EFFECT OF ETHANOL STEM BARK EXTRACT OF BERLINIA GRANDIFLORA HUTCH AND DALZ ON MARKER LIVER ENZYMES IN RATS TREATED WITH CCl4
Objective: The objective of this work was to validate the traditional claim of the use of the stem bark of Berlinia grandiflora in treating liver problems by investigating the effect of its ethanol stem bark extract on maker liver enzymes in vivo.
Methods: Groups of Wistar albino rats (6 in each group) were daily treated with CCl4 (2 ml/kg b.w., diluted with olive oil 1:1 v/v, intravenously), followed by oral administration of ethanol stem bark extract of Berlinia grandiflora (ESBG) at doses 100, 300 and 900 mg/kg b.w. respectively, 3 h after the administration of the CCl4. Serum biochemical parameters were measured 24 h at the end of the 7-day treatment period and compared to a group intoxicated with CCl4 (2 ml/kg b.w., diluted with olive oil 1:1 v/v, intravenously) alone.
Results: Treatment of rats with ESBG (at doses 100, 300 and 900 mg/kg b.w. orally) reduced the impact of CCl4-induced hepatotoxicity on liver maker enzymes Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP) and total bilirubin (TB), with the 900 mg/kg b.w. showing a significant reduction (p<0.05) compared to the group intoxicated with CCl4. The extract reversed the impact of the CCl4-induced hepatotoxicity by increasing the concentration of the serum Total protein (TP) and albumin (ALB), with the 900 mg/kg b.w. dose showing a significant increment (p<0.05) compared to the group intoxicated with the CCl4.
Conclusion: The investigation of the effect of Berlinia grandiflora on CCl4 induced liver damage revealed that the ethanol extract of the stem bark of the plant was able to reverse the hepatotoxicity, with the 900 mg/kg b.w. dose showing a significant activity.Â
2. Balne D, Pallerla P, Vanapatla S, Bobbala RK. Hepatoprotective effect of whole plant extract fractions of Marsilea minuta Linn. Asian J Pharm Clin Res 2013;6:100-7.
3. Parmar SR, Vashrambhai PH, Kalia K. Hepatoprotective activity of some plants extract against paracetamol-induced hepatotoxicity in rats. J Herbal Med Toxicol 2010;4:101-6.
4. Mahmood ND, Mamat SS, Kamisan FH, Yahya F, Kamarolzaman MFF, Nasir N, et al. Amelioration of paracetamol-induced hepatotoxicity in rat by the administration of methanol extract of Muntingia calabura L. leaves. BioMed Res Int 2014. http://dx.doi.org/10.1155/2014/695678
5. WHO. WHO traditional medicine strategy 2014-2023. Geneva: World Health Organisation; 2013.
6. Kumar A, Pai MO, Rai N. In vitro hepatoprotective activity of Albizia lebbeck, Cassia occidentalis and Swertia chirata on HEPG2 cells. Asian J Pharm Clin Res 2016;9:276-80.
7. Pradeepa M, Kalidas V, Geetha N. Qualitative and quantitative phytochemical analysis and bactericidal activity of Pelargonium graveolens Lâ€™her. Int J Appl Pharm 2016;8:7-11.
8. Duru CE, Duru IA, Ikpa CBC, Ibe FC. Chemical and spectra studies of the alleged killer seed of Berlinia grandiflora. IOSR J Appl Chem 2014;7:14-8.
9. Josephs GC, Ching FP, Nnabuife AC. Investigation of the antimicrobial potentials of some phytochemical extracts of leaf and stem bark of Berlinia grandiflora (Leguminoceae) Caesalpinioidae) against pathogenic bacteria. Afr J Pharmacol Ther 2012;1:92-6.
10. Ode O, Nwaehujor C, Nwinyi C. Evaluation of the anti-diabetic and antioxidant activities of the methanol leaf extract of Berlinia grandiflora. J Med Sci 2013;13:743-8.
11. Enwerem NM, Wambebe CO, Okogun JI, Akah PA, Gamaniel KS. Anthelmintic screening of the stem bark of Berlina grandiflora. J Nat Rem 2001;1:17-20.
12. Asuzu IU, Nwele OC, Anaga AO. The pharmacological activities of the bark methanolic extract of Berlinia grandiflora. Fitoterapia 1993;64:529-34.
13. Bhowal M, Das L. Phytochemical analysis of a fodder crop Trifolium alexandrium L. Int J Pharm Phytochem Res 2014;6:379-82.
14. Harborne J. Phytochemical methods: a guide to modern techniques of plant analysis. 3rd ed. New York: Chapman and Hall; 1998.
15. Sofowora A. Medicinal plants and traditional medicine in Africa. Ibadan: Spectrum Books Ltd; 1993.
16. OECD. OECD guideline for testing of chemicals. Paris: Organization for Economic Cooperation and Development; 2001.
17. Firdous S, Raju K, Pallab H, Gopalakrishnan B. Evaluation of hepatoprotective activity of saponin of Momordica dioica Roxb. against carbon tetrachloride induced hepatic injury in rats. Pharmacologyonline 2008;3:487-94.
18. Hassan SW, Tillo MK, Lawal M, Umar RA, Ndakotsu MA, Farouk U, et al. Hepatoprotective action of stem bark extracts of Newbouldia laevis in rats treated with carbon tetrachloride (CCl4). J Global Biosci 2015;4:1627-46.
19. Goudar M, Jayadevappa H, Mahadevan KM, Shastry RA, Habbu PV, Sayeswara HA. Isolation and characterization of a secondary metabolite from Habenaria intermedia D. Don for screening of hepatoprotective potential against carbon tetrachloride induced toxicity in albino rat liver. Int J Curr Pharm Res 2015;7:57-61.
20. Boll M, Webber LWD, Becker E, Stampfl A. Mechanism of carbon tetrachloride-induced hepatotoxicity. Hepatocellular damage by reactive carbon tetrachloride metabolites. Z Naturforsch 2001;56c:649-59.
21. Ekam VS, Ebong PE. Serum protein and enzyme levels in rats following administration of antioxidant vitamins during caffeinated and non-caffeinated paracetamol-induced hepatotoxicity. Niger J Physiol Sci 2007;22:65-8.
22. Thapa BR, Walia A. Liver function tests and their interpretation. Indian J Pediatr 2007;74:663-71.
23. Hussain L, Ikram J, Rehman K, Tariq M, Ibrahim M, Akash MSH. Hepatoprotective effects of Malva sylvestris L. against paracetamol-induced hepatotoxicity. Turk J Biol 2014;38:396-402.
24. Reddy GVR, Kumar RV, Rama V, Reddy MK, Reddy YN. The preliminary hepatoprotective activity of medicinal plant extracts against carbon tetrachloride-induced hepatotoxicity in albino rats. Int J Recent Sci Res 2015;6:4946-51.
25. Imafidon KE, Okunrobo LO. Study on biochemical indices of liver function tests of albino rats supplemented with three sources of vegetable oils. Nigerian J Basic Appl Sci 2012;20:105-10.
26. Alagammal M, Lincy MP, Mohan VR. Hepatoprotective and antioxidant effect of Polygala rosmarinifolia wight and Arn against CCl4 induced hepatotoxicity in rats. J Pharmacogn Phytochem 2013;2:118-24.
27. Abenavoli L, Capasso R, Milic N, Capasso F. Milk thistle in liver diseases: past, present, future. Phytother Res 2010;24:1423-32.
28. Xiao J, Capanoglu E, Jassbi AR, Miron A. Advance on the flavonoid C-glycosides and health benefits. Crit Rev Food Sci Nutr 2016;56 Suppl 1:29-45.
29. Pan MH, Lai CS, Ho CT. Anti-inflammatory activity of natural dietary flavonoids. Food Funct 2010;1:15-31.
30. Chu X, Wang H, Jiang YM, Zhang YY, Bao YF, Zhang X, et al. Ameliorative effects of tannic acid on carbon tetrachloride-induced liver fibrosis in vivo and in vitro. J Pharmacol Sci 2016;130:15-23.
31. Rufina ASY, Gbadura AI. Protective effect of saponin extract from the root of Garcinia kola (Bitter Kola) against paracetamol-induced hepatotoxicity in albino rats. World Acad Sci Eng Technol Int J Med Health Sci 2015;9:130-4.