• Sholihatil Hidayati Academy of Food and Pharmaceutical Analyses Al-Islam Yogyakarta. Gedongkiwo MJ I/816, 55142, Yogyakarta, Indonesia
  • Rini Sulistyawati Academy of Food and Pharmaceutical Analyses Al-Islam Yogyakarta. Gedongkiwo MJ I/816, 55142, Yogyakarta, Indonesia
  • Laela Hayu Nurani Department of Biology Pharmacy, Faculty of Pharmacy, Ahmad Dahlan University, Jl. Prof. Soepomo Janturan, 55165, Yogyakarta, Indonesia


Objective: Moringa oleifera is a medicinal plant species that has potential as an antidiabetic. The present study was designed to evaluated molecullar mechanism antidiabetic ethyl acetate fraction of Moringa oleifera leaves (EAFML).

Methods: The study was conducted on 30 male wistar rats which were induced with the combination of streptozotocin and nicotinamide by intraperitoneal administration to make a model of type 2 diabetes. After the diabetic induction, all rats were divided into six group and once daily administered orally EAFML in doses standardized quercetine. The treatment was given for ten days, and on the final treatment, all rats were checked their blood glucose level and lipid profile. The skeletal muscles and liver were taken to examine glucose transporter-4 (GLUT4) expression by immunohistochemistry.

Results: The result of this study shows that the blood glucose level in diabetic rats with the treatment of EAFML decreased significantly from 355.8±83.7 mg/dL to 177.5±89.3 mg/dL. The cholesterol decreased significantly from 105.2±47.4 mg/dL to 58.6±6.9 mg/dL and low-density lipoprotein (LDL) in diabetic rats with the treatment of EAFML decreased significantly compared to control group from 175.6±41.9 mg/dL to 95.3±8.0 mg/dL. The expression of GLUT4 in skeletal muscles increased from 0.7±1.0 to 3.9±1.1 and in the liver increased significantly from 1.8±1.3 to 2.9±1.9.

Conclusion: The EAFML can decrease blood glucose level, cholesterol and low-density lipoprotein in type 2 diabetic rat model. Other than that, this fraction can improve insulin sensitivity by the increase of GLUT4 expressions.

Keywords: Ethyl acetate, GLUT4, Moringa oleifera, Type 2 diabetes


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How to Cite
Hidayati, S., R. Sulistyawati, and L. H. Nurani. “REGULATION OF ETHYL ACETATE FRACTION FROM MORINGA OLEIFERA LEAVES TO IMPROVE LIPID METABOLISM AND INSULIN SENSITIVITY IN TYPE 2 DIABETES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 10, Oct. 2018, pp. 78-82, doi:10.22159/ijpps.2018v10i10.27845.
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