and also that whether supplementation with the aqueous extract of Vitex negundo (AEVN) ameliorates neural degeneration caused by hyperglycemia induced oxidative stress in experimental diabetes.
Methods: Male albino rats weighing 180-200g were made diabetic by the single administration of STZ (45 mg/kg body weight) intraperitoneally. AEVN was administered orally through feeding cannula at a dose of 150 mg/kg body weight daily to STZ-induced diabetic rats for 60 days. On 61st day the rats were sacrificed by cervical dislocation and the effects of the AEVN on fasting blood glucose level. AChE activity and on levels of various oxidant and antioxidant enzyme activity in the brain were appraised.
Results: In the result it is observed that STZ-diabetes caused significant elevation in fasting blood glucose, AChE activity and lipid peroxidase (LPO) level. Whereas activity level of the protective antioxidant enzyme, catalase (CAT), reduced glutathione (GSH) and superoxide dismutase (SOD) exhibited significant decline in STZ-diabetes. Supplementation with AEVN attests significant anti-diabetic and antioxidant potential of it as a prominent decrease in fasting blood glucose level and AChE activity was observed. Similarly, the levels of the protective antioxidant enzymes like SOD, CAT and GSH were increased along with the decrease in the level of oxidant enzyme LPO was observed.
Conclusion: The study emphasizes the involvement of diabetes with neural degeneration and point towards the potential beneficial role of AEVN as an adjuvant therapy to conventional anti-hyperglycemic regimens for the prevention and treatment of diabetic encephalopathy.
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