• S. MANIKANDAN Department of Marine and Coastal Studies, School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai 625021, Tamilnadu, India,
  • S. GANESAPANDIAN Government Higher Secondary School, Kadukkai valasai, Ramanathapuram District, Tamilnadu, India
  • MANOJ SINGH Department of Marine and Coastal Studies, School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai 625021, Tamilnadu, India. Department of Biotechnology, MMEC, Maharishi Markandeshwar (Deemed to be University), Mullana 133207, Haryana, India
  • A. K. KUMARAGURU Department of Marine and Coastal Studies, School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai 625021, Tamilnadu, India


Objective: The present study was conducted to screen the cytotoxicity and initiation of apoptosis in MCF-7 human breast tumour cells using the Indian sponge Acanthostylotela cornuta of the Gulf of Mannar.

Methods: The crude methanol extract of A. cornuta was fractionated using a reversed phase silica gel column chromatography. The purity of the active fraction of bromopyrrole alkaloids was confirmed with Gas Chromatography-Mass Spectrometry (GCMS). The bromopyrrole alkaloids induce apoptotic changes in MCF-7 cells were studied with electrophoresis, caspase assay, and different staining analysis. The MCF-7 cells were analyzed by Flow cytometry to determine their DNA content.

Results: Bromopyrrole alkaloids of A. cornuta showed cytotoxicity against MCF-7 human breast tumour cells with the IC50 value of 8.0μg/ml. The bromopyrrole compounds induced cells exhibited scatted red fluorescence, showing the presence of several residual bodies and condensation of chromatin. Caspase-3, pro-caspase-9, caspase-9 and Poly-ADP-Ribose-Polymerase (PARP) activity were occurred in bromopyrrole alkaloids treated tumour cell. The cell cycle arrest is conceivable that the compound prevented the progression of cell cycle through the G phase resulting from inhibition of survival, leading them to undergo apoptosis.

Conclusion: Bromopyrrole alkaloids of A. cornuta possess antitumor activity which was arrested the G phase in cell cycle that clearly indicated its nature as that of antitumour drugs.

Keywords: Marine sponge, Acanthostylotela cornuta, Antitumour, Breast tumour, Apoptosis


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How to Cite
MANIKANDAN, S., S. GANESAPANDIAN, M. SINGH, and A. K. KUMARAGURU. “ANTI-TUMOUR ACTIVITY OF BROMOPYRROLE ALKALOIDS AGAINST HUMAN BREAST TUMOUR (MCF-7) THROUGH APOPTOSIS INDUCTION”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 6, May 2019, pp. 94-100, doi:10.22159/ijpps.2019v11i6.29092.
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