EVALUATION OF ANTIOXIDANT AND CHEMOPREVENTIVE POTENTIAL OF METHANOLIC EXTRACTS OF LEAF OF AEGLEMARMELOS ATTRIBUTES TOWARDS DUCTAL CARCINOMA STUDIED IN MCF7 CELLS
Objective: The objective of the present research work had been made to evaluate the antioxidant potential along with anti-cancer activity of methanolic extracts from the leaf of A. marmelos.
Methods: Standard methods for antioxidant potential in terms of DPPH and nitric oxide scavenging assay, and anticancer activity in vitro method (Cytotoxicity/MTT assay and % of cell viability) by using MCF7 cell line.
Results: Results of antioxidant efficacy revealed that the IC50 value for DPPH and nitric oxide scavenging assay was considered to be 62.032%, and 20.69% respectively. The methanolic extract of A. marmelos was found to possess enhanced anticancer potential against MCF7 cells. Cytotoxicity activity of MCF7 cells, when treated with methanolic extract of A. marmelos, was found to be 43.42% at 25µg, 52.31% at 50µg, 56.31% at 75µg, 58.38% at 100µg, 62.25% at 125µg. The IC50 value was found as 49.36µg. Toxicity was significantly increased with increased concentration and viability significantly decreased with the increased concentration of methanolic extract of leaf from A. marmelos for MCF7 cell when compared to cyclophosphamide.
Conclusion: From the studies, it was postulated that methanolic extract of leaf from A. marmeloshas significant chemopreventive activity. These specific identities will be useful for the identification and authentication of raw drug.
2. Peedell C. Concise clinical oncology. Elsevier: Philadelphia USA; 2005. p. 3-5.
3. Asolkar LV, Kakkar KK, Charkre OJ. Second supplements to glossary of Indian medicinal plants with active principles, Part-I (A-K) (1965-1981), Published by Publications and Information Directorate (CSIR), New Delhi, India; 1992. p. 217.
4. Vaiyapuri Subbarayan Periasamy, Jegan Athinarayanan, Ali A Alshatwi, Ultrasonics Sonochemistry. Anticancer activity of an ultrasonic nanoemulsion formulation of Nigella sativa L Essential oil on human breast cancer cells. Ultrason Sonochem 2016;31:449-55.
5. WHO Traditional medicine: Growing needs and potential, WHO Policy perspectives on medicines. World Health Organisation, Geneva; 2002. p. 1.
6. Mandal S, Yadav S, Yadav S, Nema RK. Antioxidants: a review. J Chem Pharm Res 2009;1:102-4.
7. Ajitha M, Rajnarayana K. Role of oxygen free radicals in human disease. Indian Drugs 2001;38:545-54.
8. Halliwell B, Aeschbach R, Loliger J, Aruom OI. The characterization of antioxidants. Food Chem Toxicol 1995;33:601-17.
9. Gamble JS. The Flora of the presidency of madras. Botanical Survey India 1967;2:524.
10. Brain KR, Turner TD. The practical evaluation of phytopharmaceuticals Bristol: Wright Scientehnica; 1975. p. 78-80.
11. Gupta AP, Verma RK, Gupta MM, Sunilkumar. Estimation of plumbagin using high performance, thinlayer chromatography. J Med Arom PI Sci 1999;21:661-3.
12. Nenadis N, Tsimidou M. Observations on the estimation of scavenging activity of phenolic compounds using rapid 1, 1-diphenyl-2-picrylhyrazyl [DPPH!]. J Am Oil Chem Soc 2002;79:1191-5.
13. Sreejayan N, Rao MNA. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 1997;49:105-107.
14. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity test. J Immunol Methods 1983;65:55-63.
15. Ebrahimzadeh MA, Pourmorad F, Hafezi S. Antioxidant activities of Iranian corn silk Turk. J Biol 2008b;32:43-9.
16. Ebrahimzadeh MA, Bahramian F. Antioxidant activity of Crataeguspentagina subspecies Elbursis fruits extracts are used in traditional medicine in Iran Pak. J Biol Sci I 2009a;12:413-9.
17. Ebrahimzadeh MA, Ehsanifar S, Eslami B. Sambucusebuluselburensis fruit: a good source for antioxidants. Pharmacogn Mag 2009b;4:213-8.
18. Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Eslami B. Antioxidant activity of aqueous extract of Pyrusboissieriana fruit. Pharmacologyonline 2009c;1:1318-23.
19. Bashir Ado Ahmad, Khamsah Suryati Mohd, Muhammad Abdurrazak, US Mahadeva Rao, Thant Zin. Phytochemical screening, the antioxidant activity of pure syringinin comparison to various solvents extracts of Musa paradisiaca (Banana) (Fruit and flower) and total phenolic contents. Int J Pharm Pharm Sci 2015;7:242-7.
20. Tylor BS, Kion YM, Wang QI, Sharpio RA, Billiar TR, Geller DA. Nitric oxide Down-regulates hepatocyte-inducible nitric oxide synthase gene expression. Arcg Surg 1997;132:1177-83.
21. Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Hamidinia A, Bekhradnia AR. Determination of antioxidant activity, phenol and flavonoids content of Parrotiapersica Mey. Pharmacol Online 2008a;2:560-7.
22. Benni JM, Jayanthi MK, Suresha RN. Evaluation of anti-inflammatory activity of Aeglemarmelos (Bilwa) root. Indian J Pharmacol 2011;43:393-7.
23. Horiuchi N, Nakagava K, Sasaki Y, Minato K, Fujiwara Y, Nezu K, et al. In vitro antitumor activity of mitomycin C derivative (RM-49) and a new anticancer antibiotic (FK973) against lung cancer cell lines determined by tetrazolium dye (MTT) assay. Cancer Chemother Pharmacol 1988;22:246-50.
24. Lambertini E, Piva R, Khan MT, Lampronti I, Bianchi N, et al. Effects of extracts from Bangladeshi medicinal plants on in vitro proliferation of human breast cancer cell lines and expression of estrogen receptor alpha gene. Int J Oncol 2004;24:419-23.
25. Jagetia GC, Venkatesh P, Baliga MS. Aeglemarmelos correa inhibits the proliferation of transplanted Ehrlich ascites carcinoma in mice. Biol Pharm Bull 2005;28:58-64.
26. Kaniz Fatima Urmi, Ashraf Uddin Chowdhury, Farhana Diba, Khandaker Ashfaqur Rahman. Evaluation of antioxidant and cytotoxic potential of different extracts from the leaves of Aeglemarmelos. Res J Pharm Tech 2013;6:384-7.