A THERMALLY RESPONSIVE SHORT ELASTIN LIKE POLYPEPTIDE-DRUG CONJUGATE: SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION FOR TARGETED DELIVERY OF ANTICANCER DRUGS


Rubha Saxena, M.j. Nanjan

Abstract


Introduction: Among the several new strategies explored today to avoid the side effects in cancer chemotherapy. The concept of polymer-drug conjugates has shown considerable promise. In this context, genetically engineered long elastin like polypeptides (ELPs) have been examined recently as drug carriers. These ELPs, however, have certain limitations.

Objective: It is our hypothesis that short synthetic ELPs can also be used as drug carriers so as to overcome these limitations. The purpose of this investigation was, therefore, to synthesize, characterize and evaluate a thermally responsive short ELP-Doxorubicin conjugate for targeted delivery.

Methods: The ELP-Doxorubicin conjugate of molecular weight 1280 Da was synthesized and characterized by ESI-MS, FTIR and NMR studies. Turbidimetry, differential scanning calorimetry (DSC) and circular dichorism (CD) studies were carried out to evaluate its structural transition behavior. Cellular uptake and intracellular localization studies of the conjugate and the free drug were carried out by flow cytometry and confocal fluorescence microscopy, respectively. In vitro cytotoxicity of the conjugate was evaluated by the MTT assay method and compared with that of the free drug.

Results: The results reveal that the short ELP synthesized exhibits structural transition behavior similar to naturally occurring long ELPs and delivers more drug molecules to intracellular space compared to the free drug. This structural transition behavior can also be exploited for targeting drugs to solid tumors using hyperthermia.

Conclusion: As hypothesized our investigations clearly demonstrate that short thermally responsive ELPs are good carrier for targeting anticancer drugs to the intracellular space.

 


Keywords


Anticancer drug, Drug delivery system, Drug targeting, Elastin like polypeptide, Drug conjugate.

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About this article

Title

A THERMALLY RESPONSIVE SHORT ELASTIN LIKE POLYPEPTIDE-DRUG CONJUGATE: SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION FOR TARGETED DELIVERY OF ANTICANCER DRUGS

Keywords

Anticancer drug, Drug delivery system, Drug targeting, Elastin like polypeptide, Drug conjugate.

Date

01-11-2014

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 6, Issue 10, 2014 Page: 326-333

Online ISSN

0975-1491

Statistics

169 Views | 72 Downloads

Authors & Affiliations

Rubha Saxena
J. S. S. College of Pharmacy
India

M.j. Nanjan
JSS College Of Pharmacy(Off Campus JSS University,Mysore) Ootacamund, The Nilgiris Tamilnadu.


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