EVALUATION OF PHYTASE PRODUCTION BY HYPOCREA LIXII SURT01 IN SUBMERGED AND SOLID-STATE FERMENTATIONS

  • Thyagarajan R Department of Biotechnology, Faculty of Bio-engineering, Sathyabama University, Chennai -600045
  • Karthick Raja Namasivayam S Department of Biotechnology, Faculty of Bio-engineering, Sathyabama University, Chennai -600045
  • Narendrakumar G Department of Biotechnology, Faculty of Bio-engineering, Sathyabama University, Chennai -600045

Abstract

Objective: Phytases have important applications in human and animal nutrition because they hydrolyze the phytate present in legumes, cereal grains and oil seeds to release inorganic phosphate. Supplementation of phosphate to the poultry causes a serious problem of eutrophication. This can be reduced by incorporating phytase in poultry feed. Present study explains extracellular phytase production by SmF and SSF from a fungal strain Hypocrea lixii SURT01.

Methods: Extracellular phytase production by Hypocrea lixii SURT01 was evaluated in media containing various refined carbon sources (Fructose, Sucrose, Maltose and lactose in concentration ranging from 1.5% to 7.5%) along with standard medium under submerged fermentation (SmF). At the same time, phytase production was studied under Solid State Fermentation (SSF) with four different substrate such as barley, green gram, bengal gram and black gram.

Results: In SmF out of different carbon sources in various concentrations, 6% sucrose showed maximum enzyme production (245U/ml). In SSF, barley showed highest phytase yield (1638 Units/ml) on 5th day of incubation.

Conclusion: Evaluation of Solid state fermentation showed enhanced phytase production when compared to Submerged Fermentation.

 

Keywords: Phytase, Submerged fermentation, Solid state fermentation, Enzyme activity.

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How to Cite
R, T., K. R. N. S, and N. G. “EVALUATION OF PHYTASE PRODUCTION BY HYPOCREA LIXII SURT01 IN SUBMERGED AND SOLID-STATE FERMENTATIONS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 10, 1, pp. 352-5, https://innovareacademics.in/journals/index.php/ijpps/article/view/2903.
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