SCREENING OF THE ANTIOXIDANT ACTIVITY OF CRUDE EXTRACTS IN 86 ALGAE SPECIES FROM EL JADIDA COAST (MOROCCO)

  • Fatima Chibi Biotechnology and Valorization of Plant Resources: Algae and Plants, Department of Biology, Faculty of Sciences, Chouaib Doukkali University, El Jadida, Morocco
  • Halima Rchid Biotechnology and Valorization of Plant Resources: Algae and Plants, Department of Biology, Faculty of Sciences, Chouaib Doukkali University, El Jadida, Morocco
  • Wafa Arsalane Biotechnology and Valorization of Plant Resources: Algae and Plants, Department of Biology, Faculty of Sciences, Chouaib Doukkali University, El Jadida, Morocco
  • Rachid Nmila Biotechnology and Valorization of Plant Resources: Algae and Plants, Department of Biology, Faculty of Sciences, Chouaib Doukkali University, El Jadida, Morocco

Abstract

Objective: This work aimed to screen the antioxidant activity of marine macroalgae from the Moroccan Atlantic coast (region of El Jadida).


Methods: Evaluation of the antioxidant activity of different collected species, lyophilized and extracted with a solvent mixture chloroform/methanol (2/1; v/v) was conducted according to two techniques, first by thin layer chromatography (tlc) then by spectrophotometry, using a free radical 2,2-diphenyl-1-picrylhydrazyl (dpph). The sampling on a distance of 110 km allowed to harvest 86 algal species (16 brown algae, 47 red algae, 14 green algae and 9 algae being identified).


Results: The analysis by thin layer chromatography reveals an antioxidant activity in nearly half of harvested algal species (52.32 %). This activity varies depending on the concentration of the extract and in function of incubation time in the presence of dpph. The monitoring of the kinetics of degradation of dpph by spectrophotometer in the presence of extracts which were active by tlc allowed to confirm the results and select the most active algal species based on the percentage of remaining dpph in the medium after 120 min of reaction: Fucus spiralis (17.02 %), Cyctoseira ericoides (12.16 %) (Phaeophyceae), and Gracilaria multipartita (36%), Halopitys incurvus (5%) (Rhodophyceae).


Conclusion: The results show that the methodology adopted in this work is reliable and can be used for rapid screening of antioxidant property in plants and the species: Fucus spiralis, Cyctoseira ericoides, Gracilaria multipartita, and Halopitys incurvus can be a promising source of natural compounds endowed with high antioxidant potential.

Keywords: Marine algae, Morocco, Extracts, Antioxidant activity, Dpph

Downloads

Download data is not yet available.

Author Biography

Rachid Nmila, Biotechnology and Valorization of Plant Resources: Algae and Plants, Department of Biology, Faculty of Sciences, Chouaib Doukkali University, El Jadida, Morocco

Departement of life sciences

References

1. Liu L, Heinrich M, Myers S, Dworjanyn SA. Towards a better understanding of medicinal uses of the brown seaweed Sargassum in Traditional Chinese Medicine: a phytochemical and pharmacological review. J Ethnopharm 2012;14:591-619.
2. El Gamal AA. Biological importance of marine algae. Saudi Pharm J 2010;18:1-25.
3. Stengel DB, Connan S, Popper ZA. Algal chemodiversity and bioactivity: Sources of natural variability and implications for commercial application. Biotechnol Ad 2011;29:483–501.
4. Kuda T, Tsunekawa M, Goto H, Araki Y. Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan. J Food Compos Anal 2005;18:625–33.
5. Shalaby EA. Algae as promising organisms for environment and health. Plant Signaling Behav 2011;6:1338-50.
6. Zandi K, Fouladvand M, Pakdel P, Sartavi K. Evaluation of in vitro antiviral activity of a brown alga (Cystoseira myrica) from the persian gulf against herpes simplex virus type 1. Afr J Biotechnol 2007;6:2511-4.
7. Lekameera R, Vijayabaskar P, Somasundaram ST. Evaluating antioxidant property of brown alga Colpomenia sinuosa (DERB. ET SOL). Afr J Food Sci 2008;2:126-30.
8. Taskin E, Caki Z, Ozturk M, Taskin E. Assessment of in vitro antitumoral and antimicrobial activities of marine algae harvested from the eastern mediterranean sea. Afr J Biotechnol 2010;9:4272-7.
9. Ashwini S, Suresh babut V, Sarith A, Manjula S. Seaweed extracts exhibit anticancer activity against HeLa cell lines. Int J Curr Pharm Res 2017;9:114-7.
10. Jayasree P, Thiruchelvi R, Balashanmugam P. Evaluation of antibacterial, antioxidant, and anticancer potentials from marine red algae Gracilaria corticata. Asian J Pharm Clin Res 2018;1:347-50.
11. Dore CMPG, Faustino Alvesa MGC, Pofírio Will LSE, Costa TG, Sabry DA, de Souza Rego LAR, et al. A sulfated polysaccharide, fucans, isolated from brown algae Sargassum vulgare with anticoagulant, antithrombotic, antioxidant and anti-inflammatory effects. Carbohydr Polym 2013;91:467-75.
12. Matsukawa R, Dubinsky Z, Kishimoto E, Masaki K, Masuda Y, Takeuchi T, et al. A comparison of screening methods for antioxidant activity in seaweeds. J Appl Phycol 1997;9:29-35.
13. Connan S, Deslandes E, Ar Gall E. Influence of day–night and tidal cycles on phenol content and antioxidant capacity in three temperate intertidal brown seaweeds. J Experimental Mar Biol Ecol 2007;349:359-69.
14. Nahas R, Abatis D, Anagnostopoulou MA, Kefalas P, Vagias C, Roussis V. Radical-scavenging activity of aegean sea marine algae. Food Chem 2007;102:577-81.
15. Abd El Mageid MM, Salama NA, Saleh MAM, Abo Taleb HM. Antioxidant and antimicrobial characteristics of red and brown algae extracts. 4th Conference on Recent Technologies in Agriculture; 2009. p. 818-28.
16. Demirel Z, Yilmaz Koz FF, Karabay Yavasoglu UN, Ozdemir G, Sukatar A. Antimicrobial and antioxidant activity of brown algae from the Aegean Sea. J Serb Chem Soc 2009;74:619-28.
17. Zubia M, Fabre MS, Kerjean V, Le Lann K, Stiger Pouvreau V, Fauchon M, et al. Antioxidant and antitumoural activities of some phaeophyta from brittany coasts. Food Chem 2009;116:693-701.
18. Ayyad SEN, Makki MS, Al-kayal NS, Basaif SA, El-Foty KO, Asiri AM, et al. Cytotoxic and protective DNA damage of three new diterpenoids from the brown alga Dictoyota dichotoma. Eur J Med Chem 2011;46:175-82.
19. Kelman D, Kromkowski Posner E, McDermid KJ, Tabandera NK, Wright PR, Wright AD. Antioxidant activity of hawaiian marine algae. Mar Drugs 2012;10:403-16.
20. Vijayabaskar P, Shiyamala V. Antioxidant properties of seaweed polyphenol from Turbinaria ornate (Turner) J. Agardh, 1848. Asian Pacific J Tropical Biomed 2012;S:90-8.
21. Andrade PB, Barbosa M, Matos RP, Lopes G, Vinholes J, Mouga T, et al. Valuable compounds in macroalgae extracts. Food Chem 2013;138:1819-28.
22. Farvin KHS, Jacobsen C. Phenolic compounds and antioxidant activities of selected species of seaweeds from danish coast. Food Chem 2013;138:1670-81.
23. Yuan YV, Bone DE, Carrington ME. Antioxidant activity of dulse, Palmaria palmata, extract evaluated in vitro. Food Chem 2005;91:485-94.
24. Plaza M, Cifuentes A, Ibanez E. In the search of new functional food ingredients from algae. Trends Food Sci Tech 2008;19:31-9.
25. Ananthi S, Raghavendran HRB, Sunil AG, Gayathri V, Ramakrishnan G, Vasanthi HR. In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata (brown alga). Food Chem Toxicol 2010;48:187-92.
26. Vijayabaskar P, Vaseela N, Thirumaran G. Potential antibacterial and antioxidant properties of a sulfated polysaccharide from the brown marine algae Sargassum swartzii. Chin J Nat Med 2012;10:0421?8.
27. Etahiri S, Bultel Ponce V, Caux C, Guyot M. New bromoditerpenes from the red alga Sphaerococcus coronopifolius. J Nat Prod 2001;64:1024-7.
28. Chiheb I, Riadi H, Martinez Lopez J, Dominguez Seglar JF, Gomez Vidal JA, Bouziane H, et al. Screening of antibacterial activity in marine green and brown macroalgae from the coast of morocco. Afr J Biotechnol 2009;8:1258-62.
29. Younes F, Etahiri S, Assobhei O. Activité antimicrobienne des algues marines de la lagune d’Oualidia (Maroc): criblage et optimisation de la periode de la récolte. J Appl Biosci 2009;24:1543-52.
30. Bouhlal R, Riadi H, Bourgougnon N. Antiviral activity of the extracts of rhodophyceae from. Morocco Afr J Biotech 2010;9:7968-75.
31. Farid Y, Chennaoui M, Assobhei O, Etahiri S. Evaluation de l’effet du lieu de recolte des algues marines des cotes atlantiques marocaines sur L’activite antibacterienne et anti–inflammatoire. Microbiol Ind San Environn 2012;6:54-66.
32. Oumaskour K, Boujaber N, Etahiri S, Assobhei O. Screening of antibacterial and antifungal activities in green and brown algae from the coast of Sidi Bouzid (El Jadida, Morocco). Afr J Biotech 2012;11:16831-7.
33. Valls R, Piovetti L, Praud A. The use of diterpenoids as chemotaxonomic markers in the genus Cystoseira. Hydrobiologia 1993;26:549-56.
34. Bouhlal R, Riadi H, Bourgougnon N. Antioxidant activity of rhodophyceae extracts from atlantic and mediterranean coasts of morocco. Afr J Plant Sci 2013;7:110-7.
35. Takao T, Kitatami F, Watanabe N, Yagi A, Sakata K. A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shell fish. Biosci Biotechnol Biochem 1994;58:1780-3.
36. Brand Williams W, Cuvelier E, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995;28:25-30.
37. Benhissoune S. Contribution à letude de la flore des macroalgues marines du Maroc (mediterranee et atlantique). PhD Thesis, Mohammed V University; 2002.
38. Givernand T, Sqali N, Barbaroux O, Orbi A, Semmaoui Y, Rezzoum NE, et al. Mapping and biomass estimation for a harvested population of Gelidium sesquipedale (Rhodophyta, Gelidiales) along the Atlantic coast of Morocco. Phycologia 2005;44:66-71.
39. Hanif N, Chair M, Idrissi MC, Naoki T. Contribution to the algal biodiversity study in the moroccan atlantic coast. Int J Innov Res Sci Eng Tech 2014;3:12507-24.
40. Hanif N, Chair M, Idrissi MC, Naoki T. L'exploitation des algues rouges Gelidium dans la région d’El Jadida: aspects socio-économiques et perspectives. Afr Sci 2014;10:103-26.
41. Heo SJ, Cha SH, Lee KW, Jeon YJ. Antioxidant activities of red algae from jeju island. Algae 2006;21:149-56.
42. Ragubeer N, Beukes DR, Limson JL. Critical assessment of voltammetry for rapid screening of antioxidants in marine algae. Food Chem 2010;121:227–32.
43. Tierney MS, Smyth TJ, Rai DK, Soler Vil A, Croft AK, Brunton N. Enrichment of polyphenol contents and antioxidant activities of Irish brown macroalgae using food-friendly techniques based on polarity and molecular size. Food Chem 2013;139:753-61.
44. Balboa EM, Conde E, Moure A, Falque E, Dominguez H. In vitro antioxidant properties of crude extracts and compounds from brown algae. Food Chem 2013;138:1764–85.
45. Martins CDL, Ramlov F, Carneiro NPN, Gestinari LM, Santos BFD, Bento LM, et al. Antioxidant properties and total phenolic contents of some tropical seaweeds of the Brazilian coast. J Appl Phycol 2012;25:1179–87.
46. Meenakshi S, Umayaparvathi S, Arumugam M, Balasubramanian T. In vitro antioxidant properties and FTIR analysis of two seaweeds of gulf of mannar. Asian Pacific J Tropical Biomed 2011;1:S66–S70.
47. Ruberto G, Baratta MT, Biondi DM, Amico V. Antioxidant activity of extracts of the marine algal genus Cystoseira in a micellar model system. J Appl Phycol 2001;13:403–7.
48. Cerantola S, Florian B, Erwan AG, Deslandes E. Co-occurrence and antioxidant activities of fucol and fucophlorethol classes of polymeric phenols in Fucus spiralis. Bot Mar 2006;49:347–51.
49. Pinteus S, Azevedo S, Alves C, Mouga T, Cruz A, Afonso C, et al. High antioxidant potential of Fucus spiralis extracts collected from peniche coast (Portugal). New Biotechnol 2009;25:S296.
50. Ferreres F, Lopes G, Gil-Izquierdo A, Andrade PB, Sousa C, Mouga T, et al. Phlorotannin extracts from fucales characterized by HPLC-DAD-ESI-MS: approaches to hyaluronidase inhibitory capacity and antioxidant properties. Mar Drugs 2012;10:2766-81.
51. Grozdanic N, Stanojkovic TP, Kljajic Z, Etahiri S, Assobhei O, Konic Ristic A, et al. In vitro evaluation of antioxidant and antitumoral activities of marine algae Gelidium Sesquipedale and Fucus Spiralis. Eur J Cancer 2012;48:S26.
52. Plaza M, Amigo Benavent M, del Castillo MD, Ibanez E, Herrero M. Facts about the formation of new antioxidants in natural samples after subcritical water extraction. Food Res Int 2010;43:2341–8.
53. Satyalakshmi S. Determination of biological activities of three marine algae collected from visakhapatnam coast. Asian J Pharm Clin Res 2017;10:274-9.
Statistics
48 Views | 170 Downloads
How to Cite
Chibi, F., H. Rchid, W. Arsalane, and R. Nmila. “SCREENING OF THE ANTIOXIDANT ACTIVITY OF CRUDE EXTRACTS IN 86 ALGAE SPECIES FROM EL JADIDA COAST (MOROCCO)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 3, Jan. 2019, pp. 54-61, doi:10.22159/ijpps.2019v11i3.28666.
Section
Original Article(s)