• Martial S. Nounagnon Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin,
  • Durand Dah-nouvlessounon Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin,
  • Christine NTCHA Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin,
  • Boris Legba Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin,
  • Farid Baba-moussa Laboratoire de Microbiologie et de Technologies Alimentaires, Faculté des Sciences et Techniques, Université Abomey-Calavi, 04 BP: 1107 Cotonou, Benin,
  • Adolphe Adjanohoun Centre de Recherche Agricole Sud. Institut National de Recherche Agricole du Benin. Attogon. BP: 884 Cotonou Benin
  • Lamine Baba-moussa Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin,


Objective: This study aims to search the secondary metabolites of Crateva adansonii (leaves and bark) and evaluate some biological activities (antimicrobial, antioxidant and cytotoxicity's property) of these extracts.

Methods: The phytochemical screening was made with standard method. Agar diffusion method was used for antibacterial activity coupled with Minimum Inhibitory Concentration (MIC) and Bactericidal (MBC) determination. The antifungal test was performed by the mycelial development reduction method. DPPH method was used to evaluate the extracts antioxidant activity. Artemia salina larvae were use as support to evaluate the toxicity of the extracts.

Results: The results of the phytochemical screening reveal the presence of polyphenols, triterpenoids, and glycosides in the both organs (leaves and bark) of C. adansonii. All the extracts have low activity on the reference strains with a larger diameter of 17±00 mm (S. oralis). Only the ethanolic and hydroethanolic leaf's extracts inhibited respectively 20% and 10%, of the clinical strain S. aureus. The ethyl acetate leaf's extract shows the best antifungal activity (89,19%) with A. clavatus. All extracts present dose-dependent antiradical properties and are non-toxic for the cells of Artemia salina. C. adansonii bark extracts had the best reducing power (23,80±0, 137 mmol/g EqAA EAA) of the DPPH radical.

Conclusion: C. adansonii is therefore an active principle source for the development of drugs to antimicrobial and antioxidant activities

Keywords: Screening phytochemical, Antimicrobial activity, Anti-radical activity, Benin


Download data is not yet available.


1. Konan F, Guessennd N, Oussou KR, Bahi CA, Coulibaly, Djaman AJ, et al., Effet antibactérien de l’extrait aqueux de l’écorce de Terminalia glaucescens planch ex benth (Combretaceae) sur la croissance in vitro des enterobacteries productrices de beta-lactamases a spectre elargi (EBLSE). Int J Biol Chem Sci 2014; 8:1192-201.
2. Walsh C. Antibiotics: Actions, Origins, Resistance. ASM Press: Washington, DC; 2003.
3. Zohra M. Etude phytochimique et activités biologiques de quelques plantes médicinales de la région nord et sud-ouest de l’algérie. These de doctorat. Universite Abou Bekr Belkaïd; 2013.
4. Dah Nouvlessounon D, Adoukonou Sagbadja H, Diarrassouba N, Sina H, Adjonohoun A, Inoussa M, et al. Phytochemical analysis and biological activities of Cola nitida bark. Biochem Res Int 2015:1-12.
5. Ahama K, Quashie MA, Agbonon I, Koumaglo K. Activites antioxydantes in vitro des feuilles de Crataeva adansonii. Rev Ivoir Sci Technol 2010;16:153-64.
6. Devasagayam TPA, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Phys India 2004;52:794-804.
7. Sofowora A. Medicinal plants and traditional medicine in Africa, Spectrum Books Limited, Sunshine House, 2nd edition. Ibadan, Nigeria; 1993.
8. WHO. Strategie de l'OMS pour la medicine traditionnelle pour 2002–2005; 2002.
9. Fankam AG, Kuiate JR, Kuete V. Antibacterial activities of Beilschmiedia obscura and six other Cameroonian medicinal plants against multi-drug resistant gram-negative phenotypes. BMC Complement Altern Med 2014;14:241-50.
10. Adomou AC, Yedomonhan H, Conhan A, Djossa B, Legba SI, Oumorou M, et al., Etude ethnobotanique des plantes médicinales vendues dans le marche d’Abomey-Calavi au Benin. Int J Biol Chem Sci 2012;6:745-72.
11. Houghton DJP, Raman A. Laboratory handbook for the fractionation of natural extracts. Eur J Publ 1998;71:5-102.
12. Sanogo R, Diallo D, Diarra S, Ekoumou C, Bougoudogo D. Activite antibacterienne et antalgique de deux recettes traditionnelles utilisees dans le traitement des infections urinaires et la cystite au Mali. Mali Med 2006;21:18-24.
13. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.
14. Dohou N, Yamni K, Badoc A, Douira A. Activity antifongique d’extraits de Thymelaea lythroides sur Trois champignons pathogènes du riz. Bull Soc Pharm 2004;143:31-8.
15. Kim HP, Son KH, Chang HW, Kong SS. Anti-inflammatory plant flavonoids and cellular action macanism. J Pharm Sci 2004;96:229-54.
16. Singleton VL, Orthofer R, Lamuela Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteau reagent. Meth Enzym 1999; 299:152-78.
17. Farshori NN, Al-Oqail MM, Al-Sheddi ES, Siddiqui MA, Rauf A. Antimicrobial potentiality of polyalthia longifolia seed oil against multidrug-resistant (MDR) strains of bacteria and fungus of clinical origin. Afr J Microbiol Res 2013;19:1977-82.
18. Agbankpe AJ, Dougnon TV, Bankole SH, Houngbegnon O, Dah-Nouvlessounon D, Baba Moussa L. In vitro antibacterial effects of Crateva adansonii, vernonia amygdalins and Sesamum radiatum used for the treatment of infections diarrheas in Benin. Infect Dis Ther 2016;4:1-7.
19. Tsado AN, Bashir L, Saba E, Saba AM, Balarabe H, Abdusalam, et al. Phytochemicals and acute toxicity profile of aqueous and methanolic extracts of Crateva adansonii leaves in swiss albino rats. J Biochem 2015;10:173-9.
20. Eldridge W, Swain W, Kwolek WF. Northen regional research center agricultural research service. U. S. Department of Agriculture, Peoria, IL 61604: and W. R. FEHR. Department of agronomy, lowa State University Ames, IA50011; 1983.
21. Salminen S, Gibson GR, Mc Cartney AL, Isolauri E. Influence of mode of delivery on gut microbiota composition in seven-year-old children. Gut 2004;53:1388-9.
22. Bruneton J. Phytochimie, plantes médicinales Les tanins. 3th ed. Editions TEC. Et DOC. Editions medicales internationales, Paris; 1999.
23. Lagnika L, Anagonou E, Atindehou M, Adjahoutonon B, Dramane K, Sanni A. Antimicrobial activity of Crateva religiosa Forst against bacteria isolated from thryonomys swinderianus temminck. Afr J Biotechnol 2011;10:10034-9.
24. Nounagnon SM, N’tcha C, Sina H, Noumavo AP, Dah-Nouvlessounon D, Assogba MF, et al. Antimicrobial activities of Combretum micranthum extracts on Staphylococcus aureus strains isolated from skin infections and some reference strains. Asian J Plant Sci Res 2016;6:40-7.
25. Adeyemi OO, Akindele AJ, Yemitan G, Aigbe FR, Fagbo FI. Anticonvulsant, anxiolytic and sedative activities of the aqueous root extract of Securidaca longepedunculata Fresen. J Ethnopharm 2010;20:191-5.
26. Reyes GS, Brown J, Chapman J, Lugo AE. Wood densities of tropical tree species general technical report SO-88. USDA forest service, Southern Forest experiment Station, New Orléans, Louisiana, USA; 1992.
27. Rohman A, Riyanto S, Yuniarti N, Saputra WR, Utami R, Mulatsih W. Antioxidant activity, total phenolic, and total flavonoid of extracts and fractions of red fruit (Pandanus conoideus Lam). Int J Food Res 2010;17:97-106.
28. Kplolali YS, Ahama ML, Akossiwoa Q, Agbonon I, Koumaglo K. Activités antioxydantes in vitro des feuilles de crateva adansonii. Rev Ivoir Sci Technol 2010;16:153-64.
29. Moshi MJ, Cosam JC, Mbwambo ZH, Kapingu M, Nkunya MHH. Testing beyond ethnomedical claims: brine shrimp lethality of some tanzanian plants. Pharm Biol 2004;42:547-51.
30. Pelka M, Danzl C, Distler W, Petschelt A. A new screening test toxicity testing of dental materials. J Dent 2000;28:341-5.
31. Mc Lauglin JL, Chang CJ, Smith DL. Simple bench-top bioassays (brine shrimp and potato discs) for the discovery of plant antitumor compounds. In: Human Medicinal Agents from Plants. Kinghorn AD, Balandrin MF. Eds. American Chemical Society, Washington, DC; 1993.
32. Carballo JL, Hernandez-Inda ZL, Perez P, Garcia-Gràvalos MD. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotech 2002; 2:17-21.
33. Lagarto Parra A, Yhebra RS, Sardinas RIG, Buela LI. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine the acute oral toxicity of plant extracts. Phytomed 2001;8:395-400.
469 Views | 323 Downloads
How to Cite
Nounagnon, M. S., D. Dah-nouvlessounon, C. NTCHA, B. Legba, F. Baba-moussa, A. Adjanohoun, and L. Baba-moussa. “PHYTOCHEMISTRY AND BIOLOGICAL ACTIVITIES OF CRATEVA ADANSONII EXTRACTS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 9, Sept. 2018, pp. 62-67, doi:10.22159/ijpps.2018v10i9.27197.
Original Article(s)